Skip to main content
SpringerLink
Log in

Comparative evaluation of antioxidant, nitrite scavenging, and antitumor effects of Antrodia camphorata extract

  • Articles
  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

The effects of decoctions prepared from Antrodia camphorata on antioxidant, nitrite scavenging, and antitumor activities were investigated. Glutathione (GSH) production was 18.2 μM/g of rat liver under the influence of a methanol extract, a result similar to with the effect of silymarin administration. GSH peroxidase activity was about 2.0-fold higher than with silymarin administration. Superoxide dismutase activity was 18.9 U/mg protein, about 2.5-fold higher than the control group. The hot water extract (1000 μg/mL) showed the highest nitrite scavenging activity at 98.1%, similar to those observed with BHA and vitamin E. Among a variety of human cancer cell lines, when the concentration of hot water extract was increased from 31 to 500 μg/mL, the viability of Hep3B cell was decreased from 100 to 60.7%. On the other hand, in the case of methanol extract, it was decreased from 98.4 to 10.0%. These results supported the conclusion that the antioxidant, nitrite scavenging, and antitumor activities of this A. camphorata methanol extract indicate a potential source for the development of various health supplements and pharmaceutical and nutraceutical applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Halliwell, B. and J. M. C. Gutteridge (1984) Oxygen toxicity, oxygen radicals, transition metals, and disease. Biochem. J. 219: 1–8.

    CAS  Google Scholar 

  2. Kim, I. S., H. S. Yun, S. H. Kwak, and I. N. Jin (2007) The physiological role of CPR1 in Saccharomyces cerevisiae KNU5377 against menadione stress by proteomics. J. Microbiol. 45: 326–332.

    CAS  Google Scholar 

  3. Lorenzen, K. and T. Anke (1998) Basidiomycetes as a source for new bioactive natural products. Curr. Org. Chem. 2: 329–364.

    CAS  Google Scholar 

  4. Wasser, S. P. and A. L. Weis (1999) Medicinal properties of substances occurring in higher Basidiomycetes mushrooms: current perspectives. Int. J. Med. Mush. 1: 31–62.

    CAS  Google Scholar 

  5. Kim, M. J., H. N. Seo, T. S. Hwang, S. H. Lee, and D. H. Park (2008) Characterization of exopolysaccharide produced by Weissella hellenica SK kimchi isolated from Kimchi. J. Microbiol. 46: 535–541.

    Article  CAS  Google Scholar 

  6. Shinozaki-Kuwahara, N., K. Takada, and M. Hirasawa (2008) Sequence and phylogenetic analyses of novel glucosyltransferase genes of mutans Streptococci I solated from pig oral cavity. J. Microbiol. 46: 202–208.

    Article  CAS  Google Scholar 

  7. Ooi, V. E. C. and F. Liu (1999) A review of pharmacological activities of mushroom polysaccharides. Int. J. Med. Mush. 1: 195–206.

    CAS  Google Scholar 

  8. Czop, J. K. and J. Kay (1991) Isolation and characterization of β-glucan receptors on human mononuclear phagocytes. J. Exp. Med. 173: 1511–1520.

    Article  CAS  Google Scholar 

  9. Yanaki, T., W. Ito, and T. Kojima (1981) Ultrasonic degradation of schizophyllan, an antitumor polysaccharide produced by Schizophyllum commune FRIES. Carbohydr. Res. 89: 121–135.

    Article  Google Scholar 

  10. Choi, D. B., S. Y. Kang, Y. H. Song, K. H. Kwun, K. J. Jeong, and W. S. Cha (2005) Exopolysaccharide production in liquid culture of Pleurotus ferulae. J. Microbiol. Biotechnol. 15: 368–375.

    CAS  Google Scholar 

  11. Choi, D. B., H. G. Nam, and W. S. Cha (2006) Studies on cultivation and biological activities of Pleurotus nebrodensis Inzenga. Kor. J. Chem. Eng. 23: 241–246.

    Article  CAS  Google Scholar 

  12. Cha, W. S., D. B. Choi, and S. H. Kang (2004) Optimization of culture media for solid-state culture of Pleurotus ferulae. Biotechnol. Bioprocess Eng. 9: 369–373.

    Article  CAS  Google Scholar 

  13. Shin, S. E., W. S. Cha, and S. H. Kang (2003) A study on the mycelial growth of Lentinus lepideus in liquid culture. Kor. J. Life Sci. 13: 492–497.

