Abstract
The 16S rRNA gene approach was applied to investigate the diversity of Planctomycetes in latitudinal surface seawater of the Western Pacific Ocean. The results revealed that the Pirellula-Rhodopirellula-Blastopirellula clade dominated the Planctomycetes community at all surface seawater sites while the minority genera Gemmata and Planctomyces were only found at sites H5 and H2 respectively. Although the clone frequency of the PRB clade seemed stable (between 83.3% and 94.1%) for all surface seawater sites, the retrieved Pirellula-Rhodopirellula-Blastopirellula clade presented unexpected diversity. Interestingly, low latitude seawater appeared to have higher diversity than mid-latitudes. ∫-LIBSHUFF software analysis revealed significantly different diversity patterns between in latitudinal surface seawater and in the sediment of South China Sea station M2896. Our data suggested that different hydrological and geographic features contributed to the shift of Planctomycetes diversity in marine environments. This is, to our knowledge, the first systematic assessment of Planctomycetes in latitudinal surface seawater of the open sea and the first comparison of diversity pattern between surface seawater and sediments and has broadened our understanding of Planctomycetes diversity in marine environments.
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References
Bauer, M., T. Lombardot, H. Teeling, N.L. Ward, R. Amann, and F. Glockner. 2004. Archaea-like genes for C-1-transfer enzymes in Planctomycetes: Phylogenetic implications of their unexpected presence in this phylum. J. Mol. Evol. 59, 571–586.
Brummer, I.H.M., A.D.M. Felske, and I. Wagner-Dobler. 2004. Diversity and seasonal changes of uncultured Planctomycetales in river biofilms. Appl. Environ. Microbiol. 70, 5094–5101.
Buckley, D.H., V. Huangyutitham, T.A. Nelson, A. Rumberger, and J.E. Thies. 2006. Diversity of Planctomycetes in soil in relation to soil history and environmental heterogeneity. Appl. Environ. Microbiol. 72, 4522–4531.
Chouari, R., D. Le Paslier, P. Daegelen, P. Ginestet, J. Weissenbach, and A. Sghir. 2003. Molecular evidence for novel Planctomycete diversity in a municipal wastewater treatment plant. Appl. Environ. Microbiol. 69, 7354–7363.
Elshahed, M.S., N.H. Youssef, Q.W. Luo, F.Z. Najar, B.A. Roe, T.M. Sisk, S.I. Buhring, K.U. Hinrichs, and L.R. Krumholz. 2007. Phylogenetic and meta-bolic diversity of Planctomycetes from anaerobic, sulfide-and sulfur-rich Zodletone Spring, Oklahoma. Appl. Environ. Microbiol. 73, 4707–4716.
Fine, R.A., R. Lukas, F.M. Bingham, M.J. Warner, and R.H. Gammon. 1994. The western equatorial Pacific: A water mass crossroads. J. Geophys. Res. 99, 25063–25080.
Freitag, T.E. and J.I. Prosser. 2003. Community structure of ammonia-oxidizing bacteria within anoxic marine sediments. Appl. Environ. Microbiol. 69, 1359–1371.
Fuerst, J.A. 1995. The planctomycetes: emerging models for microbial ecology, evolution and cell biology. Microbiology 141, 1493–1506.
Huang, R.X. and Q. Wang. 2001. Interior communication from the subtropical to the tropical oceans. J. Phys. Oceanogr. 31, 3538–3550.
Ivanova, A.O. and S.N. Dedysh. 2006. High abundance of Planctomycetes in anoxic layers of a Sphagnum peat bog. Microbiology 75, 716–719.
Jiao, N.Z., Y.H. Yang, H. Koshigawa and M. Watanabe. 2002. Influence of hydrographic conditions on picoplankton distribution in the East China Sea, a marginal sea of the Northwest Pacific. Aquat. Microb. Ecol. 30, 37–48.
Kartal, B., J. Rattray, L.A. Van Niftrik, J. Van De Vossenberg, M.C. Schmid, R.I. Webb, S. Schouten, J.A. Fuerst, J.S.S. Damste, M.S.M. Jetten, and M. Strous. 2007. Candidatus “Anammoxoglobus propionicus” a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria. Syst. Appl. Microbiol. 30, 39–49.
