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Fecal Microbiota Succession of Piglets from Birth to Post-weaning by 454 Pyrosequencing Analysis

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Abstract

The objective of this study was to describe the fecal microbiota succession of piglets from birth to post-weaning by pyrosequencing. The dominant bacterial phyla were Bacteroidetes, Firmicutes, Proteobacteria, and Fusobacteria, although the composition of the fecal microbiota changed as the piglets grew. Bacteroides, Parabacteroides, Prevotella, Lactobacillus, unclassified Lachnospiraceae, Ruminococcus, Oscillospira, Phascolarctobacterium, and Desulfovibrio were the core genera in the feces of piglets and existed from birth to post-weaning. Bacteroides was the most abundant at birth; however, the proportion of Bacteroides decreased as the piglets aged, while the proportion of Prevotella increased until it became the most abundant genus at the post-weaning stage. Principal coordinate analysis indicated that the microbial communities of piglet fecal samples from birth to post-weaning could be separated into four groups according to the similarity of community composition. These corresponded to time points on day 0 (birth); day 7; days 14, 21, and 28 (pre-weaning); and day 35 (post-weaning). This study suggests that the development of the intestinal microbiota in the piglet is a gradual and sequential process. This methodology establishes a basis for broader studies to investigate the microbiota of the piglet intestinal tract.

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Acknowledgements

This work was supported by the Open Funding Project of the Key Laboratory of Systems Bioengineering in Tianjin, Ministry of Education of China (No. 20160110).

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Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Jin Wang, Ye Han & Zhijiang Zhou

  2. Tianjin Institute of Animal Husbandry and Veterinary Research, Tianjin, 300384, China

    Fanrui Meng & Huan Fan

  3. Tianjin Kuntai Environment & Energy Science and Technology Development Co.,Ltd, Tianjin, 300191, China

    Jinzhao Zhao

  4. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, 300072, China

    Huan Fan

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  1. Jin Wang
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  2. Ye Han
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  3. Fanrui Meng
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  4. Jinzhao Zhao
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  5. Zhijiang Zhou
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  6. Huan Fan
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Correspondence to Zhijiang Zhou or Huan Fan.

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Wang, J., Han, Y., Meng, F. et al. Fecal Microbiota Succession of Piglets from Birth to Post-weaning by 454 Pyrosequencing Analysis. Trans. Tianjin Univ. 23, 211–220 (2017). https://doi.org/10.1007/s12209-017-0045-2

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  • DOI: https://doi.org/10.1007/s12209-017-0045-2

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