Skip to main content
SpringerLink
Log in

Accumulation of sesquiterpenes and polysaccharides in cells of zedoary (Curcuma zedoaria Roscoe) cultured in a 10 L bioreactor

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

We developed a cell suspension culture system for zedoary (Curcuma zedoaria Roscoe), using 100 g fresh weight inoculum in a batch culture. The maximum cell biomass of 68.46 g/L fresh weight was obtained after 14 days of culture in a 10 L bioreactor with a pitch-blade impeller maintained at an agitation speed of 150 rpm and an aeration rate of 2.5 L/min. The accumulation of sesquiterpenes and polysaccharide in zedoary cells from 2 to 18 days was measured by HPLC and a phenol-sulfuric acid assay, respectively. The total polysaccharide concentration increased between 2 to 10 days of culture and reached a maximum value of 6.55%. HPLC revealed several eluted peaks of sesquiterpenes, which increased in amplitude from days 2 to 10. Furthermore, our results indicated that biotransformation occurred in the cell suspension, transforming certain sesquiterpenes into other types during culture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Maeda, H., N. Sunagane, and K. Kubota (1984) Pharmacological effects of the powder from Curcuma zedoaria on the gastrointestinal tract of experimental animals. Yakugaku Zasshi 104: 640–643.

    CAS  Google Scholar 

  2. Ruby, A. J., G. Kuttan, K. D. Babu, K. N. Rajasekharan, and R. Kuttan (1995) Anti-tumor and antioxidant activity of natural curcuminoids. Cancer Lett. 94: 79–83.

    Article  CAS  Google Scholar 

  3. Limtrakul, P., S. Lipigorngoson, O. Namwong, A. Apisariyakul, and F. W. Dunn (1997) Inhibitory effect of dietary curcumin on skin carcinogenesis in mice. Cancer Lett. 116: 197–203.

    Article  CAS  Google Scholar 

  4. Srivastava, K. C., A. Bordia, and S. K. Verma (1995) Curcumin, a major component of food spice turmeric, inhibits aggregation and alters eicosanoid metabolism in human blood platelets. Prostaglandins Leukot Essent Fatty Acids 52: 223–227.

    Article  CAS  Google Scholar 

  5. Kim, K. I., J. W. Kim, B. S. Hong, D. H. Shin, H. Y. Cho, H. K. Kim, and H. C. Yang (2000) Antitumor, genotoxicity and anticlastogenic activities of polysaccharide from Curcuma zedoaria. Mol. Cells 10: 392–398.

    CAS  Google Scholar 

  6. Kim, K. I., K. S. Shin, W. J. Jun, B. S. Hong, D. H. Shin, Y. C. Hong, H. I. Chang, S. M. Yoo, and H. C. Yang (2001) Effects of polysaccharides from rhizomes of Curcuma zedoaria on macrophage functions. Biosci. Biotechnol. Biochem. 65: 2369–2377.

    Article  CAS  Google Scholar 

  7. Furita, Y. and M. Tabata (1987) Secondary metabolites from plant cells: pharmaceutical application and progress in commercial production. pp. 169–185. In: C. E. Green, D. A. Somers, W. P. Hackett, and D.D. Biesboer (eds.). Plant Tissue and Cell Culture. Alan R Liss, NY, USA.

  8. Furita, Y. (1988) Shikonin: Production by plant (Lithospermum erythrorhizon) cell cultures. pp. 225–236. In: Y. P. S. Bajaj (ed.). Biotechnology in Agriculture and Forestry: Medicinal and Aromatic Plants. Springer-Verlag, Berlin, Germany.

    Google Scholar 

  9. Ushiyama, K. (1991) Large scale culture of ginseng. pp. 92–98. In: A. Komamine, M. Misawa, and F. DiCosmo (eds.). Plant Cell Culture in Japan. CMC, Tokyo, Japan.

    Google Scholar 

  10. Schlatmann, J. E., A. M. Nuutila, W. M. Van Gulik, H. J. G. Ten Hoopen, R. Verpoorte, and J. J. Heijnen (1993) Scale-up of ajmalicine production by plant cell cultures of Catharanthus roseus. Biotechnol. Bioengin. 41: 253–262.

    Article  CAS  Google Scholar 

  11. Sato, K., M. Nakayama, and J. I. Shigeta (1996) Culturing conditions affecting the production of anthocyanin in suspended cell cultures of strawberry. Plant Sci. 113: 91–98.

    Article  CAS  Google Scholar 

  12. Seki, M., C. Ohzora, M. Takeda, and S. Furusaki (1997) Taxol (Paclitaxel) production using free and immobilized cells of Taxus cuspidata. Biotechnol. Bioeng. 53: 214–219.

    Article  CAS  Google Scholar 

  13. Noble, R. L. (1990) The discovery of the vinca alkaloids-chemotherapeutic agents against cancer. Biochem. Cell Biol. 68: 1344–1351.

