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Continuous conversion of rice starch hydrolysate to 2-keto-D-gluconic acid by Arthrobacter globiformis C224

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Abstract

A continuous conversion process of rice starch hydrolysate to 2-keto-D-gluconic acid (2KGA) by Arthrobacter globiformis C224 was developed. Its feasibility for industrial application was also evaluated. Results showed that the initial cell concentration exceeding 1.25 g/L met the continuous 2KGA production at a stable dilution rate and media composition, while the dilution rate and feeding glucose concentration had a significant effect on 2KGA production performance. The optimal operating parameters were obtained as: 0.090/h of dilution rate and 171.0 g/L of feeding glucose concentration. Under these conditions, the steady state had a produced 2KGA concentration of 124.74 g/L, average volumetric productivity of 11.23 g/L/h, and yield of 0.97 g/g. In conclusion, continuous 2KGA production by the A. globiformis C224 strain would be a superior industrial process for the production of 2KGA in terms of its high 2KGA productivity and yield.

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Authors and Affiliations

  1. College of Life Science, Hebei Normal University, Shijiazhuang, 050-016, China

    Wen-Hua Teng, Wen-Jing Sun & Jing-Ze Liu

  2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212-013, China

    Wen-Jing Sun, Feng-Jie Cui, Jing-Ya Qian, Liang Wang & Xiang-Hui Qi

  3. Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production, Dexing, 334-221, China

    Wen-Jing Sun, Bin Yu, Feng-Jie Cui, Jing-Ya Qian, Liang Wang & Xiang-Hui Qi

  4. Parchn Sodium Isovitamin C Co., Ltd., Dexing, 334-221, China

    Bin Yu

  5. Jiangxi OAI Joint Research Institute, Nanchang University, Jiangxi, 330-047, China

    Hua Wei

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  1. Wen-Hua Teng
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  2. Wen-Jing Sun
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  3. Bin Yu
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  9. Hua Wei
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Correspondence to Wen-Jing Sun or Feng-Jie Cui.

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These authors are the co-first authors and have the equal contributions.

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Teng, WH., Sun, WJ., Yu, B. et al. Continuous conversion of rice starch hydrolysate to 2-keto-D-gluconic acid by Arthrobacter globiformis C224. Biotechnol Bioproc E 18, 709–714 (2013). https://doi.org/10.1007/s12257-012-0831-6

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  • DOI: https://doi.org/10.1007/s12257-012-0831-6

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