Skip to main content
SpringerLink
Log in

Different Genetic Mechanisms Control Foliar and Tuber Resistance to Phytophthora infestans in Wild Potato Solanum verrucosum

  • Published:
American Journal of Potato Research Aims and scope Submit manuscript

Abstract

Late blight, caused by the oomycete pathogen, Phytophthora infestans, is a devastating disease of potatoes and tomatoes. A key long-term management strategy for combating this disease is to develop potato cultivars with broad-spectrum and durable resistance through identification and integration of major resistance genes. In support of previous results, we have determined that some accessions of the wild potato species Solanum verrucosum contain strong foliar resistance to P. infestans while others are susceptible. In addition, whole- and wounded-tuber inoculations revealed that most accessions lacked resistance to tuber infection. However, one accession (PI 570643) contained little foliar resistance but expressed tuber resistance, suggesting that expression of these two phenotypes in this species is not correlated.

Resumen

El tizón tardío, causado por el patógeno oomycete Phytophthora infestans,es una enfermedad devastadora de papas y tomates. Una estrategia clave de manejo a largo plazo para combatir esta enfermedad es el desarrollo de cultivares de papa con un amplio espectro de resistencia durable, mediante la identificación e integración de genes mayores de resistencia. Como respaldo a resultados previos, hemos determinado que algunas introducciones de la especie silvestre de papa Solanum verrucosum contiene fuerte resistencia foliar a P. infestans, mientras que otras son susceptibles. Además, inoculaciones a tubérculos completos y heridos revelaron que la mayoría de las introducciones carecían de resistencia a la infección de tubérculo. No obstante, una introducción (PI 570643) contenía poca resistencia foliar pero expresó resistencia de tubérculo, sugiriendo que la expresión de estos dos fenotipos en esta especie no está correlacionada.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Becktell, M.C., C.D. Smart, C.H. Haney, and W.E. Fry. 2006. Host-pathogen interactions between Phytophthora infestans and the Solanaceous hosts Calibrachoa × hybridus, Petunia × hybrida, and Nicotiana benthamiana. Plant Disease 90: 24–32.

    Article  CAS  Google Scholar 

  • Caten, C.E. and J.L. Jinks. 1968. Spontaneous variability of single isolates of Phytophthora infestans. Canadian Journal of Botany 46: 329–347.

    Article  Google Scholar 

  • Collins, A., D. Milbourne, L. Ramsay, R. Meyer, C. Chatot-Balandras, P. Oberhagemann, W. De Jong, C. Gebhardt, E. Bonnel, and R. Waugh. 1999. QTL for field resistance to late blight in potato are strongly correlated with maturity and vigour. Molecular Breeding 5: 387–398.

    Article  CAS  Google Scholar 

  • Colton, L.M., H.I. Groza, S.M. Wielgus, and J. Jiang. 2006. Marker-assisted selection for the broad-spectrum potato late blight resistance conferred by gene RB derived from a wild potato species. Crop Science 46: 589–594.

    Article  CAS  Google Scholar 

  • Ewing, E.E., I. Simko, C.D. Smart, M.W. Bonierbale, E.S.G. Mizubuti, G.D. May, and W.E. Fry. 2000. Genetic mapping from field tests of qualitative and quantitative resistance to Phytophthora infestans in a population derived from Solanum tuberosum and Solanum berthaultii. Molecular Breeding 6: 25–36.

    Article  CAS  Google Scholar 

  • Fry, W.E. 2008. Phytophthora infestans: the plant (and R gene) destroyer. Molecular Plant Pathology 9: 385–402.

    Article  PubMed  Google Scholar 

  • Halterman, D., L.C. Kramer, S. Weilgus and J. Jiang. 2008. Performance of transgenic potato containing the late blight resistance gene RB. Plant Disease.

  • Kirk, W.W., K.J. Felcher, D.S. Douches, B.A. Niemira, and R. Hammerschmidt. 2001. Susceptibility of potato (Solanum tuberosum L.) foliage and tubers to the US8 genotype of Phytophthora infestans. American Journal of Potato Research 78: 319–322.

    Article  Google Scholar 

  • Kuhl, J.C., R.E. Hanneman Jr., and M.J. Havey. 2001. Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum. Molecular Genetics and Genomics 265: 977–985.

    Article  CAS  PubMed  Google Scholar 

  • Liu, Z. and D. Halterman. 2006. Identification and characterization of RB orthologous genes from the late blight resistant wild potato species Solanum verrucosum. Physiological and Molecular Plant Pathology 69: 230–239.

    Article  CAS  Google Scholar 

  • Malcolmson, J.F. and W. Black. 1966. New R genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica 15: 199–203.

    Article  Google Scholar 

  • Naess, S.K., J.M. Bradeen, S.M. Wielgus, G.T. Haberlach, J.M. McGrath, and J.P. Helgeson. 2000. Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theoretical and Applied Genetics 101: 697–704.

    Article  CAS  Google Scholar 

  • Oberhagemann, P., C. Chatot-Balandras, R. Schäfer-Pregl, D. Wegener, C. Palomino, F. Salamini, E. Bonnel, and C. Gebhardt. 1999. A genetic analysis of quantitative resistance to late blight in potato: towards marker-assisted selection. Molecular Breeding 5: 399–415.

    Article  CAS  Google Scholar 

  • Park, T.-H., J. Gros, A. Sikkema, V.G.A.A. Vleeshouwers, M. Muskens, S. Allefs, E. Jacobsen, R.G.F. Visser, and E. van der Vossen. 2005a. The late blight resistance locus Rpi-blb3 from Solanum bulbocastanum belongs to a major late blight R gene cluster on chromosome 4 of potato. Molecular Plant-Microbe Interactions 7: 722–729.

