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Possible Prognostic Value of BORIS Transcript Variants Ratio in Laryngeal Squamous Cell Carcinomas – a Pilot Study

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Pathology & Oncology Research

Abstract

BORIS is a paralog of a highly conserved, multi-functional chromatin factor CTCF. Unlike CTCF, which has been shown to possess tumor-suppressive properties, BORIS belongs to the “cancer/testis antigen” family normally expressed only in germ cells and aberrantly activated in a variety of tumors. The consequences of BORIS expression, relative abundance of its isoforms, and its role in carcinogenesis have not been completely elucidated. It activates transcription of hTERT and MYC, genes relevant for laryngeal carcinoma progression. In this study, BORIS expression has been analyzed at the transcriptional level by RT-PCR and protein level by semi-quantitative immunohistochemistry in 32 laryngeal squamous cell carcinomas and adjacent non-tumorous tissue. BORIS was detected in 44 % (14/32) laryngeal squamous cell carcinoma samples, while it was detected only in one normal, tumor-adjacent tissue sample. Tree based survival analysis, using the recursive partitioning algorithm mvpart, extracted the ratio of relative abundance of BORIS transcript variants containing exon 7 (BORIS 7+) and those lacking exon 7 (BORIS 7−) as an independent prognostic factor associated with disease relapse during a 5-year follow-up period. Patients having BORIS 7+/BORIS 7− ratio ≥1 had a higher rate of disease relapse than patients with BORIS 7+/BORIS 7− ratio <1. Hazard ratio for that group, based on Cox Proportional Hazard Regression, was 3.53. This is the first study analyzing expression of BORIS protein and transcript variants in laryngeal squamous cell carcinoma relative to its possible prognostic value for recurrence and overall survival.

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Acknowledgments

This work was funded by grants 098-0982464-2511 and 108-1081870-1884 from the Ministry of Science, Education and Sport, Republic of Croatia.

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Authors and Affiliations

  1. Laboratory for Epigenomics, Ruđer Bošković Institute, Bijenička cesta 54, 10002, Zagreb, Croatia

    Renata Novak Kujundžić, Ivana Grbeša & Koraljka Gall Trošelj

  2. Department of Otorhinolaryngology and Head and Neck Surgery, Sestre Milosrdnice University Hospital, 10000, Zagreb, Croatia

    Mirko Ivkić

  3. Department of Pathology «Ljudevit Jurak», Sestre Milosrdnice University Hospital, 10000, Zagreb, Croatia

    Božo Krušlin

  4. Center for NMR, Ruđer Bošković Institute, Bijenička cesta 54, 10002, Zagreb, Croatia

    Paško Konjevoda

  5. Bijenička 54, 10000, Zagreb, Croatia

    Renata Novak Kujundžić

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  1. Renata Novak Kujundžić
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Correspondence to Renata Novak Kujundžić.

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Novak Kujundžić, R., Grbeša, I., Ivkić, M. et al. Possible Prognostic Value of BORIS Transcript Variants Ratio in Laryngeal Squamous Cell Carcinomas – a Pilot Study. Pathol. Oncol. Res. 20, 687–695 (2014). https://doi.org/10.1007/s12253-014-9749-y

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