Abstract
Micro-stereolithography (MSTL) among various 3D printing technologies reaches high resolution by using a focused laser beam, and therefore it can be used to fabricate objects that have feature sizes of tens to hundreds of micrometers. To fabricate a scaffold for the tissue engineering, we used a Blu-ray based MSTL system which is simpler and more compact than the conventional MSTL system. We selected a biodegradable photopolymer, Poly (propylene fumarate)/diethyl fumarate (PPF/DEF), as the construction material, and progressed post-curing to strengthen the fabricated scaffold. We seeded MC3T3-E1 pre-osteoblasts on the fabricated PPF/DEF 3D scaffolds and cultured them in a multi-stimulus bioreactor system which mimics the in-vivo shear flow environment and simultaneously supplies a magnetic field to improve cell proliferation. A cell culture result showed the superiority of combining our bioreactor system with the PPF/DEF 3D scaffold. The combination of 3D scaffold fabricated by Blu-ray based MSTL and a multi-stimulus bioreactor system may be a valuable tool for bone tissue regeneration.
Similar content being viewed by others
References
R. Lanza, R. Langer and J. P. Vacanti, Principles of tissue engineering, Academic Press: New York(1997).
R. Thomson, M. Yaszemski and A. G. Mikos, Polymer scaffold processing -Principles of tissue engineering, R.G. Landes Company: Texas(1997).
C. M. Agrwal, K. A. Athansiou and J. D. Heckman, Biodegradable PLA-PGA polymers for tissue engineering in orthopaedics, Mater. Sci. Forum, 250 (1997) 115–128.
R. Langer, Selected advances in drug delivery and tissue engineering, J. Control. Release, 62 (1999) 7–11.
L. Lu and A. G. Mikos, The importance of new processing techniques in tissue engineering, MRS Bull., 21 (1996) 28–32.
T. Ozdemir, A. M. Higgins and J. L. Brown, Osteoinductive biomaterial geometries for bone regenerative engineering, Curr. Pharm. Des., 19 (2013) 3446–3455.
W. Chen, Y. Tabata and Y. W. Tong, Fabricating tissue engineering scaffolds for simultaneous cell growth and drug delivery, Curr. Pharm. Des., 16 (2010) 2388–2394.
A. G. Mikos, A. J. Thorsen, L. A. Czerwonka, Y. Bao, R. Langer and D. N. Winslow, Preparation and characterization of poly (l-lactic acid) forms, Polymer, 35 (1994) 1068–1077.
A. G. Mikos, Y. Bao, L. G. Cima, D. E. Ingber, J. P. Vacanti and R. Langer, Preparation of poly (glycolic acid) bonded fiber structures for cell attachment and transplantation, J. Biomed. Mater. Res., 27 (1993) 183–189.
H. Hall, Modified fibrin hydrogel matrices: both, 3Dscaffolds and local and controlled release systems to stimulate angiogenesis, Curr. Pharm. Des., 13 (2007) 3597–3607.
G. M. Harris, K. Rutledge, Q. Cheng, J. Blanchette and E. Jabbarzadeh, Strategies to direct angiogenesis within scaffolds for bone tissue engineering, Curr. Pharm. Des., 19 (2013) 3456–3465.
E. Vanderleyden, S. Mullens, J. Luyten and P. Dubruel, Implantable (bio) polymer coated titanium scaffolds: a review, Curr. Pharm. Des., 18 (2012) 2576–2590.
A. G. Mikos, G. Sarakinos, S. M. Leite, J. P. Vacanti and R. Langer, Laminated three dimensional biodegradable foams for use in tissue engineering, Biomaterials, 14 (1993) 323–330.
D. J. Mooney, D. F. Baldwin, N. P. Suh, J. P. Vacanti and R. Langer, Novel approach to fabricate porous sponges of poly (D, L-lactic-co-glycolic acid) without the use of organic solvents, Biomaterials, 14 (1996) 1417–1422.
J. P. Kim, E. K. Park, S. Y. Kim, J. W. Shin and D. W. Cho, Fabrication of a SFF-based three-dimensional scaffold using a precision deposition system in tissue engineering, J. Micromech. Micoreng., 18 (2008) 055027.
J. W. Lee, P. X. Lan, B. Kim, G. Lim and D. W. Cho, Fabrication and characteristic analysis of a poly(propylene fumarate) scaffold using micro-stereolithography technology, J. Biomed. Mater. Res. B Appl. Biomater., 87 (2008) 1–9.
B. Leukers, H. Gülkan, S. H. Irsen, S. Milz, C. Tille, M. Schieker and H. Seitz, Hydroxyapatite scaffolds for bone tissue engineering made by 3D printing, J. Mater. Sci. Mater. Med., 16 (2005) 1121–1124.
K. Ikuta and S. Maruo, Submicron stereolithography for the production of freely movable mechanisms by using singlephoton polymerization, Sensor. Actuat. A-Phys., 100 (2002) 70–76.
F. P. W. Melchels, J. Feijen and D. W. Grijpma, A review on stereolithography and its applications in biomedical engineering, Biomaterials, 31 (2010) 6121–6130.
