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Expression Levels of MicroRNA-192 and -215 in Gastric Carcinoma

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Pathology & Oncology Research

Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. Accumulating studies have shown aberrant miRNA expression plays an important role in many tumor types. miR-192 and -215, which have the same “seed region”, have not been comprehensively investigated using a large number of cases in gastric cancer. The total RNA was extracted from 118 gastric cancer tissues and three gastric cancer cell lines as well as matched non-tumor adjacent tissues (NATs). After polyadenylation and reverse transcription, expression levels of miR-192 and -215 were determined by real-time PCR and calculation using the 2-∆∆CT method for evaluation of the association between miR-192, and -215 expression levels and clinicopathological characteristics. There were no significant differences in miR-192 and -215 expression levels between gastric cancer tissues and non-tumor counterparts (both p > 0.05, paired t-test). Interestingly, miR-192 and -215 were down-regulated in MGC-803 cells, BGC-823 cells and SGC-7901 cells (all p < 0.01, paired t-test). Also, the down-regulation of miR-192 and -215 was demonstrated to be associated with increased tumor sizes (both p = 0.003, Mann–Whitney U test) and advanced Borrmann type tumors (p = 0.015 and p = 0.044, respectively, Kruskal-Wallis H test). Moreover, the expression of miR-192 was significantly lower in the pT4 stage of gastric cancer than in pT1, pT2 and pT3 stages (p = 0.026). Furthermore, there was a strong correlation between miR-192 and -215 in gastric cancer tissues (p < 0.001, Pearson regressions). miR-192 and -215 might be related to the proliferation and invasion of gastric cancer. Potentially, they could become important biomarkers.

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Acknowledgement

This work was supported by National Science Foundation of China (No. 30972879 and No. 81172370), Specialized Research Fund for the Doctoral Program of Higher Education (No. 200801590006) and Natural Science Foundation of Liaoning Province (No. 20092129).

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Authors and Affiliations

  1. Department of Surgical Oncology and General Surgery, First Hospital of China Medical University, Shenyang, 110001, People’s Republic of China

    Yeunpo Chiang, Zhenning Wang, Yongxi Song, Zhuangkai Liu, Jinliang Zhu & Huimian Xu

  2. Department of Gynecology and Obstetrics, ShengJing Hospital of China Medical University, Shenyang, People’s Republic of China

    Xin Zhou & Fang Zhao

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  1. Yeunpo Chiang
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  2. Xin Zhou
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  3. Zhenning Wang
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  4. Yongxi Song
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  5. Zhuangkai Liu
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  6. Fang Zhao
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  7. Jinliang Zhu
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Corresponding author

Correspondence to Zhenning Wang.

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Yeunpo Chiang and Xin Zhou contributed equally to this work

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Chiang, Y., Zhou, X., Wang, Z. et al. Expression Levels of MicroRNA-192 and -215 in Gastric Carcinoma. Pathol. Oncol. Res. 18, 585–591 (2012). https://doi.org/10.1007/s12253-011-9480-x

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  • DOI: https://doi.org/10.1007/s12253-011-9480-x

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