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Pheno- and Genotypic Features of Epstein-Barr Virus Associated B-Cell Lymphoproliferations in Peripheral T-Cell Lymphomas

  • Published:
Pathology & Oncology Research

Abstract

Among the 300 peripheral T-cell lymphomas (PTCL) searched for EBV positive non-resting B-cells by EBER in situ hybridization 12 have been identified with various forms of EBV-driven B-cell proliferation. This could be categorized into three major forms. i. In the first form scattered immature, mononuclear B-cells of immuno-, centroblastic type with CD20+. CD30+ CD45+, LMP1+ phenotype, reactive appearance and polyclonal immunoglobulin heavy chains gene rearrangement (IgH-R) were admixed to the PTCL cells. ii. The second form mimicked diffuse large B-cell lymphoma as homogenous sheets, largely demarcated from the PTCL, of mononuclear, immature B-cell of CD20+, CD30+, CD45+, LMP1+, EBNA-2+ phenotype but with lack of monoclonal IgH-R were present. iii. In the third form scattered Hodgkin-Reed-Sternberg (HRS) type of cells were noticed which exhibited the CD15+/−, CD20−/+, CD30+, CD45−, LMP1+, EBNA-2- phenotype and in 50% showed clonal IgH gene rearrangement in whole tissue DNA extract. The IgH associated transcription factors’ (OCT2, BOB.1/OBF.1, PU.1) expression patterns in these cells corresponded to those of HRS cells in cHL. Based on analysis of 65 PTCLs, we have identified in the positive cases a highly significant increase of EBV+ small, reactive, resting B-cell compartment (75.9 / 100 HPF in PTCL vs. 1.5 / 100 HPF in control lymph nodes) likely to be due to the decreased immune surveillance. This progressive accumulation of EBV+ by-stander B-cell population in PTCLs might be the source of various B-cell proliferations, which in any form represent major diagnostic pitfalls and require a careful differential diagnostic procedure.

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Abbreviations

CDR:

complementary determining region

CHL:

classical Hodgkin lymphoma

DLBCL:

diffuse large B-cell lymphoma

EBER:

Epstein Barr early response gene

EBNA:

Epstein Barr nuclear antigen

EBV:

Epstein Barr virus

FR:

framework region

HPF:

high power field

HRS:

Hodgkin Reed Sternberg

IgH-R:

immunoglobulin heavy chain gene rearrangement

LBC:

large B-cell

LBCR-TCL:

large B-cell rich T-cell lymphoma

LMP-1:

latent membrane protein-1

NOS:

not otherwise specified

PCR:

polymerase chain reaction

PTCL:

peripheral T-cell lymphoma

PTCL-LB:

PTCL with proliferation of large B-cells

TCR-BCL:

T-cell rich B-cell lymphoma

TCR-γ-R:

T-cell receptor gamma gene rearrangement

TF:

transcription factor

TL:

T-cell lymphoma

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Acknowledgement

This work was supported by the grant 268/2006 sponsored by the Hungarian Scientific Council of Health (ETT).

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Authors and Affiliations

  1. Department of Pathology, University of Pécs Medical Center, 12 Szigeti Str., 7624, Pécs, Hungary

    Gábor Smuk, László Kereskai, Balázs Márton, Zsófia Nagy, Ágnes Lacza & László Pajor

  2. 3rd Department of Medicine, Medical and Health Center, University of Debrecen, Debrecen, Hungary

    Árpád Illés & Katalin Keresztes

Authors
  1. Gábor Smuk
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  2. Árpád Illés
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  3. Katalin Keresztes
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  4. László Kereskai
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  5. Balázs Márton
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  6. Zsófia Nagy
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  7. Ágnes Lacza
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  8. László Pajor
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Correspondence to László Pajor.

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Smuk, G., Illés, Á., Keresztes, K. et al. Pheno- and Genotypic Features of Epstein-Barr Virus Associated B-Cell Lymphoproliferations in Peripheral T-Cell Lymphomas. Pathol. Oncol. Res. 16, 377–383 (2010). https://doi.org/10.1007/s12253-009-9233-2

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  • DOI: https://doi.org/10.1007/s12253-009-9233-2

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