Abstract
Alopecia is not life threatening, but patients who undergo alopecia often experience severe mental stress. In addition, the number of individuals afflicted by alopecia has been increasing steadily. The most effective treatment of alopecia developed to date is auto hair transplantation. To overcome the limitations associated with current therapies for the treatment of alopecia, many researchers have attempted to revive hair follicles by in vitro culture of hair follicle cells and subsequent implantation in the treatment area. Previously, we demonstrated that umbilical cord-derived mesenchymal stem cells (UC-MSCs) could be isolated and expanded successfully from the Wharton’s Jelly. Cultureexpanded UC-MSCs formed aggregates similar to native dermal papilla (DP) in special media (DPFM) and reconstructed dermal papilla like tissues (DPLTs) could induce new hair follicles. The purpose of the present study was to optimize the reconstruction of DPLTs. As in the case of MSCs, when compared to differentiated cells, DPLTs require an additional step to induce differentiation into dermal papilla cells. However, it is necessary to use hepatocyte growth factor (HGF) in the differentiation step, which is relatively expensive. To reduce the expenses associated with cell therapy using MSCs, it is necessary to optimize this differentiation step. To accomplish this, we evaluated the effects of cell inoculation density and growth factors during differentiation.
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References
Li, L. and R. M. Hoffman (1995) The feasibility of targeted selective gene therapy of the hair follicle. Nat. Med. 1: 705–706.
Yoo, B. Y., Y. H. Shin, H. H. Yoon, Y. J. Kim, K. Y. Song, S. J. Hwang, and J. K. Park (2007) Improved isolation of outer root sheath cells from human hair folli cles and their proliferation behavior under serum-free condition. Biotechnol. Bioproccess Eng. 12: 54–59.
Commo, S., O. Gaillard, and B. A. Bernard (2000) The human hair follicle contains two distinct K19 positive compartments in the outer root sheath: a unifying hypothesis for stem cell reservoir? Differentiation 66: 157–164.
Seo, Y.-K., D.-H. Lee, Y.-H. Shin, B.-Y. Yoo, K.-M. Lee, K.-Y. Song, S.-J. Seo, S.-J. Hwang, Y.-J. Kim, E. -K. Yang, C.-S. Park, I.-S. Chang, and J.-K. Park (2003) Development of isolation and cultivation method for outer root sheath cells from human hair follicle and construction of bioartificial skin. Biotechnol. Bioproccess Eng. 8: 151–157.
McElwee, K. J., S. Kissling, E. Wenzel, A. Huth, and R. Hoffman (2003) Cultured peribulbar dermal sheath cells can induce hair follicle development and contribute to the dermal sheath and dermal papilla. J. Invest. Dermatol. 121: 1267–1275.
Elliott, K., T. J. Stephenson, and A. G. Messenger (1999) Differences in hair follicle dermal papilla volume are due to extracellular matrix volume and cell number: implications for the control of hair follicle size and androgen responses. J. Invest. Dermatol. 113: 873–877.
Katsuoka, K., C. Mauch, H. Schell, O. P. Hornstein, and Th. Krieg (1988) Collagen-type synthesis in human-hair papilla cells in culture. Arch. Dermatol. Res. 280: 140–144.
Ozeki, M. and Y. Tabata (2003) In vivo promoted growth of mice hair follicles by the controlled release of growth factors. Biomaterials 24: 2387–2394.
Lee, Y. R. (2001) Hepatocyte growth factor (HGF) activator expressed in hair follicles is involved in in vitro HGF-dependent hair follicle elongation. J. Dermatol. Sci. 25: 156–163.
Shimaoka, S., R. Imai, and H. Ogawa (1994) Dermal papilla cells express hepatocyte growth factor. J. Dermatol. Sci. 7: S79–83.
Jindo, T., R. Tsuboi, R. Imai, K. Takamori, J. S. Rubin, and H. Ogawa (1995) The effect of hepatocyte growth factor/scatter factor on human hair follicle growth. J. Dermatol. Sci. 10: 229–232.
Yamazaki, M., R. Tsuboi, Y. R. Lee, K. Ishidoh, S. Mitsui, and H. Ogawa (1999) Hair cycle-dependent expression of hepatocyte growth factor (HGF) activator, other proteinases, and proteinase inhibitors correlates with the expression of HGF in rat hair follicles. J. Invest. Dermatol. Symp. Proc. 4: 312–315.
