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A new anti-inflammatory triterpene saponin isolated from Anabasis setifera

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Abstract

A bio-guided fractionation of Anabasis setifera Moq. (Chenopodiaceae) for anti-inflammatory activity was carried out using carrageenin rat paw edema model in rats. On the basis of percent edema inhibition after 3 h of carrageenin injection, n-butanol fraction showed promising activity through a significant (p < 0.05) decrease in paw volume by 85.6 % from control using indomethacin as reference standard. Moreover, the n-butanol fraction significantly (p < 0.05) decreased PGE2 and TNF-α in the exudates of rat paw edema. Chemical investigation of n-butanol fraction afforded α-amyrin 3-O-glucopyranoside (1), patuletin 7-O-glucopyranoside (2), myricitrin (3) and a new oleanane triterpene saponin derivative (4), sophradiol 3-O-α-l-1C4-rhamnopyranosyl-(1′′′→4′′)-O-β-d-4C1-galactopyranosyl (1′′→6′)-O-β-d-4C1-glucopyranoside. The structure of the new compound was determined by comprehensive analyses of their 1D and 2D NMR, mass spectral data and comparison with previously known analogs. Only compound 4 revealed a significant (p < 0.05) inhibition of cyclooxygenase 1 and 2 (COX) activities.

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Acknowledgments

This research project was supported by a grant from the Research Centre of the Centre for Female Scientific and Medical Colleges, Deanship of Scientific Research, King Saud University, Saudi Arabia. The authors also are indebted and grateful to Prof. Dr. Olov Sterner and Dr. Karl-Erik Bergquist, faculty of science, Lund university, Sweden, for performing spectral data for all the isolated compounds.

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Authors and Affiliations

  1. Medicinal Chemistry Department, Theodor Bilharz Research Institute (TBRI), Giza, 12411, Egypt

    Allia M. Abdou & Mona A. Mohamed

  2. Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

    Hossam M. Abdallah

  3. Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt

    Hossam M. Abdallah & Ghada A. Fawzy

  4. Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, 22452, Saudi Arabia

    Ghada A. Fawzy

  5. Department of Pharmacology and Toxicology, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

    Ashraf B. Abdel-Naim

Authors
  1. Allia M. Abdou
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  2. Hossam M. Abdallah
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  3. Mona A. Mohamed
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  4. Ghada A. Fawzy
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  5. Ashraf B. Abdel-Naim
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Correspondence to Mona A. Mohamed.

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Abdou, A.M., Abdallah, H.M., Mohamed, M.A. et al. A new anti-inflammatory triterpene saponin isolated from Anabasis setifera . Arch. Pharm. Res. 36, 715–722 (2013). https://doi.org/10.1007/s12272-013-0075-9

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  • DOI: https://doi.org/10.1007/s12272-013-0075-9

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