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Cloning and expression of a transcription factor activator protein-1 member identified from the swimming crab Portunus trituberculatus

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Cell Stress and Chaperones Aims and scope

Abstract

Transcription activator proteins are regulatory proteins that bind to the promoter regions of target genes. Transcription activator protein-1 (AP-1) regulates numerous genes related to the immune system, apoptosis, and proliferation. In this study, the full-length cDNA of AP-1 from Portunus trituberculatus (PtAP-1) was identified by expressed sequence tag analysis and cDNA-end rapid amplification. The gene is 1183 bp and encodes a 256-amino acid protein with a predicted molecular mass and isoelectric point of 28.96 kDa and 8.90, respectively. PtAP-1 showed the highest expression level in the gonad tissue and the lowest expression level in blood, hemocyte, muscle, hepatopancreas, and gill, during the first 6 h of low-salinity stimulation (10%). Additionally, we observed steady decreases in PtAP-1 mRNA expression in the gill, but at 12 h, expression was initially upregulated, followed by a significant decrease until restoration to baseline levels at 48 h. Additionally, Vibrio alginolyticus challenge resulted in significant upregulation of PtAP-1 expression in the first 6 h, which was maintained at high levels for 48 h. From 48 to 72 h, we observed decreases in PtAP-1 levels, although they remained significantly higher than those detected at baseline. These results suggested that PtAP-1 is involved in the immune response and osmoregulation of crustaceans.

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Acknowledgments

We are grateful to the anonymous reviewers for their professional revision of the manuscript.

Funding

This study was sponsored by the National Natural Science Foundation of China (41476124), China Postdoctoral Science Foundation (2018M632439), Ningbo university research fund (XYL18013), and K.C. Wong Magna Fund in Ningbo University.

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Authors and Affiliations

  1. Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, 315211, People’s Republic of China

    Huan Wang, Ce Shi, Mengyao Kong, Changkao Mu, Hongling Wei & Chunlin Wang

  2. Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo, 315211, People’s Republic of China

    Huan Wang, Ce Shi, Changkao Mu & Chunlin Wang

Authors
  1. Huan Wang
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  2. Ce Shi
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  3. Mengyao Kong
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  4. Changkao Mu
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  5. Hongling Wei
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  6. Chunlin Wang
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Contributions

C Wang and CK Mu conceived and designed the study. H Wang and MY Kong performed the cultivation of experimental animals. H Wang, C Shi, MY Kong, C Mu, and HL Wei performed and analyzed all the other experiments. H Wang and C Shi wrote the manuscript with support from all authors. All authors read and approved the final manuscript.

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Correspondence to Ce Shi or Chunlin Wang.

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The authors declare that they have no competing interests.

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The animal subjects used in the present study are crabs, which are invertebrates and are exempt from this requirement.

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Wang, H., Shi, C., Kong, M. et al. Cloning and expression of a transcription factor activator protein-1 member identified from the swimming crab Portunus trituberculatus. Cell Stress and Chaperones 23, 1275–1282 (2018). https://doi.org/10.1007/s12192-018-0935-9

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  • DOI: https://doi.org/10.1007/s12192-018-0935-9

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