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Ryanodine Receptor Expression Correlates with Tumor Grade in Breast Cancer

  • Original Paper
  • Published:
Pathology & Oncology Research

Abstract

Ryanodine receptors (RyRs) have been previously implicated in the proliferation of human T-lymphocytes and melanocytes as well as in the motility of astrocytes. We have examined RyR expression in 57 ductal, human breast cancer specimens, by immunohistochemistry. Moderate to high RyR immunostaining was found in 47 (82%) of the specimens. There was a direct correlation between RyR levels and tumor grade (r = 0.48, p = 0.0002). We have also examined the effect of the RyR agonist 4-chloro-m-cresol on the in-vitro growth of two human breast cancer cell lines, MCF-7 and MDA-MB-231. Treatment with 4-chloro-m-cresol inhibited the growth of both breast cancer cell lines, in a dose-dependent manner, with half-maximal inhibition observed at 30 to 50 μg/mL (210–351 μM). Our data suggest that RyR could serve as prognostic indicator and/or as a target for breast cancer treatment.

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Abbreviations

Ca2+:

calcium ion

RyR:

ryanodine receptor

Bca:

breast cancer

PBS:

phosphate buffered saline

RT:

room temperature

HRP:

horseradish peroxidase

DAB:

3,3′-diaminobenzidine

L:

Leibovitz’s

ER+ve:

estrogen receptor alpha positive

ER−ve:

estrogen receptor alpha negative

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Acknowledgements

This work was supported, in part, by South Carolina (SC) INBRE (IDeA Network of Biomedical Research Excellence) Grant Number RR16461 and the SC LIFE Undergraduate Research Program.

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Authors and Affiliations

  1. Biology Department, Claflin University, 400 Magnolia Street, Orangeburg, SC, 29115, USA

    Mansoor Abdul, Sian Ramlal & Naseema Hoosein

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  1. Mansoor Abdul
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  2. Sian Ramlal
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  3. Naseema Hoosein
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Correspondence to Naseema Hoosein.

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Abdul, M., Ramlal, S. & Hoosein, N. Ryanodine Receptor Expression Correlates with Tumor Grade in Breast Cancer. Pathol. Oncol. Res. 14, 157–160 (2008). https://doi.org/10.1007/s12253-008-9045-9

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  • DOI: https://doi.org/10.1007/s12253-008-9045-9

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