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In vivo sedative and gastroprotective activities of Salvia plebeia extract and its composition of polyphenols

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Abstract

Animal experiments were performed to develop Salvia plebeia (Labiatae) as a medicinal herb with sedative and gastroprotective activities; the former activity was measured using a pentobarbital-induced assay and the latter activity was measured in two gastric lesion-induced assays (HCl/EtOH-induced and indomethacin/bethanechol-induced assays) in mice. The MeOH extract and its EtOAc fraction were effective, although the former was less active than the latter. Rosmarinic acid (RA) isolated from S. plebeia was active in the same method at 10 and 20 mg/kg (p.o.). HPLC quantification demonstrated that RA comprised the largest proportion (28.5% of the MeOH extract, 33.0% of EtOAc extract; 4.46% of dry weight) of S. plebeia. The contents of five other compounds were much less than that of RA, although the contents of the three glycosides, 6-hydroxyluteolin 7-O-glucoside (0.28% of dry weight), cynaroside (0.35%) and nepitrin (0.43%) were higher than those of the two aglycones, quercetin (0.024%) and eupatilin (0.058%). The HPLC method was validated in terms of linearity, precision, accuracy and reproducibility. These results suggest that the main polyphenol, RA, plays a major role in the sedative and gastroprotective effects of S. plebeia.

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References

  • Beer, D., Joubert, E., Malherbe, C. J., and Brand, D. J., Use of countercurrent chromatography during isolation of 6-hydroxyluteolin-7-O-β-glucoside, a major antioxidant of Athrixia phylicoides. J. Chromatogr. A, 1218, 6179–6186 (2011).

    Article  PubMed  Google Scholar 

  • Behrman, S.W., Management of complicated peptic ulcer disease. Arch. Surg., 140, 201–208 (2005).

    Article  PubMed  Google Scholar 

  • Chapado, L., Linares-Palomino, P. J., Salido, S., Altarejos, J., Rosado, J. A., and Salido, G. M., Synthesis and evaluation of the platelet antiaggregant properties of phenolic antioxidants structurally related to rosmarinic acid. Bioorg. Chem., 38, 108–114 (2010).

    Article  PubMed  CAS  Google Scholar 

  • Coleta, M., Campos, M. G., Cotrim, M. D., Lima, T. C. M., and Cunha, A. P., Assessment of luteolin (3,4,5,7-tetrahydroxyflavone) neuropharmacological activity. Behav. Brain Res., 189, 75–82 (2008).

    Article  PubMed  CAS  Google Scholar 

  • Demir, S., Yilmaz, M., Koseoglu, M., Akalin, N., Aslan, D., and Aydin, A., Role of free radicals in peptic ulcer and gastritis. Turk. J. Gastroenterol., 14, 39–43 (2003).

    PubMed  Google Scholar 

  • Ersoy, S., Orhan, I., Turan, N. N., Sahan, T. G., and Tosun, A. F., Endothelium-dependent induction of vasorelaxation by Melissa officinalis L. ssp. officinalis in rat isolated thoracic aorta. Phytomedicine, 15, 1087–1092 (2008).

    Article  PubMed  CAS  Google Scholar 

  • Fernández, S., Wasowski, C., Paladini, A. C., and Marder, M., Sedative and sleep-enhancing properties of linarin, a flavonoid-isolated from Valeriana officinalis. Pharmacol. Biochem. Behav., 77, 399–404 (2004).

    Article  PubMed  Google Scholar 

  • Harty, R.F., Ancha, H. R., Xia, Y., Anderson, M., and Jazzar, A., GABAergic mechanisms of gastroprotection in the rat: role of sensory neurons, prostaglandins, and nitric oxide. Dig. Dis. Sci., 49, 1875–1881 (2004).

