Abstract
Temperature of microfluidic system is greatly sensitive because of fast heat conduction and small heat capacity due to the scale effect. The purpose of this study is the development of a measurement system for the temperature field of liquids in a microfluidic device with high spatial- and temporal-resolution. Measurement method employed in this study is laser-induced fluorescence using fluorescein with the temperature dependence of fluorescent intensity. In order to measure the transient temperature field, an image-intensified high-speed camera was utilized. The signal-to-noise ratio can be improved by the time- or phase-averaging scheme. Applying the synchronization mechanism, phase-averaged temperature data with the time resolution of 500 μs can be obtained. Spatial resolution estimated from the Rayleigh limit was approximately 530 nm. The validity of the developed measurement system was confirmed by the experiments for the transient behavior of the liquid temperature undergoing the laser heating in the microfluidic device.
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Masahiro Motosuke received his B. S. degree from Department of System Design Engineering at Keio University, Japan, in 2001. He received his M. S. and Ph. D degrees from Keio University in 2003 and 2006. He is now an Assistant Professor at Department of Mechanical Engineering in Tokyo University of Science, Japan. His research interests are in the development of advanced sensing and control for the fluid or particle motion and properties in a microfluidic system based on optical or electrokinetic approach.
Dai Akutsu received his B. S. degree in Mechanical Engineering from Tokyo University of Science, Japan, in 2008. He is currently in the master course of the graduate school of Mechanical Engineering in Tokyo University of Science. His research interests include the temperature measurement of the highly tiny region in a microfluidic device using micro-LIF and the development of micro- mixer/ sorter by means of electrokinetics.
Shinji Honami received his B. S. in Mechanical Engineering from Keio University, Japan, in 1967. He received his M. S. and Ph. D degrees from Keio University in 1969 and 1974, respectively. He is a Professor at School of Engineering, Department of Mechanical Engineering, Tokyo University of Science, Japan. His research interests include turbulent and laminar flow control and microfluidics.
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Motosuke, M., Akutsu, D. & Honami, S. Temperature measurement of microfluids with high temporal resolution by laser-induced fluorescence. J Mech Sci Technol 23, 1821–1828 (2009). https://doi.org/10.1007/s12206-009-0609-8
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DOI: https://doi.org/10.1007/s12206-009-0609-8