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Live and heat-inactivated lactobacilli from feces inhibit Salmonella typhi and Escherichia coli adherence to caco-2 cells

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Abstract

A quantitative approach has been proposed to evaluate the competitive inhibition of Escherichia coli and Salmonella typhi by live and heat-inactivated laboratory isolated Lactobacillus sp. on adhesion to monolayer of Caco-2 cells. Three species of Lactobacillus (L. casei, L. acidophilus, L. agilis) isolated from human neonate feces and two commercial probiotic strains (L. casei, L. acidophilus) have been compared for probiotic activity. All lactobacilli were able to attach to the Caco-2 cells, however, the degree of adhesion was bacterial strain-dependent. The adhesion indices of the two commercial probiotic strains were not significantly different from the values obtained for the other two similar fecal strains (p > 0.01). The inhibition of attachment of the pathogenic bacteria by inactivated cells of fecal L. acidophilus was examined and compared to the results of live bacteria. The inhibition pattern was similar for live and heat-inactivated L. acidophilus (p > 0.01). The number of attached pathogenic bacteria to the Caco-2 cells decreased when the number of L. acidophilus increased from 106 to 109 CFU/mL. The heat-inactivated L. acidophilus displayed similar probiotic activity compared to the live bacteria.

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Abbreviations

CFU:

colony forming unit

MRS broth:

Man-Rogosa-Sharp broth

SCDB:

soybean-casein digest broth

EMB agar:

eosin-methylene blue agar

PBS:

phosphate-buffered saline

SS agar:

Salmonella-Shigella agar

References

  • Baron E.J., Finegold S.M.:Bailey & Scott’s Diagnostic Microbiology, 8th ed., pp. 323–332. C.V. Mosby Co., St. Louis (USA) 1990.

    Google Scholar 

  • Benno Y., Mitsuoka T.: Impact of Bifidobacterium longum on human fecal microflora. Microbiol.Immun. 36, 683–694 (1992).

    CAS  Google Scholar 

  • ÄŚepeljnik T., Lah B., Narat M., Marinšek-Logar R.: Adaptation of adhesion test using Caco-2 cells of anaerobic bacterium Pseudobutyrivibrio xylanivorans, a probiotic candidate. Folia Microbiol. 52, 367–373 (2007).

    Article  Google Scholar 

  • Coconnier M.H., Bernet M.F., Chauviere G., Servin A.L.: Adhering heat-killed human Lactobacillus acidophilus, strain LB, inhibits the process of pathogenicity of diarrheagenic bacteria in cultured human intestinal cells. J.Diarrh.Dis.Res. 11, 235–242 (1993).

    CAS  Google Scholar 

  • Drago L., Gismondo M.R., Lombardi A., de HaĂ©n C., Gozzini L.: Inhibition of in-vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin. FEMS Microbiol.Lett. 153, 455–463 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Ebringer L., FerenÄŤĂ­k M., KrajÄŤoviÄŤ J.: Beneficial health effects of milk and fermented dairy products — review. Folia Microbiol. 53, 378–394 (2008).

    Article  CAS  Google Scholar 

  • Elliot S.E., Buret A., Mcknight W., Miller M.J.S., Wallace J.L.: Bacteria rapidly colonize and modulate healing of gastric ulcers in rats. Am.J.Physiol. 275, G425–G432 (1998).

    Google Scholar 

  • FAO/WHO: Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. Report of a joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria, Cordoba (Argentina) 2001.

  • Fazeli M.R., Shahverdi A.R., Sedaghat B., Jamalifar H., Samadi N.: Sourdough-isolated Lactobacillus fermentum as a potent anti-mould preservative of a traditional Iranian bread. Eur.Food Res.Technol.218, 554–556 (2004).

    Article  CAS  Google Scholar 

  • Fazeli M.R., Toliyat T., Samadi N., Hajjaran S., Jamalifar H.: Viability of Lactobacillus acidophilus in various vaginal tablet formulations. Daru14, 172–178 (2006).

    CAS  Google Scholar 

  • Fuller R.: Probiotics in man and animals. J.Appl.Bacteriol. 66, 365–378 (1989).

    PubMed  CAS  Google Scholar 

  • Juven B.J., Schved F., Linder P.: Antagonistic compounds produced by a chicken intestinal strain of Lactobacillus acidophilus. J.Food Prot.55, 157–161 (1992).

