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Mechanical and biological properties of silk fibroin/carbon nanotube nanocomposite films

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Abstract

Multi-walled carbon nanotubes (MWCNTs) were homogeneously dispersed in silk fibroin (SF) solutions at different compositions, and a simple solvent-casting method was used to fabricate SF/MWCNT films. Structure, viscosity, and mechanical properties of the SF/MWCNT nanocomposites were characterized by FTIR (Fourier Transform Infrared Spectroscopy), viscometry, and tensile testing. Fibroblast cells were used to examine cell viability and attachment to nanocomposite films. Compared to a pure SF film, adding just 0.5 % (w/w) of MWCNT to the SF matrix could enhance the Young’s modulus and ultimate tensile strength by approximately 24 % and 39 %, respectively. In addition, with increasing MWCNTs content, the percentage of β-sheet structure increased significantly. When the content of MWCNTs increased from 0 to 1 % (w/w), the β-sheet content improved from 17.8 % to 53.6 %. Furthermore, adding up to 1 % (w/w) MWCNT increased the viscosity of the SF solution ten fold. From a biological point of view, no toxic effect was observed on fibroblast cells in the presence of MWCNTs after 3 and 7 days. This investigation suggests that SF/MWCNT nanocomposite films with improved mechanical properties and biocompatibility are well-suited as novel biomaterials.

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References

  1. A. K. Mohanty, M. Misra, and G. Hinrichsen, Macromol. Mater. Eng., 276, 1 (2000).

    Article  Google Scholar 

  2. R. A. MacDonald, B. F. Laurenzi, G. Viswanathan, P. M. Ajayan, and J. P. Stegemann, Biomed. Mater. Res. A., 74, 489 (2005).

    Article  Google Scholar 

  3. S. Iijima, Nature, 354, 56 (1991).

    Article  CAS  Google Scholar 

  4. M. Shokrieh and R. Rafiee, Mech. Compo. Mater., 46, 155 (2010).

    Article  CAS  Google Scholar 

  5. A. A. Balandin, Nat. Mater., 10, 569 (2011).

    Article  CAS  Google Scholar 

  6. R. A. MacDonald, C. M. Voge, M. Kariolis, and J. P. Stegemann, Acta Biomater., 4, 1583 (2008).

    Article  CAS  Google Scholar 

  7. S. Agarwal, X. Zhou, F. Ye, Q. He, G. C. Chen, J. Soo, F. Boey, H. Zhang, and P. Chen, Langmuir, 26, 2244 (2010).

    Article  CAS  Google Scholar 

  8. S. K. Misra, F. Ohashi, S. P. Valappil, J. C. Knowles, I. Roy, S. R. P. Silva, V. Salih, and A. R. Boccaccini, Acta Biomater., 6, 735 (2010).

    Article  CAS  Google Scholar 

  9. J. E. Tercero, S. Namin, D. Lahiri, K. Balani, N. Tsoukias, and A. Agarwal, Mater. Sci. Eng. C-Mater. Biol. Appl., 29, 2195 (2009).

    Article  CAS  Google Scholar 

  10. A. Lobo, E. Antunes, A. Machado, C. Pacheco-Soares, V. Trava-Airoldi, and E. Corat, Mater. Sci. Eng. C-Mater. Biol. Appl., 28, 264 (2008).

    Article  CAS  Google Scholar 

  11. B. S. Harrison and A. Atala, Biomaterials, 28, 344 (2007).

    Article  CAS  Google Scholar 

  12. G. H. Altman, F. Diaz, C. Jakuba, T. Calabro, R. L. Horan, J. Chen, H. Lu, J. Richmond, and D. L. Kaplan, Biomaterials, 24, 401 (2003).

