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Optimization of pancreatic lipase inhibitory and antioxidant activities of Ilex paraguariensis by using response surface methodology

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Abstract

Response surface methodology (RSM) using a Box-Behnken design was used to optimize the extraction conditions for obtaining pancreatic lipase inhibitory and antioxidant principles from Ilex paraguariensis leaves. Three influencing factors: extraction time (min), the liquid–solid ratio, and ethanol concentration (%, v/v) were investigated in the ultrasonic extraction process. Optimization of the extraction conditions to obtain a product with minimum PL activity, maximum antioxidant activity, and maximum yield was performed using RSM by focusing on the three target influencing factors. The optimum conditions were established as the ethanol concentration (54.8 %), liquid–solid ratio (35.4), and extraction time (70.0 min). Under these conditions, the 2,2-diphenyl-1-picrylhydrazyl scavenging activity, PL activity, extraction yield were 59.3 ± 3.5, 35.3 ± 3.0, and 34.4 ± 0.4 %, respectively, similar to the theoretical predicted values of 59.7, 35.2, and 34.3 %, respectively.

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Acknowledgments

This work was supported by the Grants (NRF-2014R1A1A1003605 and NRF-2014R1A4A1007304) from the National Research Foundation of Korea funded by the Korean Government.

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Authors and Affiliations

  1. College of Pharmacy, Korea University, Sejong, 339-700, Republic of Korea

    Kyung-Eon Oh, Hyeji Shin, Young Ho Jeon & Ki Yong Lee

  2. College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea

    Yang Hee Jo & Mi Kyeong Lee

  3. Department of Chemistry, Jackson State University, Jackson, MS, 39217, USA

    Ken S. Lee

  4. College of Pharmacy, Keimyung University, Daegu, 704-701, Republic of Korea

    Byoungduck Park

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  1. Kyung-Eon Oh
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  2. Hyeji Shin
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  3. Young Ho Jeon
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  4. Yang Hee Jo
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  5. Mi Kyeong Lee
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  6. Ken S. Lee
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  8. Ki Yong Lee
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Correspondence to Byoungduck Park or Ki Yong Lee.

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Oh, KE., Shin, H., Jeon, Y.H. et al. Optimization of pancreatic lipase inhibitory and antioxidant activities of Ilex paraguariensis by using response surface methodology. Arch. Pharm. Res. 39, 946–952 (2016). https://doi.org/10.1007/s12272-016-0768-y

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  • DOI: https://doi.org/10.1007/s12272-016-0768-y

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