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Thymopoietin Beta and Gamma Isoforms as a Potential Diagnostic Molecular Marker for Breast Cancer: Preliminary Data

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Pathology & Oncology Research

Abstract

Thymopoietin (TMPO) is an inner nuclear membrane protein, the coding gene named equally, can give arise to six isoforms by alternative splicing. This gene has been found up regulated in several types of cancer. At present work, we evaluated the TMPO isoforms generated by alternative splicing as well as the protein signal detection in breast cancer samples. TMPO expression was analyzed by immunohistochemistry in tissue microarray containing 46 breast tissue samples including normal (n = 6), benign lesions (n = 18) (fibroadenomas (n = 6), fibrocystic changes (n = 6), ductal hyperplasias (n = 6)) and breast carcinoma (n = 22). Isoforms -α, −β and -γ of TMPO were evaluated using RT-PCR; clinical-pathological correlation analysis were done by mean of X2. Neither the normal nor the benign lesions of the breast showed positive TMPO immunodetection, whilst 45 % of the breast carcinomas were immunopositive (p = 0.000), nine of ten positives carcinomas correspond to the Luminal A subtype. Further, alpha isoform was present in all breast samples analyzed; however, beta and gamma isoforms were only present in ten (p = 0.003) and 17 (p = 0.000), respectively, in the breast cancer samples. According with the present data, we suggest that TMPOβ and -γ isoforms could provide a potential reliable diagnostic marker for breast cancer.

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Acknowledgments

This work was partially supported by a grant FIS-IMSS (Mexico). During this work DMR, KTP, PRM, MMR, LPG and MRE were recipients of a CONACyT fellowship.

Competing Interests

The authors declare that there is not conflict of interest.

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Authors and Affiliations

  1. Laboratorio de Oncología genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI. IMSS, Av. Cuauhtémoc 330, Col. Doctores, México, DF, 06720, Mexico

    Daniel Marrero-Rodríguez, Keiko Taniguchi-Ponciano, Julio Lopez-Sleman, Pablo Romero-Morelos, Monica Mendoza-Rodríguez, Victor Huerta-Padilla, Patricia Piña, Miriam Rodriguez-Esquivel, Ricardo Lopez-Romero, Jorge Parrazal-Romero & Mauricio Salcedo

  2. Laboratorio de Biotecnología, Universidad Autonóma de Ciudad Juárez, Chihuahua, Mexico

    Daniel Marrero-Rodríguez & Florinda Jimenez-Vega

  3. Servicio de Ginecología Oncológica, Hospital de Oncología CMN-SXXI, IMSS, Mexico, Mexico

    Israel Garcia

  4. Servicio de Patología, Hospital de Oncología CMN-SXXI, IMSS, Mexico, Mexico

    Alejandra Mantilla

  5. Clínica de Mama, Hospital General de Zona #35, IMSS, Cd. Juárez, Chihuahua, Mexico

    Armando Duarte

  6. Departamento de Patología, Hopital General, Cd. Juarez, Chihuahua, Mexico

    Salvador Tobias-Alonso

  7. Unidad de Quimioterapia, Servicio de Oncología, Hospital General de Mexico, S.S., Mexico, Mexico

    Mario Alvarez-Blanco

Authors
  1. Daniel Marrero-Rodríguez
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  2. Keiko Taniguchi-Ponciano
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  3. Julio Lopez-Sleman
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  4. Pablo Romero-Morelos
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  5. Monica Mendoza-Rodríguez
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  6. Israel Garcia
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  7. Victor Huerta-Padilla
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  8. Alejandra Mantilla
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  9. Armando Duarte
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  10. Patricia Piña
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  11. Miriam Rodriguez-Esquivel
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  12. Ricardo Lopez-Romero
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  14. Salvador Tobias-Alonso
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  15. Florinda Jimenez-Vega
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  16. Mario Alvarez-Blanco
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  17. Mauricio Salcedo
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Corresponding author

Correspondence to Mauricio Salcedo.

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Marrero-Rodríguez, D., Taniguchi-Ponciano, K., Lopez-Sleman, J. et al. Thymopoietin Beta and Gamma Isoforms as a Potential Diagnostic Molecular Marker for Breast Cancer: Preliminary Data. Pathol. Oncol. Res. 21, 1045–1050 (2015). https://doi.org/10.1007/s12253-015-9907-x

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  • DOI: https://doi.org/10.1007/s12253-015-9907-x

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