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Different Expression of Occludin and ZO-1 in Primary and Metastatic Liver Tumors

  • Original Paper
  • Published:
Pathology & Oncology Research

Abstract

Tight junction (TJ) components were found to be correlated with carcinogenesis and tumor development. TJs are composed of three main integral membrane proteins; occludin, claudins and JAMs. Alteration of the TJ protein expression may play an important role in the process of cell dissociation, which is among the first steps of tumor invasion and metastasis. Reduced expression of ZO-1 has been reported to be associated with invasion of several tumors. The aim of the present study was to detect differences between occludin and ZO-1 expression in normal liver samples, HCCs and colorectal liver metastases. Expression of occludin and ZO-1 was analysed in 25 surgically removed human hepatocellular carcinomas (HCC) and 25 human colorectal liver metastases. Gene expression levels were measured by real-time RT PCR, protein expression was determined by immunohistochemistry, comparing tumors with the surrounding nontumorous parenchyma and with seven normal liver samples. Occludin and ZO-1 mRNAs showed significant downregulation in HCCs in comparison with normal liver and were also downregulated in the metastases when compared with normal liver. Occludin and ZO-1 proteins were weakly expressed on hepatocytes in normal liver, while strong expression was found on bile canaliculi. In HCCs occludin and ZO-1 did not show immunopositivity on tumor cells, while colorectal metastatic tumors revealed high levels of these molecules. HCCs and metastases are characterized by markedly different protein expression pattern of occludin and ZO-1, which phenomenon might be attributed to the different histogenesis of these tumors.

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Abbreviations

ZO-1:

Zonula Occludens-1

TJ:

Tight Junction

HCC:

Hepatocellular Carcinoma

CRC:

Colorectal Cancer

ECM:

Extracellular Matrix

JAM:

Junctional Adhesion Molecule

TER:

Transepithelial Electric Resistance

MAGUK:

Membrane Associated Guanylate Kinase

PCR:

Polymerase Chain Reaction

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Acknowledgement

This project was supported by grants: National Office for Research and Technology (NKTH) No NKFP-1/0023/2002 and Hungarian Research Foundation (OTKA) No T049559.

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Authors and Affiliations

  1. 2nd Department of Pathology, Semmelweis University, 93 Üllői út, Budapest, 1091, Hungary

    Erika Orbán, Erzsébet Szabó, Gábor Lotz, Csilla Páska, Zsuzsa Schaff & András Kiss

  2. Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, Budapest, Hungary

    Erika Orbán

  3. 1st Department of Surgery, Semmelweis University, Budapest, Hungary

    Péter Kupcsulik

Authors
  1. Erika Orbán
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  2. Erzsébet Szabó
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  3. Gábor Lotz
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  4. Péter Kupcsulik
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  5. Csilla Páska
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  6. Zsuzsa Schaff
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  7. András Kiss
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Correspondence to Zsuzsa Schaff.

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Orbán, E., Szabó, E., Lotz, G. et al. Different Expression of Occludin and ZO-1 in Primary and Metastatic Liver Tumors. Pathol. Oncol. Res. 14, 299–306 (2008). https://doi.org/10.1007/s12253-008-9031-2

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  • DOI: https://doi.org/10.1007/s12253-008-9031-2

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