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Lipid accumulation by oleaginous and non-oleaginous yeast strains in nitrogen and phosphate limitation

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Abstract

We investigated the possibility of utilizing both oleaginous yeast species accumulating large amounts of lipids (Yarrowia lipolytica, Rhodotorula glutinis, Trichosporon cutaneum, Candida sp.) and traditional biotechnological non-oleaginous ones characterized by high biomass yield (Kluyveromyces polysporus, Torulaspora delbrueckii, Saccharomyces cerevisiae) as potential producers of biofuel-utilizable and nutritionally valuable lipids. The main objective was to increase lipid accumulation by increasing C/P ratio together with higher C/N ratio, while maintaining high biomass yield. The C/N ratio of 30 was found to lead to higher biomass content and the total lipid content increased significantly with higher C/P ratio. With higher ratios of both C/N and C/P, the content of monounsaturated fatty acids (FAs) in cell lipids increased while polyunsaturated FAs decreased. Oleaginous yeast species had a lower proportion of unsaturated FAs (approx. 80 %) than non-oleaginous strains (approx. 90 %). At a C/N ratio of 30 and C/P ratio 1043, T. cutaneum produced a high amount of ω-6 unsaturated linoleic acid, the precursor of some prostaglandins, leukotrienes, and thromboxanes, while Candida sp. and K. polysporus accumulated a high content of palmitoleic acid.

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Acknowledgments

The research was supported by Czech Science Foundation (GACR) project P503 14-00227S.

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Authors and Affiliations

  1. Department of Biotechnology, University of Chemistry and Technology, Technická 5, CZ-166 28, Prague 6, Czech Republic

    Irena Kolouchová, Olga Maťátková, Jan Masák & Tomáš Řezanka

  2. Institute of Microbiology, CAS, Vídeňská 1083, 142 20, Prague, Czech Republic

    Karel Sigler & Tomáš Řezanka

Authors
  1. Irena Kolouchová
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  2. Olga Maťátková
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  3. Karel Sigler
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  4. Jan Masák
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  5. Tomáš Řezanka
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Correspondence to Olga Maťátková.

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Kolouchová, I., Maťátková, O., Sigler, K. et al. Lipid accumulation by oleaginous and non-oleaginous yeast strains in nitrogen and phosphate limitation. Folia Microbiol 61, 431–438 (2016). https://doi.org/10.1007/s12223-016-0454-y

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  • DOI: https://doi.org/10.1007/s12223-016-0454-y

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