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Optimization and monitoring of water soluble substrate for synthesis of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657)

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Abstract

Biosurfactants are amphipathic molecules, consisting of hydrophobic and hydrophilic domains, which can partition at the interfaces between different fluid phases, such as oil/water or water/air interfaces. Mannosylerithritol lipids (MELs) are a type of glycolipid. The yeast Candida (Pseudozyma) antarctica (ATCC 32657) secretes an extracellular MEL, with biosurfactant properties, when grown on a water soluble and insoluble substrate, such as glycerine or oils. Value added MEL was biologically synthesized, by using two different types of honey as natural and newer water soluble carbon source. Those types of honey as a carbon source were optimized at different concentration of total carbohydrate present in the honey. Further optimized substrate concentration of honey was monitored for 15 days, for studies of MEL yield and cell growth concentration per day on shake flask batch culture. Surfactant properties, such as surface tension, interfacial tension, foaming, emulsification and wetting of MEL, were observed. Produced MEL was confirmed by thin layer chromatography (TLC), Fourier Transform Infrared (FTIR) spectra, and 1H Nuclear Magnetic Resonance (NMR) techniques.

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Authors and Affiliations

  1. Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; Formerly UDCT/UICT), Mumbai, 400-019, India

    Akash Bhangale, Sushant Wadekar & Amit Pratap

  2. DBT-ICT Centre for Energy Biosciences, Department of Chemical Engineering, Institute of Chemical Technology (University under Section 3 of UGC Act 1956; Formerly UDCT/UICT), Mumbai, 400-019, India

    Sandeep Kale

Authors
  1. Akash Bhangale
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  2. Sushant Wadekar
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  3. Sandeep Kale
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  4. Amit Pratap
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Correspondence to Amit Pratap.

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Bhangale, A., Wadekar, S., Kale, S. et al. Optimization and monitoring of water soluble substrate for synthesis of mannosylerythritol lipids by Pseudozyma antarctica (ATCC 32657). Biotechnol Bioproc E 18, 679–685 (2013). https://doi.org/10.1007/s12257-012-0647-4

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  • DOI: https://doi.org/10.1007/s12257-012-0647-4

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