Abstract
Activity of some enzymes of a Pb-adapted strain of Rhizopus arrhizus augmented significantly during bioaccumulation of Pb from a chemically defined medium. The mycelium of a Pb-adapted strain exposed to 1 μg/mL Pb in a defined medium for 10 d at 28 ± 2 °C exhibited, relative to a wild-type strain, increased activities of antioxidant enzymes, i.e. SOD, CAT and GPX and of enzymes like AP and PPO involved in defense against pathogens. Another response is a significant increase in the cellular thiol content after 4 d. The responses to Pb exposure thus include an increase in the antioxidant and anti-pathogen defense, and an increased metal-chelating ability.
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Abbreviations
- AP:
-
acid phosphatase
- CAT:
-
catalase
- GPX:
-
glutathione peroxidase
- NBT:
-
nitroblue tetrazolium
- PPO:
-
polyphenol oxidase
- SOD:
-
superoxide dismutase
- WT:
-
wild-type (strain)
References
Azevedo M.M., Carvalho A., Pascoal C., Rodrigues F., Cassio F.: Responses of antioxidant defenses to Cu and Zn stress in two aquatic fungi. Sci.Total Environ.377, 233–243 (2007).
Basumajumdar A., Bhattacharyya S., Pal T.K.: Some trace elements enhance biosorption of lead by Rhizopus arrhizus. J.Ind. Chem.Soc.81, 410–414 (2004).
Bhattacharyya S., Pal T.K., Basumajumdar A., Banik A.K.: Biosorption of heavy metals by Rhizopus arrhizus and Aspergillus niger. J.Ind.Chem.Soc.79, 747–751 (2002).
Chattopadhyay M., Banik A.K., Raychaudhuri S.: Production and purification of lipase by a mutant strain of Rhizopus arrhizus. Folia Microbiol.44, 37–40 (1999).
Gonnety J.T., Niamke S., Fanlet B.M., N’Guessan Kouadio E.J., Kouame L.P.: Purification and characterization of three lowmolecular-weight acid phosphatases from peanut (Arachis hypogaea) seedlings. Afr.J.Biotechnol.5, 35–44 (2006).
Guelfi A., Azevedo R.A., Lea P.J., Molina S.M.G.: Growth inhibition of the filamentous fungus Aspergillus nidulans by cadmium: an antioxidant enzyme approach. J.Gen.Appl.Microbiol.49, 63–73 (2003).
Isobe K., Inoue N., Takamatsu Y., Kamada K., Wakao N.: Production of catalase by fungi growing at low pH and high temperature. J.Biosci.Bioeng.101, 73–76 (2006).
Li Q., Mcneil B., Harvey L.M.: Adaptive response to oxidative stress in the filamentous fungus Aspergillus niger B1-D. Free Rad. Biol.Med.44, 394–402 (2008).
Liszewska F., Blaszczyk A., Sirko A.: Modification of non-protein thiols contents in transgenic tobacco plants producing bacterial enzymes of cysteine biosynthesis pathway. Acta Biochim.Pol.48, 647–656 (2001).
Mayer A.M.: Polyphenol oxidase in plants and fungi: going places? — review. Phytochemistry67, 2318–2331 (2006).
Nagai T., Yukimoto T., Suzuki N.: Glutathione peroxidase from the liver of Japanese sea bass Lateolabrax japonicus. Z.Naturforsch.57c, 172–176 (2002).
Todorova D., Nedeva D., Abrashev R., Tsekova K.: Cd(II) stress response during the growth of Aspergillus niger B77. J.Appl. Microbiol.104, 178–184 (2008).
Toralles R.P., Vendruscolo J.L., Vendruscolo C.T., del Pino F.A.B., Antunes P.L.: Properties of polyphenoloxidase and peroxidase from Granada clingstone peaches. Braz.J.Food Technol.8, 233–242 (2005).
Tsekova K., Todorova K.: Copper(II) accumulation and superoxide dismutase activity during growth of Aspergillus niger B-77. Z.Naturforsch.57c, 319–322 (2002).
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Bhattacharyya, S., Pal, T.K. & Basumajumdar, A. Modulation of enzyme activities of a lead-adapted strain of Rhizopus arrhizus during bioaccumulation of lead. Folia Microbiol 54, 505–508 (2009). https://doi.org/10.1007/s12223-009-0072-z
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DOI: https://doi.org/10.1007/s12223-009-0072-z