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Impairment of Cronobacter sakazakii and Listeria monocytogenes biofilms by cell-free preparations of lactobacilli of goat milk origin

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Abstract

Biofilm-associated bacterial infections represent one of the major threats to modern medical treatments. Bacteria encased in biofilm matrix are more resistant towards antimicrobials and thus the capability of microbes to persist and nurture in a biofilm seems to be the foremost aspect of pathogenesis and therapeutic failure. Therefore, there is a pressing demand for new drugs active against microbial biofilms. In the current study, anti-biofilm potential of Lactobacillus spp. cell-free supernatants (CFSs) against Cronobacter sakazakii and Listeria monocytogenes was characterized using crystal violet staining and MTT assay. CFSs of goat milk origin lactobacilli not only prevented biofilm formation but also disrupted preformed biofilms. Neutralized and heat-treated preparations of Lactobacillus CFSs also inhibited biofilm formation by test pathogens. The results were quantitatively confirmed by light and fluorescent microscopy observations. Biofilms developed under static conditions displayed typical compact microcolonies with uniform distribution over the surface, while upon CFS challenge, biofilms were disrupted with presence of dead cells. These findings highlight the anti-biofilm potency of Lactobacillus spp. strains of goat milk origin and their potential application in food industries.

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Acknowledgements

The authors acknowledge the financial support from the Department of Science and Technology (PDF/2016/003906) and Ministry of Food Processing Industries, Government of India (SERB/MoFPI/026/2015), for carrying out the research work.

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Authors and Affiliations

  1. Department of Dairy Microbiology, College of Dairy Science and Technology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141004, India

    Niharika Singh, Ravinder Kaur, Brij Pal Singh, Namita Rokana, Anil Kumar Puniya & Harsh Panwar

  2. Central University of Haryana, Mahendergarh, Haryana, 123031, India

    Gunjan Goel

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  1. Niharika Singh
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  2. Ravinder Kaur
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  3. Brij Pal Singh
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Correspondence to Harsh Panwar.

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Singh, N., Kaur, R., Singh, B.P. et al. Impairment of Cronobacter sakazakii and Listeria monocytogenes biofilms by cell-free preparations of lactobacilli of goat milk origin. Folia Microbiol 65, 185–196 (2020). https://doi.org/10.1007/s12223-019-00721-3

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