Abstract
Bioassay-guided fractionation of the EtOAc-soluble extract of Pueraria lobata based on the inhibition of Aβ-induced toxicity in PC12 cells resulted in the isolation of four known active compounds, genistein (8), biochanin A (9), sissotrin (10), and puerol B (11). Of these, genistein (8) and biochanin A (9) exhibited potent neuroprotective effects with ED50 values of 33.7 and 27.8 μM, respectively. In addition, a new coumestan, 2-(α,α-dimethylallyl)coumestrol (1) was isolated and characterized, but proved to be inactive, as were additional seven known compounds. The structure of new compound 1 was determined using spectroscopic techniques.
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Choi, Yh., Hong, S.S., Shin, Y.S. et al. Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity. Arch. Pharm. Res. 33, 1651–1654 (2010). https://doi.org/10.1007/s12272-010-1014-7
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DOI: https://doi.org/10.1007/s12272-010-1014-7