Abstract
Biochemical and serological profiles of isolates of Plesiomonas shigelloides were assayed using standard procedures in isolates from various clinical samples. Seventy-four isolates, including P. shigelloides type strain, were further characterized by MALDI-TOF MS using 3-methoxy-4-hydroxycinnamic acid as matrix. Multiple ions in the 3- to 12-kDa mass range were found in the spectra of each strain, from which the “species-identifying” unique biomarker ions were identified. After creating the species-specific patterns, a spectral database was generated for reliable, rapid, reproducible and accurate identification of Plesiomonas strains. The classical strain description (biochemical and serological) was thus complemented with the metabolic (proteomic) characterization.
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Kolínská, R., Dřevínek, M., Aldová, E. et al. Identification of Plesiomonas spp.: Serological and MALDI-TOF MS methods. Folia Microbiol 55, 669–672 (2010). https://doi.org/10.1007/s12223-010-0109-3
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DOI: https://doi.org/10.1007/s12223-010-0109-3