    Article  CAS  Google Scholar 

  14. Loc, N. H., D. T. Duc, T. H. Kwon, and M. S. Yang (2005) Micropropagation of zedoary (C. zedoaria Roscoe)-a valuable medicinal plant. Plant Cell Tiss. Organ Cult. 81: 119–122.

    Article  CAS  Google Scholar 

  15. Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15: 473–497.

    Article  CAS  Google Scholar 

  16. Loc, N. H., T. T. T. Ha, and Y. Hirata (2006) Effect of several factors on cell biomass production of zedoary (C. zedoaria Roscoe) in bioreactor. Vietnamese J. Biotechnol. 4: 213–220.

    Google Scholar 

  17. Jang, M. K., D. H. Sohn, and J. H. Ryu (2004). A curcuminoid and two sesquiterpenoids from Curcuma zedoaria as inhibitors of nitric oxide synthesis in activated macrophages. Arch. Pharm. Res. 27:1220–1225.

    Article  CAS  Google Scholar 

  18. Sun, Y. L., J. Tang, X. H. Gu, and D. Y. Li (2005) Watersoluble polysaccharides from Angelica sinensis (Oliv.) Diels: Preparation, characterization, and bioacitivity. Int. J. Biol. Macromol. 36: 283–289.

    Article  CAS  Google Scholar 

  19. Chaplin, M. F. and J. F. Kennedy (1994) Carbohydrate Analysis. A Practical Approach. 2nd ed., pp. 143–204. Oxford University Press, Oxford, UK.

    Google Scholar 

  20. Li, X. L. and A. G. Zhou (2007) Preparation of polysaccharides from Acanthopanax senticosus and its inhibition against irradiation-induced injury of rat. Carbohydr. Polymers 67: 219–226.

    Article  CAS  Google Scholar 

  21. Zhao, J. and R. Verpoorte (2007) Manipulating indole alkaloid production by Catharanthus roseus cell cultures in bioreactors: From biochemical processing to metabolic engineering. Phytochem. Rev. 6: 435–457.

    Article  CAS  Google Scholar 

  22. Eibl, R. and D. Eibl (2008) Design of bioreactors suitable for plant cell and tissue cultures. Phytochem. Rev. 7: 593–598.

    Article  CAS  Google Scholar 

  23. Ah, T., N. Ma, and R. Zaiton (2004) Multiplication of oil palm suspension cultures in a bench-top (2L) bioreactor. J. Oil Palm Research 16: 44–49.

    Google Scholar 

  24. Pavlov, A. I., M. I. Georgiev, I. N. Panchev, and M. P. Ilieva (2005) Optimization of rosmarinic acid production by Lavandula vera MM plant cell suspension in a laboratory bioreactor. Biotechnol. Prog. 21: 394–396.

    Article  CAS  Google Scholar 

  25. Mantzouridou, F., T. Roukas, and P. Kotzekidou (2002) Effect of the aeration rate and agitation speed on β-carotene production and morphology of Blakeslea trispora in a stirred tank reactor: Mathematical modeling. Biochem. Engin. J. 10: 123–135.

    Article  CAS  Google Scholar 

  26. Chattoppadhyay, S., A. K. Srivastava, S. S. Bhojwani, and V. S. Bisaria (2002) Production of podophyllotoxin by plant cell cultures of Podophyllum hexandrum in bioreactor. J. Biosci. Bioengin. 93: 215–220.

    Article  Google Scholar 

  27. Nahar, L. and S. D. Sarker (2007) Phytochemistry of the genus Curcuma. pp. 71–106. In: P.N. Ravindran, K.N. Babu, and K. Sivaraman (eds.). Turmeric-The genus Curcuma. CRC Press, Taylor and Francis Group, USA.

    Google Scholar 

  28. Sakui, N., M. Kuroyanagi, Y. Ishitobi, M. Sato, and A. Ueno (1992) Biotransformation of sesquiterpenes by cultured cells of Curcuma zedoaria. Phytochem. 31: 143–147.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Resources, Environment and Biotechnology, Hue University, Hue, Vietnam

    Nguyen Hoang Loc, Doan Huu Nhat Binh, Truong Thi Bich Phuong & Tae-Geum Kim

  2. College of Education, Danang University, Danang, Vietnam

    Vo Chau Tuan

  3. Division of Biological Sciences and Research Center for Bioactive Materials, Chonbuk National University, Chonbuk, 561-756, Korea

    Moon-Sik Yang

Authors
  1. Nguyen Hoang Loc
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vo Chau Tuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Doan Huu Nhat Binh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Truong Thi Bich Phuong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tae-Geum Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Moon-Sik Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Moon-Sik Yang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Loc, N.H., Tuan, V.C., Binh, D.H.N. et al. Accumulation of sesquiterpenes and polysaccharides in cells of zedoary (Curcuma zedoaria Roscoe) cultured in a 10 L bioreactor. Biotechnol Bioproc E 14, 619–624 (2009). https://doi.org/10.1007/s12257-009-0013-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-009-0013-3

Keywords

Search

Navigation