    Article  CAS  Google Scholar 

  13. U. J. Kim, J. Park, H. J. Kim, M. Wada, and D. L. Kaplan, Biomaterials, 26, 2775 (2005).

    Article  CAS  Google Scholar 

  14. Y. Wang, H. J. Kim, G. Vunjak-Novakovic, and D. L. Kaplan, Biomaterials, 27, 6064 (2006).

    Article  CAS  Google Scholar 

  15. J. Qu, Y. Liu, Y. Yu, J. Li, and J. Luo, Mater. Sci. Eng. CMater. Biol. Appl., 44, 166 (2014).

    Article  CAS  Google Scholar 

  16. J. D. White, S. Wang, A. S. Weiss, and D. L. Kaplan, Acta Biom., 14, S1742-7061-00548-0 (2014).

  17. X. Wang, B. Partlow, J. Liu, Z. Zhang, and B. Su, Acta Biom., 14, S1742-7061-00469-3 (2014).

  18. J. Liu, X. Zhang, H. Pang, B. Liu, and Q. Zou, Biosens. Bioelectron., 15, 31 (2012).

    CAS  Google Scholar 

  19. C. Chi-Shuo, S. Sushant, and L. Catherine, Nanoscale Res. Lett., 7, 126 (2012).

    Article  Google Scholar 

  20. S. Y. Cho, Y. S. Yun, E. S. Kim, M. S. Kim, and H. J. Jin, Nano. Nanotechnol., 5, 801 (2011).

    Article  Google Scholar 

  21. D. Blond, D. N. McCarthy, W. J. Blau, and J. N. Coleman, Biomacromolecules, 8, 3973 (2007).

    Article  CAS  Google Scholar 

  22. A. Jonathan, G. Milind, S. Sachiko, Y. Haihui, and H. Chen-ming, Biomacromolecules, 7, 208 (2006).

    Article  Google Scholar 

  23. A. Fraczek, E. Menaszek, C. Paluszkiewicz, and M. Blazewicz, Acta Biomater., 4, 1593 (2008).

    Article  CAS  Google Scholar 

  24. M. Matsuoka, T. Akasaka, Y. Totsuka, and F. Watari, Mat. Sci. Eng. B., 173, 182 (2010).

    Article  CAS  Google Scholar 

  25. S. Sofia, M. B. McCarthy, G. Gronowicz, and D. L. Kaplan, Biomed. Mater., 54, 139 (2001).

    Article  CAS  Google Scholar 

  26. H. Xiao, D. L. Kaplan, and P. Cebe, Macromolecular, 39, 6161 (2006).

    Article  Google Scholar 

  27. Z. Wu, W. Feng, Y. Feng, Q. Liu, X. Xu, T. Sekino, A. Fujii, and M. Ozaki, Carbon, 45, 1212 (2007).

    Article  CAS  Google Scholar 

  28. J. L. Bahr, E. T. Mickelson, M. J. Bronikowski, R. E. Smalley, and J. M. Tour, Chem. Commun., 193 (2001).

    Google Scholar 

  29. C. Tang, T. Zhou, J. Yang, Q. Zhang, F. Chen, Q. Fu, and L. Yang, Colloid Surf. B-Biointerfaces, 86, 189 (2011).

    Article  CAS  Google Scholar 

  30. A. Jonathan, G. Milind, S. Sachiko, and T. Haihui, Biomacromolecules, 7, 208 (2006).

    Article  Google Scholar 

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Authors and Affiliations

  1. Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou, 310058, China

    Caixia Pan, Qifan Xie, Zeyun Hu, Mingying Yang & Liangjun Zhu

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  1. Caixia Pan
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  2. Qifan Xie
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  3. Zeyun Hu
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  4. Mingying Yang
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  5. Liangjun Zhu
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Correspondence to Liangjun Zhu.

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Pan, C., Xie, Q., Hu, Z. et al. Mechanical and biological properties of silk fibroin/carbon nanotube nanocomposite films. Fibers Polym 16, 1781–1787 (2015). https://doi.org/10.1007/s12221-015-5185-1

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  • DOI: https://doi.org/10.1007/s12221-015-5185-1

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