Kirkpatrick, J., B. Oakley, C. Fuchsman, S. Srinivasan, J.T. Staley, and J.W. Murray. 2006. Diversity and distribution of Planctomycetes and related bacteria in the suboxic zone of the Black Sea. Appl. Environ. Microbiol. 72, 3079–3083.
Konig, E., H. Schlesner, and P. Hirsch. 1984. Cell-wall studies on budding bacteria of the Planctomycetes pasteuria group and on a Prosthecomicrobium sp., Arch. Microbiol. 138, 200–205.
Lim, A. and L.X. Zhang, 1999. WebPHYLIP: a web interface to PHYLIP. Bioinformatics 15, 1068–1069.
Lindsay, M.R., R.I. Webb, and J.A. Fuerst. 1997. Pirellulosomes: A new type of membrane-bounded cell compartment in Planctomycete bacteria of the genus Pirellula. Microbiology 143, 739–748.
Miskin, I.P., P. Farrimond, and I.M. Head. 1999. Identification of novel bacterial lineages as active members of microbial populations in a freshwater sediment using a rapid RNA extraction procedure and RT-PCR. Microbiology 145, 1977–1987.
Morris, R.M., K. Longnecker, and S.J. Giovannoni. 2006. Pirellula and OM43 are among the dominant lineages identified in an Oregon coast diatom bloom. Appl. Environ. Microbiol. 8, 1361–1370.
Ronquist, F. and J.P. Huelsenbeck. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.
Schlesner, H., C. Rensmann, B.J. Tindall, D. Gade, R. Rabus, S. Pfeiffer, and P. Hirsch. 2004. Taxonomic heterogeneity within the Planctomycetales as derived by DNA-DNA hybridization, description of Rhodopirellula baltica gen. nov., sp. nov., transfer of Pirellula marina to the genus Blastopirellula gen. nov. as Blastopirellula marina comb. nov. and emended description of the genus Pirellula. Int. J. Syst. Evol. Microbiol. 54, 1567–1580.
Schloss P.D., B.R. Larget, and J. Handelsman. 2004. Integration of microbial ecology and statistics: a test to compare gene libraries. Appl. Environ. Microbiol. 70, 5485–5492.
Schmid, M., K. Walsh, R. Webb, W.I.C. Rijpstra, K. Van De Pas-Schoonen, M.J. Verbruggen, T. Hill, B. Moffett, J. Fuerst, S. Schouten, J.S.S. Damste, J. Harris, P. Shaw, M. Jetten, and M. Strous. 2003. Candidatus “Scalindua brodae”, sp. nov., Candidatus “Scalindua wagneri”, sp. nov., two new species of anaerobic ammonium oxidizing bacteria. Syst. Appl. Microbiol. 26, 529–538.
Stachebrandt, E., W. Ludwig, W. Schubert, F. Klink, H. Schlesner, T. Roggentin, and P. Hirsch. 1984. Molecular genetic evidence for early evolutionary origin of budding pepetidoglycan-less eubacteria. Nature 307, 735–737.
Staley, J.T. 1973. Budding bacteria of the Pasteuria-Blastobacter group. Can. J. Microbiol. 19, 609–614.
Tadonleke, R.D. 2007. Strong coupling between natural Planctomycetes and changes in the quality of dissolved organic matter in freshwater samples. FEMS Microbiol. Ecol. 59, 543–555.
Yang, G.P., X.L. Liu, and J.W. Zhang. 1998. Distribution of dibenzothiophene in the sediments of the South China Sea. Environ. Pollut. 101, 405–414.
Zhou, H., J.P. Jian, P.F. Guo, M.C. Shi, Z.H. Liu, and Y.F. Li. 2006. A study on an anticyclonic eddy east of Mindanao observed by an Argo profiling float. J. Trop. Oceano. 25, 8–14.
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Shu, Q., Jiao, N. Different Planctomycetes diversity patterns in latitudinal surface seawater of the open sea and in sediment. J Microbiol. 46, 154–159 (2008). https://doi.org/10.1007/s12275-008-0002-9
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DOI: https://doi.org/10.1007/s12275-008-0002-9