Peus, D. and M. R. Pittelkow (1996) Growth factors in hair organ development and the hair growth cycle. Dermatol. Clin. 14: 559–572.
Shimaoka, S., R. Tsuboi, T. Jindo, R. Imai, K. Takamori, J. S. Rubin, and H. Ogawa (1995) Hepatocyte growth factor/scatter factor expressed in follicular papilla cells stimulates human hair growth in vitro. J. Cell Physiol. 165: 333–338.
Jindo, T., R. Tsuboi, R. Imai, K. Takamori, J. S. Rubin, and H. Ogawa (1994) Hepatocyte growth factor/scatter factor stimulates hair growth of mouse vibrissae in organ culture. J. Invest. Dermatol. 103: 306–309.
Jindo, T., R. Tsuboi, K. Takamori, and H. Ogawa (1998) Local injection of hepatocyte growth factor/scatter factor (HGF/SF) alters cyclic growth of murine hair follicles. J. Invest. Dermatol. 110: 338–342.
Katsuoka, K., H. Schell, B. Wessel, and O. P. Hornstein (1987) Effects of epidermal growth factor, fibroblast growth factor, minoxidil, and hydrocortisone on growth kinetics in human hair bulb papilla cells and root sheath fibroblasts cultured in vitro. Arch. Dermatol. Res. 279: 247–250.
Lu, Z. F., S. Q. Cai, J. J. Wu, and M. Zheng (2006) Biological characterization of cultured dermal papilla cells and hair follicle regeneration in vitro and in vivo. Chin. Med. J. (Engl) 119: 275–281.
Chiu, H. C., C. H. Chang, J. S. Chen, and S. H. Jee (1996) Human hair follicle dermal papilla cell, dermal sheath cell, and interstitial dermal fibroblast characteristics. J. Formos. Med. Assoc. 95: 667–674.
Osada, A., T. Iwabuchi, J. Kishimoto, T. S. Hamazaki, and H. Okochi (2007) Long-term culture of mouse vibrissal dermal papilla cells and de novo hair follicle induction. Tissue Eng. 13: 975–982.
Limat, A., T. Hunziker, E. R. Waelti, S. P. Inaebnit, U. Wiesmann, and L. R. Braathen (1993) Soluble factors from human hair papilla cells and dermal fibroblasts dramatically increase the clonal growth of outer root sheath cells. Arch. Dermatol. Res. 285: 205–210.
Yano, K., L. F. Brown, and M. Detmar (2001) Control of hair growth and follicle size by VEGF-mediated angiogenesis. J. Clin. Invest. 107: 409–417.
Yoo, H. G., I. Y. Chang, H. K. Pyo, Y. J. Kang, S. H. Lee, O. S. Kwon, K. H. Cho, H. Kwang, H. C. Eun, and K. H. Kim (2007) The additive effects of minoxidil and retinol on human hair growth in vitro. Biol. Pharm. Bull 30: 21–26.
Rho, S. S., S. J. Park, S. L. Hwang, M. H. Lee, C. D. Kim, I. H. Lee, S. Y. Chang, and M. J. Rang (2005) The hair growth promoting effect of Asiasari radix extract and its molecular regulation. J. Dermatol. Sci. 38: 89–97.
Charveron, M. and S. Lachgar (1998) Hair growth: VEGF (vascular endothelial growth factor) and fibroblasts of hair dermal papilla cells. Ann. Dermatol. Vener. 125: S9–S11.
Yoo, B. Y., Y. H. Shi, K. Y. Song, H. H. Yoon, and J. K. Park (2009) Evaluation of human umbilical cordderived mesenchymal stem cells on in vivo hair inducing activity. Tissue Eng. Regen. Med. 6: 15–22.
Method for the preparation of a dermal papilla tissue having hair follicle inductive potency. Pct kr 2005 004040: (2005)
Stenn, K. S. and R. Paus (2001) Controls of hair follicle cycling. Physiol. Rev. 81: 449–494.
Argyris, T. (1976) Kinetics of epidermal production during epidermal regeneration following abrasion in mice. Am. J. Pathol. 83: 329–340.
Ansel, J. C., C. A. Armstrong, I. Song, K. L. Quinlan, J. E. Olerud, S. W. Caughman, and N. W. Bunnett (1997) Interactions of the skin and nervous system. J. Investig. Dermatol. Symp. Proc. 2: 23–26.