H. W. Kang, Y. S. Jeong, S. J. Lee, K. S. Kim and W. S. Yun, Development of a compact micro-stereolithography (MSTL) system using a Blu-ray optical pickup unit, J. Micromech. Microeng., 22 (2012) 115021.
D. D. Frazier, V. K. Lathi, T. N. Gerhart and W. C. Hayes, Ex vivo degradation of a poly (propylene glycol-fumarate) biodegradable particulate composite bone cement, J. Biomed. Mater. Res., 35 (1997) 383–389.
J. P. Fisher, D. Dean and A. G. Mikos, Photocrosslinking characteristics and mechanical properties of diethyl fumarate/ poly(propylene fumarate) biomaterials, Biomaterials, 23 (2002) 4333–4343.
J. W. Lee, J. W. Jung, D. S. Kim, G. B. Lim and D. W. Cho, Estimation of cell proliferation by various peptide coating at the PPF/DEF 3D scaffold, Microelectron. Eng., 86 (2009) 1451–1454.
X. Ba, M. Hadjiargyrou, E. DiMasi, Y. Meng, M. Simon, Z. Tan and M. H. Rafailovich, The role of moderate static magnetic fields on biomineralization of osteoblasts on sulfonated polystyrene films, Biomaterials, 32 (2011) 7831–7838.
S. M. Ross, Combined Dc and ELF magnetic fields can alter cell proliferation, Bioelectromagnetics, 11 (1990) 27–36.
S. H. Cartmell, B. D. Porter, A. J. García and R. E. Guldberg, Effects of medium perfusion rate on cell-seeded threedimensional bone constructs in vitro, Tissue Eng., 9 (2003) 1197–1203.
B. Carpentier, P. Layrolle and C. Legallais, Bioreactors for bone tissue engineering, Int. J. Artif. Organs, 34 (2011) 259–270.
S. Yang, K. F. Leong, Z. Du and C. K. Chua, The design of scaffolds for use in tissue engineering: part II. Rapid prototyping techniques, Tissue Eng., 7 (2002) 1–11.
Y. S. Jeong, Micro-structure fabrication using blu-ray based micro-stereolithography apparatus, Master Thesis, Graduate School of Knowledge-based Technology and Energy, Korea Polytechnic University(2011).
J. H. Kim, E. S. Choi, Y. S. Jeong and Y. S. Yun, Study on the micro stereolithography apparatus using Blu-ray OPU, KSMPE Conference Proceedings(2012).
C. E. Misch, Z. Qu and M. W. Bidez, Mechanical properties of trabecular bone in the human mandible: Implications for dental implant treatment planning and surgical placement, J. Oral Maxillofac. Surg., 57 (1999) 700–706.
L. D. Muiznieks and F. W. Keeley, Molecular assembly and mechanical properties of the extracellular matrix: A fibrous protein perspective, Biochim. Biophys. Acta, 1832 (2013) 866–875.
W. M. Elbjeirami, E. O. Yonter, B. C. Starcher and J. L. West, Enhancing mechanical properties of tissue-engineered constructs via lysyl oxidase crosslinking activity, J. Biomed. Mater. Res. A, 66 (2003) 513–521.
A. S. Scott, S. W. Patricia, G. Koyal, M. M. Jennifer, A. R. Isaac and L. B. Gary, The use of natural polymers in tissue engineering: a focus on electrospun extracellular matrix analogues, Polymers, 2 (2010) 522–553.
E. L. Hedberg, C. K. Shih, J. J. Lemoine, M. D. Timmer, M. A. Liebschner, J. A. Jansen and A. G. Mikos, In vitro degradation of porous poly(propylene fumarate)/poly(DL-lacticcoglycolic acid) composite scaffolds, Biomaterials, 26 (2005) 3215–3225.
Author information
Authors and Affiliations
Corresponding author
Additional information
Recommended by Associate Editor Won Hyoung Ryu
Jae-Hun Kim received his B.S. and M.S. degrees in Mechanical Engineering from the Korea Polytechnic University in 2012 and 2014. His research interests include 3D printing technology and its application to biomedical field such as medical device, tissue engineering and local drug delivery.
Jin Woo Lee received his Ph.D. degree in Mechanical Engineering from POSTECH in 2009. He is currently an Assistant Professor in the Department of Molecular Medicine at Gachon University. His research interests are realization of 3D micro-environment using 3D printing, and synthesis and modification of biomaterials.
Won-Soo Yun received his Ph.D. degree in Mechanical Engineering from POSTECH in 2000. He is currently a Professor in Department of Mechanical Engineering at Korea Polytechnic University. His research interests are on the advanced manufacturing system and tis biomedical applications.
Rights and permissions
About this article
Cite this article
Kim, JH., Lee, J.W. & Yun, WS. Fabrication and tissue engineering application of a 3D PPF/DEF scaffold using Blu-ray based 3D printing system. J Mech Sci Technol 31, 2581–2587 (2017). https://doi.org/10.1007/s12206-017-0456-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12206-017-0456-y