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Antiplatelet, anticoagulant, and profibrinolytic activities of cudratricusxanthone A

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Abstract

Cudratricusxanthone A (CTXA), a natural bioactive compound extracted from the roots of Cudrania tricuspidata Bureau, is known to possess hepatoprotective, antiproliferative and anti-inflammatory activities. However, antiplatelet, anticoagulant, and profibrinolytic properties have not been studied. The anticoagulant activities of CTXA were measured by monitoring activated partial thromboplastin-time (aPTT), prothrombin time (PT), and the activities of cell-based thrombin and activated factor X (FXa). The effects of CTXA on the expressions of plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) were also tested in tumor necrosis factor-α (TNF-α) activated human umbilical vein endothelial cells. Our data showed that CTXA inhibited thrombin-catalyzed fibrin polymerization and platelet aggregation, prolonged aPTT and PT significantly and inhibited the activities and production of thrombin and FXa. CTXA prolonged in vivo bleeding time and inhibited TNF-α induced PAI-1 production. Furthermore, PAI-1/t-PA ratio was significantly decreased by CTXA. Collectively, these results indicate that CTXA possesses antithrombotic activities and suggest that the current study could provide bases for the development of new anticoagulant agents.

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Acknowledgments

This study was supported by the National Research Foundation of Korea (NRF) funded by the Korea government (MSIP) (Grant No. 2013-067053).

Conflict of interest

The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea

    Hayoung Yoo, Wonhwa Lee, Soyoung Kwak & Jong-Sup Bae

  2. Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea

    Sae-Kwang Ku

  3. Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Wonhwa Lee

  4. Department of Clinical Pathology, Daegu Health College, Daegu, 702-722, Republic of Korea

    Young-Doo Baek

  5. Department of BioMedical Clinical Pathology, Hanlyo University, Gwangyang, 545-704, Republic of Korea

    Byung-Woon Min

  6. College of Pharmacy, Keimyung University, Daegu, 704-701, Republic of Korea

    Gil-Saeng Jeong

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  1. Hayoung Yoo
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  2. Sae-Kwang Ku
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  7. Gil-Saeng Jeong
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Correspondence to Jong-Sup Bae.

Additional information

Hayoung Yoo and Sae-Kwang Ku contributed equally to this work.

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Yoo, H., Ku, SK., Lee, W. et al. Antiplatelet, anticoagulant, and profibrinolytic activities of cudratricusxanthone A. Arch. Pharm. Res. 37, 1069–1078 (2014). https://doi.org/10.1007/s12272-013-0290-4

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  • DOI: https://doi.org/10.1007/s12272-013-0290-4

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