Abstract
Mushroom-derived polysaccharides (β-glucans) are considered as a valuable biopharmaceutical principle without displaying side effects. Although Tricholoma matsutake is well-known mushroom in Korea, Japan and China, the immunoregulatory roles of T. matsutake-derived polysaccharides were not fully elucidated yet. In this study, we continued to evaluate the immunomodulatory effect of T. matsutake-derived polysaccharide fraction (TmC-2) using functional activation models of macrophages, monocytes and splenic lymphocytes. TmC-2 treatment strongly increased the production of NO and TNF-α. Phagocytic uptake and ROS generation was also up-regulated by TmC-2. Interestingly, TmC-2 stimulated CD29-mediated cell-cell or cell-finbronectin adhesions in monocytes, while CD43-mediated cell adhesion was down-regulated. Interestingly, the enhancement of proliferation and IFN-γ production was striking observed in TmC-2-treated splenic lymphocytes. The activation seemed to be mediated by up-regulating intracellular signaling cascades such as PI3K/Akt and MAPK (ERK and p38) and by the involvement of surface receptors (dectin-1 and TLR-2). Therefore, our results suggest that this TmC-2 from T. matsutake can be developed as a promising immunostimulatory principle, applicable to people with lowered immunomodulatory potentials.
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Bai, X. C., Lu, D., Liu, A. L., Zhang, Z. M., Li, X. M., Zou, Z. P., Zeng, W. S., Cheng, B. L., and Luo, S. Q., Reactive oxygen species stimulates receptor activator of NFkappaB ligand expression in osteoblast. J. Biol. Chem., 280, 17497–17506 (2005).
Byeon, S. E., Chung, J. Y., Lee, Y. G., Kim, B. H., Kim, K. H., and Cho, J. Y., In vitro and in vivo anti-inflammatory effects of taheebo, a water extract from the inner bark of Tabebuia avellanedae. J. Ethnopharmacol., 119, 145–152 (2008).
Cherayil, B. J. and Antos, D., Inducible nitric oxide synthase and Salmonella infection. Microbes Infect., 3, 771–776 (2001).
Cho, J. Y., Baik, K. U., Jung, J. H., and Park, M. H., In vitro anti-inflammatory effects of cynaropicrin, a sesquiterpene lactone, from Saussurea lappa. Eur. J. Pharmacol., 398, 399–407 (2000).
Cho, J. Y., Chain, B. M., Vives, J., Horejsi, V., and Katz, D. R., Regulation of CD43-induced U937 homotypic aggregation. Exp. Cell Res., 290, 155–167 (2003a).
Cho, J. Y., Fox, D. A., Horejsi, V., Sagawa, K., Skubitz, K. M., Katz, D. R., and Chain, B., The functional interactions between CD98, beta1-integrins, and CD147 in the induction of U937 homotypic aggregation. Blood, 98, 374–382 (2001).
Cho, J. Y., Skubitz, K. M., Katz, D. R., and Chain, B. M., CD98-dependent homotypic aggregation is associated with translocation of protein kinase Cdelta and activation of mitogen-activated protein kinases. Exp. Cell Res., 286, 1–11 (2003b).
Choi, J. J., Jin, M., Lee, J. K., Lee, W. Y., Park, Y. I., Han, Y. N., and Kim, S., Control of cytokine gene expression by PG101, a water-soluble extract prepared from Lentinus lepideus. Biochem. Biophys. Res. Commun., 339, 880–887 (2006).
Ebina, T., Antitumor effects of intratumoral injection of Basidiomycetes preparations. Gan To Kagaku Ryoho, 32, 1654–1656 (2005).
Harris, G., KuoLee, R., and Chen, W., Role of Toll-like receptors in health and diseases of gastrointestinal tract. World J. Gastroenterol., 12, 2149–2160 (2006).
Hong, S., Kim, S. H., Rhee, M. H., Kim, A. R., Jung, J. H., Chun, T., Yoo, E. S., and Cho, J. Y., In vitro anti-inflammatory and pro-aggregative effects of a lipid compound, petrocortyne A, from marine sponges. Naunyn Schmiedebergs Arch. Pharmacol., 368, 448–456 (2003).
Jeong, Y. T., Yang, B. K., Jeong, S. C., Kim, S. M., and Song, C. H., Ganoderma applanatum: a promising mushroom for antitumor and immunomodulating activity. Phytother. Res., 22, 614–619 (2008).
