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PDEA-coated magnetic nanoparticles for gene delivery to Hep G2 cells

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  • Medical Biotechnology
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Abstract

Poly(2-(diethylamino)ethyl methacrylate) coated magnetic nanoparticles (PDEA-MNPs) were synthesized as a new gene nanocarrier to delivery plasmids (pEGFPN1 and pRL-TK) into human hepatoma (Hep G2) cells. The PDEA-MNPs shows the pH-sensitive property. These nanoparticles are positively charged at acidic pH and negatively charged at neutral or alkaline pH. The PDEAMNPs exhibited a low cytotoxicity in Hep G2 cells. PDEA-MNPs could bind and protect DNA from DNase I degradation. The transfection study demonstrated that the PDEA-MNPs could carry plasmid into Hep G2 cells and exhibited a high gene transfection efficiency. These results indicated that the novel magnetic nanoparticles could enhance gene transfection in vitro and hold the potential to be a promising non-viral nanodevice.

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Authors and Affiliations

  1. Dezhou Institute of Advanced Materials, Dezhou University, Dezhou, 253-023, China

    Hanwen Sun, Xinjun Zhu, Lianying Zhang, Xiangling Gu, Jinghe Wang, Jing Li & Yancong Zhang

  2. Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin, 300-071, China

    Hanwen Sun

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  1. Hanwen Sun
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  2. Xinjun Zhu
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  3. Lianying Zhang
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  4. Xiangling Gu
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Correspondence to Hanwen Sun.

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Sun, H., Zhu, X., Zhang, L. et al. PDEA-coated magnetic nanoparticles for gene delivery to Hep G2 cells. Biotechnol Bioproc E 18, 648–654 (2013). https://doi.org/10.1007/s12257-012-0720-z

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  • DOI: https://doi.org/10.1007/s12257-012-0720-z

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