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Antiplatelet, anticoagulant, and profibrinolytic activities of baicalin

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Abstract

Baicalin is a flavonoid compound purified from the medicinal plant Scutellaria baicalensis Georgi and has been reported to possess anti-inflammatory and anti-viral activities. However, antiplatelet, anticoagulant, and profibrinolytic properties of baicalin have not been studied. In this study, the anticoagulant and antiplatelet activities of baicalin were measured by monitoring activated partial thromboplastin-time (aPTT), prothrombin time (PT), the activities of cell-based thrombin and activated factor X (FXa), platelet aggregation and thrombus formation. The effects of baicalin on the expressions of plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) were also tested in tumor necrosis factor-α (TNF-α) activated human umbilical vein endothelial cells (HUVECs). Our data showed that baicalin inhibited thrombin-catalyzed fibrin polymerization and platelet functions, prolonged aPTT and PT significantly and inhibited the activities and production of thrombin and FXa. Baicalin also prolonged in vivo bleeding time and inhibited TNF-α induced PAI-1 production. Furthermore, PAI-1/t-PA ratio was significantly decreased by baicalin. Collectively, these results indicate that baicalin possesses antithrombotic activities and suggest that the current study could provide bases for the development of new anticoagulant agents.

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Acknowledgments

This study was supported by the National Research Foundation of Korea (NRF) funded by the Korea government [MSIP] (Grant no. 2013-067053).

Conflict of interest

The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea

    Wonhwa Lee & Jong-Sup Bae

  2. BK21 Plus KNU Biomedical Convergence Program, Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Wonhwa Lee

  3. Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea

    Sae-Kwang Ku

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  1. Wonhwa Lee
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Correspondence to Jong-Sup Bae.

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W. Lee and S.-K. Ku contributed equally to this work.

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Lee, W., Ku, SK. & Bae, JS. Antiplatelet, anticoagulant, and profibrinolytic activities of baicalin. Arch. Pharm. Res. 38, 893–903 (2015). https://doi.org/10.1007/s12272-014-0410-9

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