    Article  PubMed  CAS  Google Scholar 

  • Huang, N., Hauck, C., Yum, M. Y., Rizshisky, L., Widrlechner, M. P., McCoy, J. A., Murphy, P. A., Dixon, P. M., Nikolau, B. J., and Birt, D. F., Rosmarinicacid in Prunella vulgaris ethanol extract inhibits lipopolysaccharide-induced prostaglandin E2 and nitric oxide in RAW264.7 mouse macrophages. J. Agric. Food Chem., 25, 10579–10589 (2009).

    Article  Google Scholar 

  • Jäger, A.K., Krydsfeldt, K., and Rasmussen, H. B., Bioassay-guided isolation of apigenin with GABA-benzodiazepine activity from Tanacetum parthenium. Phytother. Res., 23, 1642–1644 (2009).

    Article  PubMed  Google Scholar 

  • Jin, X., Lu, Y., Wei, D., and Wang, Z., Chemical fingerprint and quantitative analysis of Salvia plebeia R.Br. by highperformance liquid chromatography. J. Pharm. Biomed. Anal., 48, 100–104 (2008).

    Article  PubMed  CAS  Google Scholar 

  • Johnston, G.A. R. and Beart, P. M., Flavonoids: some of the wisdom of sage? Brit. J. Pharmacol., 142, 809–810 (2004).

    Article  CAS  Google Scholar 

  • Kavvadias, D., Sand, P., Youdim, K. A., M. Qaiser, M. Z., Rice-Evans, C., Baur, R., Sigel, E., Rausch, W. D., Riederer, P., and Schreier, P., The flavone hispidulin, a benzodiazepine receptor ligand with positive allosteric properties, traverses the blood-brain barrier and exhibits anticonvulsive effects. Brit. J. Pharmacol., 142, 811–820 (2004).

    Article  CAS  Google Scholar 

  • Lee, G.T., Duan, C. H., Lee, J. N., Lee, K. S., Hong, J. T., and Lee, K. K., Phytochemical constituents from Salvia plebeia. Nat. Prod. Sci., 16, 207–210 (2010).

    CAS  Google Scholar 

  • Lewis, D.A., Fields, W. N., and Shaw G. P., A natural flavonoid present in unripe plantain banana pulp (Musa sapientum L. var. paradisiaca) protects the gastric mucosa from aspirin-induced erosions. J. Ethnopharmacol., 65, 283–288 (1999).

    Article  PubMed  CAS  Google Scholar 

  • Lu, Y. and Foo, L.Y., Polyphenolics of Salvia — a review. Phytochemistry, 59, 117–140 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Ly, T.N., Shimoyamada, M., and Yamaguchi, R., Isolation and characterization of rosmarinic acid oligomers in Celastrus hindsii Benth leaves and their antioxidative activity. J. Agric. Food Chem., 54, 3786–3793 (2006).

    Article  PubMed  CAS  Google Scholar 

  • Melo, P.S., Duran, N. D., Hiruma-Lima, C. A., Souza-Brito, A. R. M., and Haun, M., Comparison of the gastroprotective effect of a diterpene lactone isolated from Croton cajucara with its synthetic derivatives. J. Ethnopharmacol., 87, 169–174 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Min, Y.S., Bai, K. L., Yim, S. H., Lee, Y. J., Song, H. J., Kim, J. H., Ham, I, Whang, W. K., and Sohn, U. D., The effect of luteolin-7-O-β-D-glucuronopyranoside on gastritis and esophagitis in rats. Arch. Pharm. Res., 29, 484–489 (2006).

    Article  PubMed  CAS  Google Scholar 

  • Mizui, T. and Dodeuchi M., Effect of polyamines on acidified ethanol induced gastric lesion in rats. Jpn. J. Pharmacol., 33, 939–945 (1983).

