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miR-7641 modulates the expression of CXCL1 during endothelial differentiation derived from human embryonic stem cells

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Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNAs that negatively regulate gene expression through binding to 3′ untranslated region. We identified and characterized the novel miRNA, miR-7641, in human mesenchymal stem cells. The expression of miR-7641 was downregulated during differentiation from human embryonic stem cells to endothelial cells. The CXCL1, a member of the CXC chemokine family, is known as promoting neovascularization by binding G-protein coupled receptors and is related to endothelial cells biogenesis such as angiogenesis, and it was predicted as target gene of miR-7641 by computerized analysis and the luciferase reporter assay. The miR-7641 significantly suppressed CXCL1 of transcriptional and post-translational levels. These data suggest that miR-7641 might be related with differentiation of human endothelial cells.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) and was funded by the Ministry of Education, Science, and Technology (2012007471).

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Authors and Affiliations

  1. Department of Pharmacy, College of Pharmacy, CHA University, Yatap, Bundang, Seongnam, 463-836, Gyeonggi, Republic of Korea

    Jung Ki Yoo, Ho Yong Jung & Jin Kyeoung Kim

  2. Dongguk University Research Institute of Biotechnology, Medical Science Research Center, Dongguk University School of Medicine, Goyang, 410-773, Gyeonggi, Republic of Korea

    Chang-Hyun Kim

  3. College of Pharmacy, CHA University, 120 Haeryong-ro, Pocheon, 487-010, Gyeonggi, Republic of Korea

    Woo Sung Son

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  1. Jung Ki Yoo
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  2. Ho Yong Jung
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  3. Chang-Hyun Kim
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  4. Woo Sung Son
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  5. Jin Kyeoung Kim
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Correspondence to Woo Sung Son or Jin Kyeoung Kim.

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J. Ki Yoo and H. Y. Jung contributed equally to this work.

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Yoo, J.K., Jung, H.Y., Kim, CH. et al. miR-7641 modulates the expression of CXCL1 during endothelial differentiation derived from human embryonic stem cells. Arch. Pharm. Res. 36, 353–358 (2013). https://doi.org/10.1007/s12272-013-0067-9

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  • DOI: https://doi.org/10.1007/s12272-013-0067-9

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