    Article  Google Scholar 

  • Park, T.-H., V.G.A.A. Vleeshouwers, J.-B. Kim, R.C.B. Hutten, and R.G.F. Visser. 2005b. Dissection of foliage and tuber late blight resistance in mapping populations of potato. Euphytica 143: 75–83.

    Article  Google Scholar 

  • Platt, H.W. and G.H. Tai. 1998. Relationship between resistance to late blight in potato foliage and tubers of cultivars and breeding selections with different resistance levels. American Journal of Potato Research 75: 173–178.

    Article  Google Scholar 

  • Sandbrink, J.M., L.T. Colon, P.J.C.C. Wolters, and W.J. Stiekema. 2000. Two related genotypes of Solanum microdontum carry different segregating alleles for field resistance to Phytophthora infestans. Molecular Breeding 6: 215–225.

    Article  CAS  Google Scholar 

  • Simko, I., S. Costanzo, V. Ramanjulu, B.J. Christ, and K.G. Haynes. 2006. Mapping polygenes for tuber resistance to late blight in a diploid Solanum phureja X S. stenotomum hybrid population. Plant Breeding 125: 385–389.

    Article  Google Scholar 

  • Simmonds, N.W. and R.L. Wastie. 1987. Assessment of horizontal resistance to late blight of potatoes. Annals of Applied Biology 111: 213–221.

    Article  Google Scholar 

  • Song, J.Q., J.M. Bradeen, S.K. Naess, J.A. Raasch, S.M. Wielgus, G.T. Haberlach, J. Liu, H.H. Kuang, S. Austin-Phillips, C.R. Buell, J.P. Helgeson, and J.M. Jiang. 2003. Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proceedings of the National Academy of Sciences of the United States of America 100: 9128–9133.

    Article  CAS  PubMed  Google Scholar 

  • Stewart, H.E., R.L. Wastie, J.E. Bradshaw, and J. Brown. 1992. Inheritance of resistance to late blight in foliage and tubers of progenies from parents differing in resistance. Potato Research 35: 313–319.

    Article  Google Scholar 

  • Stewart, H.E., J.E. Bradshaw, and R.L. Wastie. 1994. Correlation between resistance to late blight in foliage and tubers in potato clones from parents of contrasting resistance. Potato Research 37: 429–434.

    Article  Google Scholar 

  • Świeżyński, K.M., L. Domański, H. Zarzycka, and E. Zimnoch-Guzowska. 2000. The reaction of potato differentials to Phytophthora infestans isolates collected in nature. Plant Breeding 119: 119–126.

    Article  Google Scholar 

  • Umaerus, V. and M. Umaerus. 1994. Inheritance of resistance to late blight. In Potato genetics, ed. J.E. Bradshaw and G.R. Mackay, 365–401. Wallingford: CAB International.

    Google Scholar 

  • van der Vossen, E., A. Sikkema, B.T.L. Hekkert, J. Gros, P. Stevens, M. Muskens, D. Wouters, A. Pereira, W. Stiekema, and S. Allefs. 2003. An ancient R gene from the wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato. Plant Journal 36: 867–882.

    Article  PubMed  Google Scholar 

  • van der Vossen, E.A.G., J. Gros, A. Sikkema, M. Muskens, D. Wouters, P. Wolters, A. Pereira, and S. Allefs. 2005. The Rpi-blb2 gene from Solanum bulbocastanum is an Mi-1 gene homolog conferring broad-spectrum late blight resistance in potato. Plant Journal 44: 208–222.

    Article  PubMed  Google Scholar 

  • Vleeshouwers, V.G., W. van Dooijeweert, F. Govers, S. Kamoun, and L.T. Colon. 2000. The hypersensitive response is associated with host and nonhost resistance to Phytophthora infestans. Planta 210: 853–864.

    Article  CAS  PubMed  Google Scholar 

  • Vleeshouwers, V.G.A.A., H. Rietman, P. Krenek, N. Champouret, C. Young, S.-K. Oh, M. Wang, K. Bouwmeester, B. Vosman, R.G.F. Visser, E. Jacobsen, F. Govers, S. Kamoun, and E.A.G. Van der Vossen. 2008. Effector Genomics Accelerates Discovery and Functional Profiling of Potato Disease Resistance and Phytophthora Infestans Avirulence Genes. PLoS ONE 3: e2875.

    Article  PubMed  Google Scholar 

  • Wastie, R.L. 1991. Breeding for resistance. In Phytophthora infestans, The cause of late blight in Potato, Advances in plant pathology, ed. D.S. Ingram and P.H. Williams, 193–224. London: Academic.

    Google Scholar 

Download references

Acknowledgments

We would like to thank Sarah Stephenson for expert technical assistance. Salaries and research support were provided by the United States Department of Agriculture and the University of Wisconsin Graduate School.

Disclaimer

This article reports the results of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

Author information

Authors and Affiliations

  1. Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, 53706-1598, USA

    Zhenyu Liu & Dennis Halterman

  2. Vegetable Crops Research Unit, USDA-ARS, 1630 Linden Drive, Madison, WI, 53706-1598, USA

    Dennis Halterman

Authors
  1. Zhenyu Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dennis Halterman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dennis Halterman.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, Z., Halterman, D. Different Genetic Mechanisms Control Foliar and Tuber Resistance to Phytophthora infestans in Wild Potato Solanum verrucosum . Am. J. Pot Res 86, 476–480 (2009). https://doi.org/10.1007/s12230-009-9103-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12230-009-9103-1

Keywords

Search

Navigation