    Article  PubMed  CAS  Google Scholar 

  • Najim, R.A. and Saaed, K. K., Drugs acting at the GABAA receptor attenuate ethanol-induced gastric mucosal damage in vitro. Clin. Exp. Pharmacol. Physiol., 30, 495–500 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Nugroho, A., Choi, J., Lee, K., Bachri, M. S., Choi, J. S., Kim, W. B., Lee, K. T., and Park, H. J., Anti-ulcerogenic effect of the caffeoylquinicacid-rich extract from Ligulariastenocephala and HPLC analysis. Biol. Pharm. Bull., 33, 493–497 (2010).

    Article  PubMed  Google Scholar 

  • Osakabe, N., Yasuda, A., Natsume, M., Sanbongi, C., Kato, Y., Osawa, T., and Yoshikawa, T., Rosmarinic acid, a major polyphenolic component of Perilla frutescens, reduces lipopolysaccharide (LPS)-induced liver injury in D-galactosamine (D-GalN)-sensitized mice. Free Radic. Biol. Med., 33, 798–806 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Sanbongi, C., Takanowz, H., Osakabe, N., Sasa, M., Natsume, M., Yanagisawa, R, Inoue, K., Sadakane, K., Ichinose, T., and Yoshikawa, T., Rosmarinic acid in perilla extract inhibits allergic inflammation induced bymite allergen in a mouse model. Clin. Exp. Allergy, 34, 971–977 (2004).

    Article  PubMed  CAS  Google Scholar 

  • Song, H.J., Shin, C. Y., Oh, T. Y., and Sohn, U. D., The protective effect of eupatilin on indomethacin-induced cell damage in cultured feline ileal smooth muscle cells: involvement of HO-1 and ERK. J. Ethnopharmacol., 118, 94–101 (2008).

    Article  PubMed  CAS  Google Scholar 

  • Yesilada, E. and Takaishi, Y., A saponin with anti-ulcerogenic effect from the flowers of Spartium junceum. Phytochemistry, 51, 903–908 (1999).

    Article  PubMed  CAS  Google Scholar 

  • Yesilada, E., Gurbuz, I., Bedir, E., Tatli, I., and Khan, I. A., Isolation of anti-ulcerogenic sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis through bioassayguided fractionation procedures in rats. J. Ethnopharmacol., 95, 213–219 (2004).

    Article  PubMed  CAS  Google Scholar 

  • Yoo, Y.M., Nam, J. H., Kim, M. Y., Choi, J. W., Lee, K. T., and Park, H. J., Analgesic and anti-gastropathic effects of salidroside isolated from Acer tegimentosum Heartwood. The Open Bioact. Comp. J., 2, 28–34 (2009).

    Google Scholar 

  • Yoshikawa, M., Murakami, T., Kishi, A., Kageura, T., and Matsuda, H., Medicinal flowers. III. Marigold. (1): hypoglycemic, gastric emptying inhibitory, and gastroprotective principles and new oleanane-type triterpene oligoglycosides, calendasaponins A, B, C, and D, from Egyptian Calendula officinalis. Chem. Pharm. Bull., 49, 863–870 (2001).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Applied Plant Sciences, Graduate School, Sangji University, Wonju, 220-702, Korea

    Agung Nugroho & Myung-Hoe Kim

  2. Department of Agroindustrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Indonesia, 70712, Indonesia

    Agung Nugroho

  3. College of Pharmacy, Kyungsung University, Busan, 608-736, Korea

    Jongwon Choi

  4. Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, 446-701, Korea

    Nam-In Baek

  5. Department of Pharmaceutical Engineering, Sangji University, Wonju, 220-702, Korea

    Hee-Juhn Park

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  1. Agung Nugroho
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  2. Myung-Hoe Kim
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  3. Jongwon Choi
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  5. Hee-Juhn Park
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Correspondence to Hee-Juhn Park.

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Nugroho, A., Kim, MH., Choi, J. et al. In vivo sedative and gastroprotective activities of Salvia plebeia extract and its composition of polyphenols. Arch. Pharm. Res. 35, 1403–1411 (2012). https://doi.org/10.1007/s12272-012-0810-7

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  • DOI: https://doi.org/10.1007/s12272-012-0810-7

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