    CAS  Google Scholar 

  • Kato I., Endo K., Yokokura T.: Effects of oral administration of Lactobacillus casei on antitumor responses induced by tumor resection in mice. Internat.J.Immunol.16, 29–36 (1994).

    Article  CAS  Google Scholar 

  • Klaenhammer T.R.: Probiotic bacteria: today and tomorrow. J.Nutr.130, 415S–416S (2000).

    PubMed  CAS  Google Scholar 

  • Koninkx J.F.J.G., Malago J.J.: The protective potency of probiotic bacteria and their microbial products against enteric infections — review. Folia Microbiol.53, 189–194 (2008).

    Article  CAS  Google Scholar 

  • Krieg R.N., Holt J.G.: Bergey’s Manual of Systematic Bacteriology, Vol. 2., pp. 1208–1234. Williams and Wilkins, Baltimore 1984.

    Google Scholar 

  • Lee Y.K., Salminen S.: The coming of age of probiotics. Trends Food Sci.Technol.6, 241–244 (1995).

    Article  Google Scholar 

  • Lidbeck A., Gustafasson J.A., Nord C.: Impact of Lactobacillus acidophilus supplements on the human oropharyngeal and intestinal microflora. Scand.J.Infect.Dis.19, 531–537 (1987).

    Article  PubMed  CAS  Google Scholar 

  • Ouwehand A.C., Salminen S.J.: The health effects of cultured milk products with viable and non-viable bacteria. Internat.Dairy J.8, 749–758 (1998).

    Article  Google Scholar 

  • Perdigon G., Alvarez S., Gobbato N., de Budeguer M.V., de Ruiz Holgado A.A.P.: Comparative effect of the adjuvant capacity of Lactobacillus casei and lipopolysaccharide on the intestinal secretary antibody response and resistance to Salmonella infection in mice. Food Agric.Immunol.7, 283–294 (1995).

    Article  CAS  Google Scholar 

  • Rigon-Zimmer K., MulliĂ© C., Tir-Touil-Meddah A., Buisson P., LĂ©kĂ© L., Canarelli J.P.: Impact of colostomy on intestinal microflora and bacterial translocation in young rats fed with heat-killed Lactobacillus acidophilus strain LB. Folia Microbiol.53, 89–93 (2008).

    Article  CAS  Google Scholar 

  • Salminen S., Ouwehand A., Benno Y., Lee Y.K.: Probiotics: how should they be defined?. Food Sci.Technol.10, 107–110 (1999).

    CAS  Google Scholar 

  • Saxelin M., Pessi T., Salminen S.: Fecal recovery following oral administration of Lactobacillus strain GG (ATCC 53103) in gelatin capsules to healthy volunteers. Internat.J.Food Microbiol.25, 199–203 (1995).

    Article  CAS  Google Scholar 

  • Schiffrin E.J., Brassart D., Servin A.L., Rochat F., Donnet-Hughes A.: Immune modulation of blood leukocytes in humans by lactic acid bacteria: criteria for strain selection. Am.J.Clin.Nutr.66, 515S–520S (1997).

    PubMed  CAS  Google Scholar 

  • Servin A.L.: Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiol.Rev.28, 405–440 (2004).

    Article  PubMed  CAS  Google Scholar 

  • Tamura N., Norimoto M., Yoshida K., Hirayama C., Nakai R.: Alteration of fecal bacterial flora following oral administration of bifidobacterial preparation. Gasteroenterol.Japan18, 17–24 (1983).

    Google Scholar 

  • Walencka E., Różalska S., Sadowska B., Różalska B.: The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation. Folia Microbiol.53, 861–866 (2008).

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Toxicology-Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 14155/6451, Tehran, Iran

    S. N. Ostad, A. A. Salarian & M. H. Ghahramani

  2. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, 14155/6451, Tehran, Iran

    M. R. Fazeli, N. Samadi & H. Jamalifar

Authors
  1. S. N. Ostad
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  2. A. A. Salarian
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  3. M. H. Ghahramani
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  4. M. R. Fazeli
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  5. N. Samadi
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  6. H. Jamalifar
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Correspondence to S. N. Ostad.

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Ostad, S.N., Salarian, A.A., Ghahramani, M.H. et al. Live and heat-inactivated lactobacilli from feces inhibit Salmonella typhi and Escherichia coli adherence to caco-2 cells. Folia Microbiol 54, 157–160 (2009). https://doi.org/10.1007/s12223-009-0024-7

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  • DOI: https://doi.org/10.1007/s12223-009-0024-7

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