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Allelic Loss of 10q23.3, the PTEN Gene Locus in Cervical Carcinoma from Northern Indian Population

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Pathology & Oncology Research

Abstract

Cervical cancer is one of the most common malignant diseases affecting women worldwide. Studies on loss of heterozygosity have been made for PTEN gene specific microsatellite markers in malignancies like breast, ovary and lungs and the results have shown a significant association. However the role of this gene is not clearly understood in cervical cancer from Indian population. A total of 135 cervical carcinoma tissues samples were analyzed for loss of heterozygosity. DNA was isolated from the samples and their matched control specimens. Polymerase chain reaction was performed using primer specific for two intragenic markers (D10S198 & D10S192) and one marker (D10S541) in flanking region and further electrophoresed on 8% denaturing polyacrylamide gel. Overall, 31 out of 133(23%) informative cases showed loss of heterozygosity in at least one locus in the region examined. The percentage of loss of heterozygosity for these markers ranged from 8% (D10S192) to 13% (D10S198). Loss of heterozygosity was more frequently detected in intragenic region (D10S198 & D10S192) than in flanking region, D10S541 (21% versus 9%). These data argue that PTEN is a tumor suppressor gene whose inactivation may play an important role in the carcinoma of uterine cervix.

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Acknowledgement

The financial assistance provided by the University Grants Commision (UGC), New Delhi, is gratefully acknowledged. The assistance of M. Irshad is acknowledged with thanks.

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Authors and Affiliations

  1. Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, Maulana Mohammad Ali Jauhar Marg, New Delhi-, 110025, India

    M. Moshahid Alam Rizvi, M. Shabbir Alam, Syed Jafar Mehdi & Asgar Ali

  2. Department of Medical Lab Technology, Faculty of Applied Medical Sciences, Jazan University, Jizan, Kingdom of Saudi Arabia

    M. Shabbir Alam

  3. Department of Obstetrics & Gynecology, LNJP/MAMC Campus, New Delhi-, 110002, India

    Swaraj Batra

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  1. M. Moshahid Alam Rizvi
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Correspondence to M. Moshahid Alam Rizvi.

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Rizvi, M.M.A., Alam, M.S., Mehdi, S.J. et al. Allelic Loss of 10q23.3, the PTEN Gene Locus in Cervical Carcinoma from Northern Indian Population. Pathol. Oncol. Res. 18, 309–313 (2012). https://doi.org/10.1007/s12253-011-9446-z

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