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Identification of Rho GTPase Activating Protein 6 Isoform 1 Variant as a New Molecular Marker in Human Colorectal Tumors

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Pathology & Oncology Research

Abstract

The early diagnosis of colorectal cancer (CRC) is important because it is one of the most readily curable of all cancers, if detected early. However, the sensitivity of current markers is low. Immunostaining intensity for the monoclonal antibody Hb3 in CRC cell lines and tissues was stronger than in controls. Interestingly, this was associated with a low level of tumor differentiation. We used Hb3-coupled affinity chromatography to search for a corresponding Hb3 antigen as a candidate biomarker for early detection, and identified a Rho GTPase activating protein 6 (RhoGAP6) isoform 1 variant as an Hb3 antigen by mass spectrometry. Using reverse transcription polymerase chain reaction and western blot analysis, we confirmed that the expression levels of this variant were elevated in aberrant cells and tissues. Thus, the RhoGAP6 isoform 1 variant might serve as a biomarker for the development and progression of CRC.

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Abbreviations

CRC:

Colorectal cancer

RhoGAP6:

Rho GTPase activating protein 6

mAb:

monoclonal antibody

MALDI-TOF MS:

Matrix assisted laser desorption ionization time-of-flight mass spectrometry

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Authors and Affiliations

  1. Cancer Research Institute, Xiangya Medical School, Central South University, 110 Xiangya Road, Changsha, 410078, Hunan Province, China

    Fengjie Guo, Yan Liu, Jian Huang, Yuehui Li, Guohua Zhou, Yalin Li, Jiajia Wang, Pingli Xie & Guancheng Li

  2. Department of Pathology, The First Hospital of Changsha, Changsha, Hunan Province, China

    Di Wang

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  1. Fengjie Guo
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  2. Yan Liu
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  3. Jian Huang
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  4. Yuehui Li
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  5. Guohua Zhou
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  6. Di Wang
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  7. Yalin Li
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  8. Jiajia Wang
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  9. Pingli Xie
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  10. Guancheng Li
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Correspondence to Guancheng Li.

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Guo, F., Liu, Y., Huang, J. et al. Identification of Rho GTPase Activating Protein 6 Isoform 1 Variant as a New Molecular Marker in Human Colorectal Tumors. Pathol. Oncol. Res. 16, 319–326 (2010). https://doi.org/10.1007/s12253-009-9226-1

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  • DOI: https://doi.org/10.1007/s12253-009-9226-1

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