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Triterpenoic acids of Prunella vulgaris var. lilacina and their cytotoxic activities In Vitro

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Abstract

The column chromatographic separation of the MeOH extract from the aerial parts of Prunella vulgaris var. lilacina Nakai led to the isolation of fifteen triterpenoic acids (2–6, 9–13, 16–20), four flavonoids (14, 21–23), four phenolics (7, 8, 15, 24), and a diterpene (1). Their structures were determined by spectroscopic methods to be trans-phytol (1), oleanic acid (2) ursolic acid (3), 2α,3α,19α-trihydroxyurs-12en-28oic acid (4), 2α,3α-dihydroxyurs-12en-28oic acid (5), maslinic acid (6), caffeic acid (7), phydroxy cinnamic acid (8), 2α,3α,19α,23-tetrahydroxyurs-12en-28oic acid (9), 2α,3α,23-trihydroxyurs-12en-28oic acid (10), 2α,3β-dihydroxyurs-12en-28oic acid (11), 2α,3β,24-trihydroxyolea-12en-28oic acid (12), (12R, 13S)-2α,3α,24,trihydroxy-12,13-cyclo-taraxer-14-en-28oic acid (13), quercertin 3-O-β-D-glucopyranoside (14), rosmarinic acid (15), 2α,3α,24-trihydroxyurs-12,20(30)-dien-28oic acid (16), 2α,3α,24-trihydroxyolea-12en-28oic acid (17), 2α,3β,19α,24-tetrahydroxyurs-12en-28oic acid 28-O-Dglucopyranoside (18), 2α,3α,19α,24-tetrahydroxyurs-12en-28oic acid 28-O-D-glucopyranoside (19), prunvuloside A (20), kaempferol 3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopranoside (21), kaempferol 3-O-β-D-glucopyranoside (22), quercertin 3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside (23), and 2-hydroxy-3-(3’,4’-dihydroxyphenly)propanoic acid (24). Compounds 1, 8–12, 17, 21, 23, and 24 were isolated from this plant source for the first time. The isolated compounds were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT15 cells in vitro using the sulforhodamin B bioassay (SRB) method. Compound 3 exhibited moderate cytotoxic activity against A549, SK-OV-3, SK-MEL-2, and HCT15 cells, with ED50 values of 3.71, 3.65, 13.62, and 5.44 μM, respectively.

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Authors and Affiliations

  1. Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea

    Il Kyun Lee, Do Hoon Kim, Seung Young Lee, Kyung Ran Kim, Sang Un Choi, Jong Ki Hong, Jei Hyun Lee, Young Hyun Park & Kang Ro Lee

  2. Korea Research Institute of Chemical Technology, Teajeon, 305-600, Korea

    Sang Un Choi

  3. Pharmaceutical Analysis Laboratory, College of Pharmacy, Kyung Hee University, Seoul, 130-701, Korea

    Jong Ki Hong

  4. College of Oriental Medicine, Dongguk University, Gyeong ju, 780-714, Korea

    Jei Hyun Lee

  5. Department of Food Science and Nutrition, Soonchunhyang University, Asan, 336-745, Korea

    Young Hyun Park

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  1. Il Kyun Lee
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Correspondence to Kang Ro Lee.

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Lee, I.K., Kim, D.H., Lee, S.Y. et al. Triterpenoic acids of Prunella vulgaris var. lilacina and their cytotoxic activities In Vitro . Arch. Pharm. Res. 31, 1578–1583 (2008). https://doi.org/10.1007/s12272-001-2154-6

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  • DOI: https://doi.org/10.1007/s12272-001-2154-6

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