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Production of 3-indolylacetic acid in root nodules and culture by a Rhizobium species isolated from root nodules of the leguminous pulse Phaseolus mungo

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Abstract

The root nodules of Phaseolus mungo (a herbaceous leguminous pulse) contained a high amount of 3-indolylacetic acid (IAA). A tryptophan pool present in the nodule might play the role of precursor for IAA production. From the root nodule a Rhizobium sp. was isolated. The symbiont produced a large amount of IAA (142 μg/mL) from L-tryptophan-supplemented basal medium. The production of IAA by the symbiont was much increased over the control when a l-tryptophan (2 mg/mL) supplemented C-free mineral medium was enriched with mannitol (1 %), l-asparagine (0.3 %) and thiamine hydrochloride (1 μg/mL). The possible role of the rhizobial production of IAA on the rhizobia-legume symbiosis is discussed.

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Abbreviations

IAA:

3-indolylacetic acid

YEM:

yeast extract mineral medium

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Authors and Affiliations

  1. Department of Botany, Burdwan University, Golapbag, Burdwan, 713 104, West Bengal, India

    S. Ghosh, T. K. Maiti & P. S. Basu

  2. Department of Botany, Kalyani University, Kalyani, 741 235, Nadia, West Bengal, India

    C. Sengupta

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  1. S. Ghosh
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  2. C. Sengupta
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  3. T. K. Maiti
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  4. P. S. Basu
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Correspondence to T. K. Maiti.

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Ghosh, S., Sengupta, C., Maiti, T.K. et al. Production of 3-indolylacetic acid in root nodules and culture by a Rhizobium species isolated from root nodules of the leguminous pulse Phaseolus mungo . Folia Microbiol 53, 351–355 (2008). https://doi.org/10.1007/s12223-008-0054-6

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