Abstract
The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor (F0) and then cleaved into a disulfide-linked F1 and F2 subunits during the process of protein maturation and secretion. To facilitate further investigation into the structure and function of F protein during virus infection, monoclonal antibodies (mAbs) against the F2 subunit of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) (HaF) were generated. Two kinds of mAbs were obtained according to their different recognition epitopes: one kind of mAbs, as represented by 38F10, recognizes amino acid (aa) 85 to 123 of F2 and the other kind, represented by 44D11, recognizes aa 148 to 173 of F2. Western blot and immunofluorescence assay confirmed that both of the mAbs recognized the F protein expressed in HearNPV infected cells, however, only 44D11 could neutralize HearNPV infection. The results further showed that 44D11 may not interact with a receptor binding epitope, rather it was demonstrated to inhibit syncytium formation in cells expressing the HaF protein. The results imply that the monoclonal antibody 44D11 recognizes a region within HaF2 that may be involved in the F-mediated membrane fusion process.
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Acknowledgments
This work was supported by the grants from the National Science Foundation of China (No. 31370191 and 31621061), the Strategic Priority Research Program of the Chinese Academy of Sciences (grant XDB11030400), and Open Research Fund Program of the Key Laboratory of Agricultural and Environmental Microbiology, Chinese Academy of Sciences. We acknowledge the Core Facility and Technical Support of Wuhan Institute of Virology for technical assistance. The authors would like to thank Dr. Xiulian Sun for the great help in statistical analysis.
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ORCID: 0000-0003-4697-9501
ORCID: 0000-0001-8701-3530
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Zou, Z., Liu, J., Wang, Z. et al. Characterization of two monoclonal antibodies, 38F10 and 44D11, against the major envelope fusion protein of Helicoverpa armigera nucleopolyhedrovirus. Virol. Sin. 31, 490–499 (2016). https://doi.org/10.1007/s12250-016-3831-4
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DOI: https://doi.org/10.1007/s12250-016-3831-4