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Characteristics and genetic diversity of bioluminescent Shewanella woodyi strains isolated from the Gulf of Izmir, Turkey

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Abstract

The purpose of this study was to isolate bioluminescent strains and to phenotypically and biochemically identify them based on the 16S rRNA gene sequence. 16S rRNA gene sequence analysis of the 11 isolates revealed that they belonged to Shewanella woodyi. Nevertheless, they were determined to exhibit various growth characteristics, enzymatic activities, assimilation of carbon and nitrogen sources, and different characteristics in antibiotic resistance profiles, and also, it was determined that different growth conditions affect the amount of biofilm. Pulsed-field gel electrophoresis (PFGE) analysis of S. woodyi strains performed with SmaI and NotI restriction enzymes revealed that they exhibited restriction fragment pattern homology ranging from 56 to 89 % and from 82 to 94 %, respectively. As a result, PFGE analysis of the genome S. woodyi (as the first record) revealed that although these strains inhabiting the Gulf of Izmir exhibit common characteristics, they also have high levels of genomic polymorphism.

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Acknowledgments

The authors thank Izmir Institute of Technology, Biotechnology and Bioengineering Research and Application Center for molecular assays. This work was supported by BAP Project (2007 FEN 024), Ege University.

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  1. Ege University, Faculty of Science, Department of Biology, Basic and Industrial Microbiology Section, 35100, Bornova, Izmir, Turkey

    Esra Ersoy Omeroglu & Ismail Karaboz

  2. Mehmet Akif Ersoy University, Central Research Laboratory, 15000, Ortulu, Burdur, Turkey

    Mert Sudagidan

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Ersoy Omeroglu, E., Karaboz, I. & Sudagidan, M. Characteristics and genetic diversity of bioluminescent Shewanella woodyi strains isolated from the Gulf of Izmir, Turkey. Folia Microbiol 59, 79–92 (2014). https://doi.org/10.1007/s12223-013-0269-z

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