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Production and regulation of lignocellulose-degrading enzymes of Poria-like wood-inhabiting basidiomycetes

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Abstract

The wood-decomposing fungal species Antrodia macra, A. pulvinascens, Ceriporiopsis aneirina, C. resinascens and Dichomitus albidofuscus were determined for production of laccase (LAC), Mn peroxidase (MnP), lignin peroxidase (LiP), endo-l,4-P-β-glucanase, endo-l,4-β-xylanase, cellobiohydrolase, 1,4-β-glucosidase and 1,4-β-xylosidase. The results confirmed the brown-rot mode of Antrodia spp. which did not produce the activity of LAC and MnP. The remaining species performed detectable activity of both enzymes while no strain produced LiP. Significant inhibition of LAC production by high nitrogen was found in all white-rot species while only MnP of D. albidofuscus was regulated in the same way. The endoglucanase and endoxylanase activities of white-rotting species were inhibited by glucose in the medium while those of Antrodia spp. were not influenced by glucose concentration. The regulation of enzyme activity and bio-mass production can vary even within a single fungal genus.

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Abbreviations

ABTS:

2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)

BRNM:

Herbarium of Botany Department, Moravian Museum, Bmo

CBH:

cellobiohydrolase

DMAB:

3,3-dimethylaminobenzoic acid

EDTA:

1,2-ethylenediaminetetraacetate

HN:

high nitrogen (medium)

ITS:

internal transcribed spacer

LAC:

laccase

LiP:

lignin peroxidase

LN:

low nitrogen (medium)

MBTH:

3-methyl-2-benzothiazolinone hydrazone

MnP:

Mn-peroxidase

MUAF:

Department of Forest Protection and Wildlife Management, Mendel University in Brno

PNPC:

4-nitrophenyl β-d-cellobioside

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Authors and Affiliations

  1. Faculty of Forestry and Wood Technology, Mendel University of Agriculture and Forestry in Brno, 61300, Brno, Czech Republic

    M. Tomšovský

  2. Laboratory of Biochemistry of Wood-Rotting Fungi, Institute of Microbiology of the ASCR, v.v.i, 142 20, Prague, Czech Republic

    P. Popelářová & P. Baldrian

Authors
  1. M. Tomšovský
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  2. P. Popelářová
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  3. P. Baldrian
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Correspondence to M. Tomšovský.

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Tomšovský, M., Popelářová, P. & Baldrian, P. Production and regulation of lignocellulose-degrading enzymes of Poria-like wood-inhabiting basidiomycetes. Folia Microbiol 54, 74–80 (2009). https://doi.org/10.1007/s12223-009-0011-z

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  • DOI: https://doi.org/10.1007/s12223-009-0011-z

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