References
Chen Q, Tang K, Zhang X, Chen P, Guo Y (2018) Establishment of pseudovirus infection mouse models for in vivo pharmacodynamics evaluation of filovirus entry inhibitors. Acta Pharmaceutica Sinica B 8:200–208
Panina AA, Dementieva IG, Aliev TK, Toporova VA, Balabashin DS, Bokov MN, Pozdnyakova LP, Shchemchukova OB, Dolgikh DA, Sveshnikov PG, Kirpichnikov MP (2017) Recombinant Antibodies to the Ebola Virus Glycoprotein. Acta Naturae 9:84–91
Qiu C, Huang Y, Zhang A, Tian D, Wan Y, Zhang X, Zhang W, Zhang Z, Yuan Z, Hu Y (2013) Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus. Emerg Infect Dis 19:1685–1687
Zhao G, Du L, Ma C, Li Y, Li L, Poon VK, Wang L, Yu F, Zheng BJ, Jiang S, Zhou Y (2013a) A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV. Virol J 10:266
Zhao GY, Du LY, Ma CQ, Li Y, Li L, Poon VKM, Wang LL, Yu F, Zheng BJ, Jiang SB, Zhou YS (2013b) A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV. Virol J 10(1):1–8
Zheng X, Wong G, Zhao Y, Wang H, He S, Bi Y, Chen W, Jin H, Gai W, Chu D, Cao Z, Wang C, Fan Q, Chi H, Gao Y, Wang T, Feng N, Yan F, Huang G, Zheng Y, Li N, Li Y, Qian J, Zou Y, Kobinger G, Gao GF, Qiu X, Yang S, Xia X (2016) Treatment with hyperimmune equine immunoglobulin or immunoglobulin fragments completely protects rodents from Ebola virus infection. Sci Rep 6:24179
Acknowledgements
We thank Gary Wong for help with experiments involving authentic EBOV. We thank our lab members and colleagues at Jilin University for helpful discussions during the course of the study. This research was supported by the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant 2016ZX10004222).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Animal and Human Rights Statement
All animal studies in this work were approved by the Animal Care and Use Committee of the Chinese People’s Liberation Army (No. SYXK2009-045). All efforts were made to minimize animal suffering.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Cao, Z., Jin, H., Wong, G. et al. The Application of a Safe Neutralization Assay for Ebola Virus Using Lentivirus-Based Pseudotyped Virus. Virol. Sin. 36, 1648–1651 (2021). https://doi.org/10.1007/s12250-021-00405-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12250-021-00405-8