    Google Scholar 

  14. Cha, W. S., M. Y. Lee, B. S. Cho, S. Y. Park, and D. G. Oh (2004) A study on the mycelial growth of Agrocybe aegerita in flask culture. Kor. J. Life Sci. 14: 560–566.

    Google Scholar 

  15. Cha, W. S., D. B. Lee, S. H. Kang, and D. G. Oh (2003) A study on the cultural characteristics of Pholiota nameko mycelium. Kor. J. Life Sci. 13: 498–504.

    Google Scholar 

  16. Choi, D. B., J. M. Maeng, G. J. Jeung, and W. S. Cha (2007) Exopolysaccharide production and mycelial growth in an air lift bioreactor using Fomitopsis pinicola. J. Microbiol. Biotechnol. 17: 1369–1378.

    CAS  Google Scholar 

  17. Cha, W. S., B. S. Cho, and S. Y. Park (2004) A study on the composition of Cordyceps militaris extract and mycelium. Kor. J. Life Sci. 14: 727–731.

    Google Scholar 

  18. Cha, W. S., M. Y. Lee, B. S. Cho, and S. Y. Park (2004) A study on the composition of seasoning using Lentinus edodes. Kor. J. Life Sci. 14: 829–833.

    Google Scholar 

  19. Choi, D. B., W. S. Cha, S. H. Kang, and B. R. Lee (2004) Effect of Pleurotus ferulae extracts on viability of human lung cancer and cervical cancer cell lines. Biotechnol. Bioprocess Eng. 9: 356–361.

    Article  CAS  Google Scholar 

  20. Choi, D. B., S. S. Park, J. L. Ding, and W. S. Cha (2007) Effects of Fomitopsis pinicola extracts on antioxidant and antitumor activities. Biotechnol. Bioprocess Eng. 12: 516–521.

    Article  CAS  Google Scholar 

  21. Wu, S. H., L. Ryvarden, and T. T. Chang (1997) Antrodia camphorata, new combination of a medicinal mushroom in Taiwan. Bot. Bull. Acad. Sin. 38: 273–275.

    Google Scholar 

  22. Song, T. Y., S. L. Hsu, and G. C. Yen (2005) Induction of apoptosis in human hepatoma cells by mycelia of Antrodia camphorata in submerged culture. J. Ethnopharmacol. 22: 158–167.

    Article  Google Scholar 

  23. Lee, I. H., R. L. Huang, C. T. Chen, H. C. Chen, W. C. Hsu, and M. K. Lu (2002) Antrodia camporata polysaccharides exhibit anti-hepatitus B virus effects. FEMS Microbiol. Lett. 19: 209–214.

    Google Scholar 

  24. Lee, Y. S., E. J. Bak, M. Y. Kim, W. S. Park, J. T. Seo, and Y. J. Yoo (2008) Induction of IL-8 in periodontal ligament Cells by H2O2. J. Microbiol. 46: 579–584.

    Article  CAS  Google Scholar 

  25. Lim, H. S., K. O. Park, N. Nishizawa, S. O. Bae, and M. R. Choi (2008) Cytoxicity pf extracts of from dolsan leaf mustard kimchi treated with lactic acid bacteria on lung and gastric cancer cells. Biotechnol. Bioprocess Eng. 13: 174–181.

    Article  CAS  Google Scholar 

  26. Kang, K. A., S. W. Chae, K. H. Lee, R. Zhang, H. J. You, J. S. Kim, and J. W. Hyun (2005) Antioxidant effect of homogentisic acid on hydrogen peroxide induced oxidative stress in human lung fibroblast cells. Biotechnol. Bioprocess Eng. 10: 556–563.

    Article  CAS  Google Scholar 

  27. Lee, H. J. and Y. W. Seo (2006) Antioxidant properties of erigeron annuus extract and its three phenolic constituents. Biotechnol. Bioprocess Eng. 11: 13–18.

    Article  CAS  Google Scholar 

  28. Choi, D. B., K. A. Cho, M. S. Na, H. S, Choi, Y. O. Kim, D. H. Lim, S. J. Cho, and H. Cho (2008) Effect of Bamboo oil on antioxidative activity and nitrite scavenging activity. J. Ind. Eng. Chem. 14: 765–770.

    Article  CAS  Google Scholar 

  29. Choi, H. S., D. B. Choi, M. S. Na, K. A. Cho, and M. Y. Lee (2009) Antioxidant properties of Achyranthis Radix extract in rats. J. Ind. Eng. Chem. 15: In press.