Jourd’heuil, D., Morise, Z., Conner, E. M., and Grisham, M. B., Oxidants, transcription factors, and intestinal inflammation. J. Clin. Gastroenterol., 25Suppl 1, S61–72 (1997).
Katsiari, C. G. and Tsokos, G. C., Transcriptional repression of interleukin-2 in human systemic lupus erythematosus. Autoimmun. Rev., 5, 118–121 (2006).
Kim, G. Y., Lee, J. Y., Lee, J. O., Ryu, C. H., Choi, B. T., Jeong, Y. K., Lee, K. W., Jeong, S. C., and Choi, Y. H., Partial characterization and immunostimulatory effect of a novel polysaccharide-protein complex extracted from Phellinus linteus. Biosci. Biotechnol. Biochem., 70, 1218–1226 (2006).
Kim, J. Y., Byeon, S. E., Lee, Y. G., Lee, J. Y., Park, J., Hong, E. K., and Cho, J. Y., Immunostimulatory activities of polysaccharides from liquid culture of pine-mushroom Tricholoma matsutake. J. Microbiol. Biotechnol., 18, 95–103 (2008).
Kodama, N., Komuta, K., and Nanba, H., Effect of Maitake (Grifola frondosa) D-Fraction on the activation of NK cells in cancer patients. J. Med. Food, 6, 371–377 (2003).
Krieg, A. M., Signal transduction induced by immunostimulatory CpG DNA. Springer Semin. Immunopathol., 22, 97–105 (2000).
Larrucea, S., Gonzalez-Rubio, C., Cambronero, R., Ballou, B., Bonay, P., Lopez-Granados, E., Bouvet, P., Fontan, G., Fresno, M., and Lopez-Trascasa, M., Cellular adhesion mediated by factor J, a complement inhibitor. Evidence for nucleolin involvement. J. Biol. Chem., 273, 31718–31725 (1998).
Lee, H. J., Hyun, E. A., Yoon, W. J., Kim, B. H., Rhee, M. H., Kang, H. K., Cho, J. Y., and Yoo, E. S., In vitro antiinflammatory and anti-oxidative effects of Cinnamomum camphora extracts. J. Ethnopharmacol., 103, 208–216 (2006).
Lee, Y. G., Byeon, S. E., Kim, J. Y., Lee, J. Y., Rhee, M. H., Hong, S., Wu, J. C., Lee, H. S., Kim, M. J., Cho, D. H., and Cho, J. Y., Immunomodulatory effect of Hibiscus cannabinus extract on macrophage functions. J. Ethnopharmacol., 113, 62–71 (2007).
Newman, S. L., Macrophages in host defense against Histoplasma capsulatum. Trends Microbiol., 7, 67–71 (1999).
Plenz, G. and Robenek, H., Monocytes/macrophages in atherosclerosis. Eur. Cytokine Netw., 9, 701–703 (1998).
Shiloh, M. U. and Nathan, C. F., Reactive nitrogen intermediates and the pathogenesis of Salmonella and mycobacteria. Curr. Opin. Microbiol., 3, 35–42 (2000).
Stafford, J. L., Neumann, N. F., and Belosevic, M., Macrophage-mediated innate host defense against protozoan parasites. Crit. Rev. Microbiol., 28, 187–248 (2002).
Werling, D. and Jungi, T. W., TOLL-like receptors linking innate and adaptive immune response. Vet. Immunol. Immunopathol., 91, 1–12 (2003).
Zhong, J. and Kyriakis, J. M., Dissection of a signaling pathway by which pathogen-associated molecular patterns recruit the JNK and p38 MAPKs and trigger cytokine release. J. Biol. Chem., 282, 24246–24254 (2007).
Zjawiony, J. K., Biologically active compounds from Aphyllophorales (polypore) fungi. J. Nat. Prod., 67, 300–310 (2004).
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Byeon, S.E., Lee, J., Lee, E. et al. Functional activation of macrophages, monocytes and splenic lymphocytes by polysaccharide fraction from Tricholoma matsutake . Arch. Pharm. Res. 32, 1565–1572 (2009). https://doi.org/10.1007/s12272-009-2108-y
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DOI: https://doi.org/10.1007/s12272-009-2108-y