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Poly-3-hydroxybutyrate (PHB) production by Bacillus flexus ME-77 using some industrial wastes

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Abstract

In this study, 50 bacterial strains were screened for polyhydroxyalkanoate (PHA) production. The isolated bacterial strain Bacillus flexus ME-77 was found to be the most potent PHA producer. The extracted PHA was analyzed using NMR techniques, which showed that it is of the polyhydroxybutyrate (PHB) type. The highest PHB production (4.5 g/L) was achieved using 30 g/L sugarcane molasses (SCM) at pH 7.5 and applying 1, 0.2 and 0.03 g/L of phosphate, MgSO4 7H2O and CaCl2, respectively, at 27 °C incubation temperature and 200 rpm agitation speed. The bacterial strain Bacillus flexus ME-77 with its potential could be further explored for cost-effective production of PHAs using low-cost agro-industrial food wastes such as sugarcane molasses as a low-cost sole carbon source.

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Authors and Affiliations

  1. Botany Department, Faculty of Science, Tanta University, Tanta, Egypt

    Mostafa M. El-Sheekh

  2. Natural and Microbial Products Chemistry Department, National Research Centre, Cairo, Egypt

    Mostafa A. El-Abd, Ahmed I. El-Diwany & Abdel-mohsen S. Ismail

  3. Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt

    Thanaa H. Omar

Authors
  1. Mostafa M. El-Sheekh
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  2. Mostafa A. El-Abd
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  3. Ahmed I. El-Diwany
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  4. Abdel-mohsen S. Ismail
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  5. Thanaa H. Omar
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Correspondence to Mostafa M. El-Sheekh.

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El-Sheekh, M.M., El-Abd, M.A., El-Diwany, A.I. et al. Poly-3-hydroxybutyrate (PHB) production by Bacillus flexus ME-77 using some industrial wastes. Rend. Fis. Acc. Lincei 26, 109–119 (2015). https://doi.org/10.1007/s12210-014-0368-z

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