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Serum Levels of Angiogenic Factors and their Prognostic Relevance in Bladder Cancer

  • Original Paper
  • Published:
Pathology & Oncology Research

Abstract

Angiogenesis plays a critical role in tumor growth. VEGF, angiopoietins (Ang-1, Ang-2) and their tyrosine kinase receptor Tie2 are major regulators of angiogenesis. The aim of this study was to evaluate the prognostic value of the serum levels of these factors in bladder cancer. We analyzed the serum samples of 117 bladder cancer patients and 64 healthy volunteers by enzyme linked immunosorbent assay (ELISA) for Ang-1, Ang-2, VEGF and the extracellular domain of Tie2. The statistical evaluation of the obtained data was performed via Kaplan–Meier log-rank test, univariate Cox analyses as well as Cox proportional hazards regression model. Serum Ang-1 levels of bladder cancer patients were significantly higher (p < 0.001), while soluble Ang-2 and Tie2 levels were significantly lower (p = 0.016 and p = 0.001 respectively) in patients than those in controls. Cox univariate analysis revealed high sTie2 serum level as a risk factor for metastasis and as a borderline significant risk factor for disease related death (p = 0.022 and p = 0.081 respectively). These correlations were independent from tumor stage and grade in a Cox multivariate model (p = 0.016 and p = 0.069). These data indicate that the serum levels of analyzed angiogenic factors do change characteristically in bladder cancer. The soluble extracellular serum level of Tie2 may provide a stage and grade independent diagnostic tool to select a high risk group of bladder cancer patients.

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Abbreviations

HR:

hazard ratio

CI:

confidence interval

TEM:

Tie2-expressing monocyte

TCC:

transitional cell carcinoma

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Acknowledgement

We thank Jacqueline Wittschier for excellent technical assistance. Grant support from the German National Federal Ministry of Education and Research (0313659B) is also acknowledged.

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Authors and Affiliations

  1. Experimental Urology, Department of Urology, University of Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Germany

    Tibor Szarvas, Tobias Jäger, Falk Droste, Markus Becker & Herbert Rübben

  2. 1st Institute of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

    Ilona Kovalszky

  3. Department of Urology, Semmelweis University, Budapest, Hungary

    Imre Romics

  4. Institute of Anatomy, University Hospital of Essen, Essen, Germany

    Süleyman Ergün

Authors
  1. Tibor Szarvas
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  2. Tobias Jäger
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Correspondence to Tibor Szarvas.

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Szarvas, T., Jäger, T., Droste, F. et al. Serum Levels of Angiogenic Factors and their Prognostic Relevance in Bladder Cancer. Pathol. Oncol. Res. 15, 193–201 (2009). https://doi.org/10.1007/s12253-008-9107-z

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  • DOI: https://doi.org/10.1007/s12253-008-9107-z

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