Abstract
Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC50 value of cimiside E on gastric cancer cells for 24 h was 14.58 µM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 µM) and G2/M phase at higher concentrations (60 and 90 µM). Cimiside E mediated apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways. These findings suggest that cimiside E may be an effective chemopreventive agent against cancer.
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Guo, L.Y., Joo, E.J., Son, K.H. et al. Cimiside E arrests cell cycle and induces cell apoptosis in gastric cancer cells. Arch. Pharm. Res. 32, 1385–1392 (2009). https://doi.org/10.1007/s12272-009-2007-2
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DOI: https://doi.org/10.1007/s12272-009-2007-2