  30. Meister, A. (1989) Metabolism and function of glutathione. pp. 367–474. In: D. Dolphin, R. Poulson, and R. Auramove (eds.). Glutathione: Chemical, Biochemical, and Medical aspects. John wiley and Sons, NY, USA.

    Google Scholar 

  31. Raes, M., C. Michiels, and J. Relale (1987) Comparative study of the enzymatic defense systems against oxygenderived free radicals: the key role of glutathione peroxidase. Free radical Biol. Med. 3: 3–7.

    Article  CAS  Google Scholar 

  32. Chang, W. S. and J. S. So (1999) Characterization of superoxide dismutase in lactococus lactis. J. Microbiol. Biotechnol. 9: 732–736.

    CAS  Google Scholar 

  33. Song, C. H., E. K. Park, H. J. Suh, Y. S. Lee, J. W. Choi, and K. S. Ra (1999) Purification and characterization of manganese superoxide dismutase from staphylococcus sciuri. J. Microbiol. Biotechnol. 9: 271–275.

    CAS  Google Scholar 

  34. Perchellet, J. and E. M. perchellet (1989) Antioxidants and mutstage carcinogenesis in mouse skin. Free radical Biol. Med. 7: 377–408.

    Article  CAS  Google Scholar 

  35. Sun Y. (1990) Free radicals, antioxidant enzymes, and carcinogensis. Free radical Biol. Med. 8: 583–599.

    Article  CAS  Google Scholar 

  36. Fridovich, I (1995) Superoxide radicals and superoxide dismutases. Annu. Rev. Biochem. 64: 97–112.

    Article  CAS  Google Scholar 

  37. Kim, H. G., G. H. Han, C. Y. Eom, and S. W. Kim (2008) Isolation and taxonomic characterization of a novel type I methanotrophic bacterium. J. Microbiol. 46: 45–50.

    Article  CAS  Google Scholar 

  38. Beckman, J. S. and W. H. Koppenol (1996) Nitroxide, superoxide, and peroxynitrite: the good, the bad, and ugly. Am. J. Physiol. 217: 1421–1437.

    Google Scholar 

  39. Woo, K. S., I. G. Hwang, T. M. Kim, D. J. Kim, J. T. Hong, and H. S. Jeong (2007) Changes in the antioxidant activity of Allium cepa extracts with heat treatment. Food Sci. Biotechnol. 16: 828–831.

    CAS  Google Scholar 

  40. Kang, K. S., N. Yamabea, H. Y. Kim, T. Y. Okamoto, and T. Yokozawa (2007) Increase in the free radical scavenging activities of american ginseng by heat processing and its safety evaluation. J. Ethnopharmacol. 113: 225–232.

    Article  CAS  Google Scholar 

  41. Kang, S. H. (2004) Optimization of Pleurotus ferulae culture conditions for exopolysaccharides production and its biological activity. Ph.D. Thesis. University of Chosun, Gwangju, Korea.

    Google Scholar 

  42. Pitti, R. U., S. A. Marsters, S. Ruppert, C. I. Donahue, A. Moore, and A. Ashkenazi (1996) Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokin family. J. Biol. Chem. 271: 1267–1290.

    Google Scholar 

  43. Guchelaar, H. J., A. Vermes, I. Vermes, and C. Haaden (1997) Apoptosis: molecular mechanism and implication for cancer chemotherapy. Pharm. World Sci. 19: 119–125.

    Article  CAS  Google Scholar 

  44. Park, S. J. (2005) Isolation of lactic acid bacteria producing exopolysaccharide from kefir grain and the functionality of exopolysaccharide. M.S. Thesis. University of Yonsei, Seoul, Korea.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemical Engineering, Chosun University, Gwangju, 501-759, Korea

    Wol-Suk Cha & Ji-Lu Ding

  2. Department of Environmental Health, Cho-dang University, Chonnam, 534-800, Korea

    DuBok Choi

Authors
  1. Wol-Suk Cha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ji-Lu Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. DuBok Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to DuBok Choi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cha, WS., Ding, JL. & Choi, D. Comparative evaluation of antioxidant, nitrite scavenging, and antitumor effects of Antrodia camphorata extract. Biotechnol Bioproc E 14, 232–237 (2009). https://doi.org/10.1007/s12257-008-0291-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-008-0291-1

Keywords

Search

Navigation