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Bacterial DNA detected on pathologically changed heart valves using 16S rRNA gene amplification

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Abstract

Nowadays, dental diseases are one of the most common illnesses in the world. Some of them can lead to translocation of oral bacteria to the bloodstream causing intermittent bacteraemia. Therefore, a potential association between oral infection and cardiovascular diseases has been discussed in recent years as a result of adhesion of oral microbes to the heart valves. The aim of this study was to detect oral bacteria on pathologically changed heart valves not caused by infective endocarditis. In the study, patients with pathologically changed heart valves were involved. Samples of heart valves removed during heart valve replacement surgery were cut into two parts. One aliquot was cultivated aerobically and anaerobically. Bacterial DNA was extracted using Ultra-Deep Microbiome Prep (Molzym GmbH, Bremen, Germany) followed by a 16S rRNA gene PCR amplification using Mastermix 16S Complete kit (Molzym GmbH, Bremen, Germany). Positive PCR products were sequenced and the sequences were analyzed using BLAST database (http://www.ncbi.nlm.nih/BLAST). During the study period, 41 samples were processed. Bacterial DNA of the following bacteria was detected in 21 samples: Cutibacterium acnes (formerly Propionibacterium acnes) (n = 11; 52.38% of patients with positive bacterial DNA detection), Staphylococcus sp. (n = 9; 42.86%), Streptococcus sp. (n = 1; 4.76%), Streptococcus sanguinis (n = 4; 19.05%), Streptococcus oralis (n = 1; 4.76%), Carnobacterium sp. (n = 1; 4.76%), Bacillus sp. (n = 2; 9.52%), and Bergeyella sp. (n = 1; 4.76%). In nine samples, multiple bacteria were found. Our results showed significant appearance of bacteria on pathologically changed heart valves in patients with no symptoms of infective endocarditis.

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Funding

This study was supported by the Charles University Research Fund - PROGRES (project number Q39), National Sustainability Program LO 1503 provided by Ministry of Education, Youth and Sports of the Czech Republic, by the grant no. 15-32727A provided by the Czech Health Research Council and by Conceptual Development of Research Organization (Faculty Hospital in Pilsen - FNPl, 00669806).

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Authors and Affiliations

  1. Department of Stomatology, Faculty of Medicine and University Hospital in Pilsen, Charles University, alej Svobody 80, 304 60, Plzeň-Lochotín, Czech Republic

    Miroslava Chalupova & Hana Hecova

  2. Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Plzen, Czech Republic

    Miroslava Chalupova, Anna Skalova, Dana Kralova & Jaroslav Hrabak

  3. Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Plzen, Czech Republic

    Anna Skalova, Lenka Geigerova, Dana Kralova & Jaroslav Hrabak

  4. Department of Cardiac Surgery, University Hospital in Pilsen, Charles University, Plzen, Czech Republic

    Tomas Hajek

  5. Czech Statistical Office, Prague, Czech Republic

    Pavel Liska

  6. Department of Surgery, Faculty of Medicine and University Hospital in Pilsen, Charles University, Plzen, Czech Republic

    Jiri Molacek

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  1. Miroslava Chalupova
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  2. Anna Skalova
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  3. Tomas Hajek
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  4. Lenka Geigerova
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  7. Hana Hecova
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  9. Jaroslav Hrabak
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Correspondence to Miroslava Chalupova.

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The study was approved by the Local Committee on Medical Ethics and the study was performed in agreement with the national law.

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Chalupova, M., Skalova, A., Hajek, T. et al. Bacterial DNA detected on pathologically changed heart valves using 16S rRNA gene amplification. Folia Microbiol 63, 707–711 (2018). https://doi.org/10.1007/s12223-018-0611-6

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  • DOI: https://doi.org/10.1007/s12223-018-0611-6

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