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MicroRNA Expression Profiles in Kaposi’s Sarcoma

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Pathology & Oncology Research

Abstract

Kaposi’s sarcoma (KS) is a mesenchymal tumor, caused by Human herpesvirus 8 (HHV8) with molecular and cytogenetic changes poorly understood. To gain further insight on the underlying molecular changes in KS, we performed microRNA (miRNA) microarray analysis of 17 Kaposi’s sarcoma specimens. Three normal skin specimens were used as controls. The most significant differentially expressed miRNA were confirmed by quantitative reverse transcriptase polymerase chain reaction (RT-PCR). We detected in KS versus normal skin 185 differentially expressed miRNAs, 76 were upregulated and 109 were downregulated. The most significantly downregulated miRNAs were miR-99a, miR-200 family, miR-199b-5p, miR-100 and miR-335, whereas kshv-miR-K12-4-3p, kshv-miR-K12-1, kshv-miR-K12-2, kshv-miR-K12-4-5p and kshv-miR-K12-8 were significantly upregulated. High expression levels of kshv-miR-K12-1 (p = 0.004) and kshv-miR-K12-4-3p (p = 0.001) was confirmed by RT-PCR. The predicted target genes for differentially expressed miRNAs included genes which are involved in a variety of cellular processes such as angiogenesis (i.e. THBS1) and apoptosis (i.e. CASP3, MCL1), suggesting a role for these miRNAs in Kaposi’s sarcoma pathogenesis.

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Acknowledgments

We thank Neda Mosakhani, M.D. for the guidance and help in performing in PCR. This project was supported by prof. Sakari Knuutila’s grant from the special state subsidy research funds appropriated to the Helsinki and Uusimaa Hospital District (HUS EVO) and the TEKES (the Finnish Funding Agency for Technology and Innovation) Multibio project (grant no. 40141/07), Finland, as well as by the TD Project Code 222 (CNCSIS), Romania.

Disclosure/Conflict of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 050095, Bucharest, Romania

    Ana Maria Catrina (Ene), Elena Ionica & Aura Chitu

  2. Department of Pathology, Haartman Institute and HUSLAB, University of Helsinki, PO Box 21, Haartmaninkatu 3, 00014, Helsinki, Finland

    Ioana Borze, Mohamed Guled, Gayle Leen & Sakari Knuutila

  3. Department of Pathology, “Carol Davila” University of Medicine and Pharmacy, 050471, Bucharest, Romania

    Mariana Costache & Maria Sajin

  4. Department of Information and Computer Science, Aalto University School of Science, 02150, Espoo, Finland

    Gayle Leen

Authors
  1. Ana Maria Catrina (Ene)
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  2. Ioana Borze
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  3. Mohamed Guled
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  4. Mariana Costache
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  7. Elena Ionica
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  9. Sakari Knuutila
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Correspondence to Sakari Knuutila.

Additional information

Ana Maria Catrina (Ene) and Ioana Borze equally contributed to this study.

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Catrina (Ene), A.M., Borze, I., Guled, M. et al. MicroRNA Expression Profiles in Kaposi’s Sarcoma. Pathol. Oncol. Res. 20, 153–159 (2014). https://doi.org/10.1007/s12253-013-9678-1

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  • DOI: https://doi.org/10.1007/s12253-013-9678-1

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