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Increased Expression of Prohibitin and its Relationship with Poor Prognosis in Esophageal Squamous Cell Carcinoma

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Pathology & Oncology Research

Abstract

Prohibitin, a potential tumor suppressor, has been shown to be an anti- proliferative protein, a regulator of cell-cycle progression and in apoptosis. Recently, it was found to be over-expressed in breast cancer and gastric cancer, and it has been suggested as a biomarker in those diseases. To clarify the role and the prognostic significance of prohibitin expression in esophageal squamous cell carcinoma (ESCC), we analyzed the expression in ESCC and their corresponding nonneoplastic epithelia tissues by immunohistochemistry(IHC), Western blotting and real-time quantitative reverse transcription polymerase chain reaction(QRT-PCR).The relationship between prohibitin expression and clinicopathological variables was examined by statistical analysis. The findings suggested the up-regulation of prohibitin play an important role in the carcinogenesis of ESCC. The over-expression of prihibitin was significantly correlated with the depth of tumor, lymph node metastasis, distant metastasis, lymphatic invasion and vascular invasion of ESCC. These results suggested that prohibitin(+), lymph node metastasis and distant metastasis could be the independent risk factors for worse prognosis in ESCC patients.

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Abbreviations

EC:

esophageal cancer

ESCC:

esophageal squamous cell carcinoma

IHC:

immunohistochemistry

QRT-PCR:

real-time quantitative reverse-transcription polymerase chain reaction

TNM:

tumor, node, metastases

AJCC:

American Joint Committee on Cancer

TMA:

tissue microarray

PBS:

phosphate buffer solution

DAB:

diaminobenzidine

PMSF:

phenylmethylsulphonyl fluoride

PVDF:

polyvinylidene difluoride

HRP:

horseradish peroxidase

ECL:

enhanced chemiluminescence

RB:

retinoblastoma tumor suppressor protein

BPH:

Benign Prostatic Hyperplasia

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Acknowledgements

This work was supported partly by a grant from the Innovation Project of Central South University (No.2340-76208). We would like to thank Dr. Zhen Zhang for his technical assistance.

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Authors and Affiliations

  1. Department of Pathology, Xiangya Basic Medical College, Central South University, Changsha, China

    Hong-Zheng Ren, Peng Wang, Guo-qing Pan, Ji-Fang Wen & Hua Fu

  2. Department of Pathology, Changzhi Medical College, Changzhi, China

    Jin-Sheng Wang

  3. Department of Health Statistics and Epidemiology, Public Health College, Central South University, Changsha, China

    Xu-zheng Shan

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  1. Hong-Zheng Ren
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  2. Jin-Sheng Wang
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Correspondence to Ji-Fang Wen.

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Ren, HZ., Wang, JS., Wang, P. et al. Increased Expression of Prohibitin and its Relationship with Poor Prognosis in Esophageal Squamous Cell Carcinoma. Pathol. Oncol. Res. 16, 515–522 (2010). https://doi.org/10.1007/s12253-009-9242-1

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  • DOI: https://doi.org/10.1007/s12253-009-9242-1

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