Arase, S., Y. Sadamoto, S. Katoh, Y. Urano, and K. Takeda (1990) Co-culture of human hair follicles and dermal papillae in a collagen matrix. J. Dermatol. 17: 667–676.
Green, M. R. and J. R. Couchman (1984) Distribution of epidermal growth factor receptors in rat tissues during embryonic skin development, hair formation, and the adult hair growth cycle. J. Invest. Dermatol. 83: 118–123.
du Cros, D. L., K. Isaacs, and G. P. Moore (1992) Localization of epidermal growth factor immunoreactivity in sheep skin during wool follicle development. J. Invest. Dermatol. 98: 109–115.
Nanney, L. B., C. M. Stoscheck, L. E. Jr. King, R. A. Underwood, and K. A. Holbrook (1990) Immunolocalization of epidermal growth factor receptors in normal developing human skin. J. Invest. Dermatol. 94: 742–748.
Moore, G. P., B. A. Panaretto, and N. B. Carter (1985) Epidermal hyperplasia and wool follicle regression in sheep infused with epidermal growth factor. J. Invest. Dermatol. 84: 172–175.
Donovan, M. J., R. Hahn, L. Tessarolloand, B. L. Hempstead (1996) Identification of an essential nonneuronal function of neurotrophin 3 in mammalian cardiac development. Nat. Genet. 14: 210–213.
Ojeda, S. R., C. Romero, V. Tapia, and G. A. Dissen (2000) Neurotrophic and cell-cell dependent control of early follicular development. Mol.Cell Endocrinol. 163: 67–71.
Thornton, M. J., K. Hamada, A. G. Messenger, V. A. Randall (1998) Androgen-dependent beard dermal papilla cells secrete autocrine growth factor(s) in response to testosterone unlike scalp cells. J. Invest. Dermatol. 111: 727–732.
Chuang, Y. H., D. Dean, J. Allen, R. Dawber, and F. Wojnarowskaf (2003) Comparison between the expression of basement membrane zone antigens of human interfollicular epidermis and anagen hair follicle using indirect immunofluorescence. Br. J. Dermatol. 149: 274–281.
Jahoda, C. A. (1992) Induction of follicle formation and hair growth by vibrissa dermal papillae implanted into rat ear wounds: vibrissa-type fibres are specified. Development 115: 1103–1109.
Reynolds, A. J. and C. A. Jahoda (1992) Cultured dermal papilla cells induce follicle formation and hair growth by transdifferentiation of an adult epidermis. Development 115: 587–593.
Reynolds, A. J., C. Lawrence, P. B. Cserhalmi-Friedman, A. M. Christiano, and C. A. B. Jahoda (1999) Transgender induction of hair follicles. Nature 402: 33–34.
Kishimoto, J., R. Ehama, L. Wu, S. Jiang, N. Jiang, and R. E. Burgeson (1999) Selective activation of the versican promoter by epithelial-mesenchymal interactions during hair follicle development. Proc. Natl. Acad. Sci. USA 96: 7336–7341.
Hashimoto, T., T. Kazama, M. Ito, K. Urano, Y. Katakai, N. Yamaguchi, and Y. Ueymamy (2000) Histologic and cell kinetic studies of hair loss and subsequent recovery process of human scalp hair follicles grafted onto severe combined immunodeficient mice. J. Invest. Dermatol. 115: 200–206.
Ehama, R., Y. Ishimatsu-Tsuji, S. Iriyama, R. Ideta, T. Soma, K. Yano, C. Kawasaki, S. Suzuki, Y. Shirakata, K. Hashimoto, and J. Kishimoto (2007) Hair follicle regeneration using grafted rodent and human cells. J. Invest. Dermatol. 127: 2106–2115
Qiao, J., A. Turetsky, P. Kemp, and J. Teumer (2008) Hair morphogenesis in vitro: formation of hair structures suitable for implantation. Regen. Med. 3: 683–692.
Lin, C. M., Y. C. Ji, H. Keng, X. N. Cai, and J. K. Zhang (2008) Microencapsulated human hair dermal papilla cells: a substitute for dermal papilla? Arch. Dermatol. Res. 300: 531–535.
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Yoo, BY., Shin, YH., Yoon, HH. et al. Optimization of the reconstruction of dermal papilla like tissues employing umbilical cord mesenchymal stem cells. Biotechnol Bioproc E 15, 182–190 (2010). https://doi.org/10.1007/s12257-009-3050-z
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DOI: https://doi.org/10.1007/s12257-009-3050-z