Abstract
Mammalian hibernators survive low body temperatures, ischemia–reperfusion, and restricted nutritional resources via global reductions in energy-expensive cellular processes and selective increases in stress pathways. Consequently, studies that analyze hibernation uncover mechanisms which balance metabolism and support survival by enhancing stress tolerance. We hypothesized processing factors that influence messenger ribonucleic acid (mRNA) maturation and translation may play significant roles in hibernation. We characterized the amino acid sequences of three RNA processing proteins (T cell intracellular antigen 1 (TIA-1), TIA1-related (TIAR), and poly(A)-binding proteins (PABP-1)) from thirteen-lined ground squirrels (Ictidomys tridecemlineatus), which all displayed a high degree of sequence identity with other mammals. Alternate Tia-1 and TiaR gene variants were found in the liver with higher expression of isoform b versus a in both cases. The localization of RNA-binding proteins to subnuclear structures was assessed by immunohistochemistry and confirmed by subcellular fractionation; TIA-1 was identified as a major component of subnuclear structures with up to a sevenfold increase in relative protein levels in the nucleus during hibernation. By contrast, there was no significant difference in the relative protein levels of TIARa/TIARb in the nucleus, and a decrease was observed for TIAR isoforms in cytoplasmic fractions of torpid animals. Finally, we used solubility tests to analyze the formation of reversible aggregates that are associated with TIA-1/R function during stress; a shift towards the soluble fraction (TIA-1a, TIA-1b) was observed during hibernation suggesting enhanced protein aggregation was not present during torpor. The present study identifies novel posttranscriptional regulatory mechanisms that may play a role in reducing translational rates and/or mRNA processing under unfavorable environmental conditions.
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References
Afonina E, Stauber R, Pavlakis GN (1998) The human poly(A)-binding protein 1 shuttles between the nucleus and the cytoplasm. J Biol Chem 273:13015–13021. doi:10.1074/jbc.273.21.13015
Anderson P, Kedersha N (2002) Visibly stressed: the role of eIF2, TIA-1, and stress granules in protein translation. Cell Stress Chaperones 7:213–221. doi:10.1379/1466-1268(2002)
Biggar KK, Storey KB (2011) The emerging roles of microRNAs in the molecular responses of metabolic rate depression. J Mol Cell Biol 3:167–175. doi:10.1093/jmcb/mjq045
Bouma HR, Verhaag EM, Otis JP, Heldmaier G, Swoap SJ, Strijkstra AM, Henning RH, Carey HV (2012) Induction of torpor: mimicking natural metabolic suppression for biomedical applications. J Cell Physiol 227:1285–1290. doi:10.1002/jcp.22850
Burd CG, Matunis EL, Dreyfuss G (1991) The multiple RNA-binding domains of the mRNA poly(A)-binding protein have different RNA-binding activities. Mol Cell Biol 11:3419–3424
Caponigro G, Parker R (1995) Multiple functions for the poly(A)-binding protein in mRNA decapping and deadenylation in yeast. Genes Dev 9:2421–2432. doi:10.1101/gad.9.19.2421
Chung D, Lloyd GP, Thomas RH, Guglielmo CG, Staples JF (2011) Mitochondrial respiration and succinate dehydrogenase are suppressed early during entrance into a hibernation bout, but membrane remodeling is only transient. J Comp Physiol B 181:699–711. doi:10.1007/s00360-010-0547-x
Dave KR, Christian SL, Perez-Pinzon MA, Drew KL (2012) Neuroprotection: lessons from hibernators. Comp Biochem Physiol B Biochem Mol Biol 162:1–9. doi:10.1016/j.cbpb.2012.01.008
Decker CJ, Parker R (1993) A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation. Genes Dev 7:1632–1643. doi:10.1101/gad.7.8.1632
Förch P, Puig O, Kedersha N, Martínez C, Granneman S, Séraphin B, Anderson P, Valcárcel J (2001) The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing. Mol Cell 6:1089–1098. doi:10.1016/S1097-2765(00)00107-6
Frerichs KU, Smith CB, Brenner M, DeGracia DJ, Krause GS, Marrone L, Dever TE, Hallenbeck JM (1998) Suppression of protein synthesis in brain during hibernation involves inhibition of protein initiation and elongation. Proc Natl Acad Sci U S A 95:14511–14516. doi:10.1073/pnas.95.24.14511
Geiser F (2004) Metabolic rate and body temperature reduction during hibernation and daily torpor. Annu Rev Physiol 66:239–274. doi:10.1146/annurev.physiol.66.032102.115105
Gilks N, Kedersha N, Ayodele M, Shen L, Stoecklin G, Dember LM, Anderson P (2004) Stress granule assembly is mediated by prion-like aggregation of TIA-1. Mol Biol Cell 15:5383–5398. doi:10.1091/mbc.E04-08-0715
Heldmaier G, Ortmann S, Elvert R (2004) Natural hypometabolism during hibernation and daily torpor in mammals. Respir Physiol Neurobiol 141:317–329. doi:10.1016/j.resp.2004.03.014
Hittel D, Storey KB (2002) The translation state of differentially expressed mRNAs in the hibernating thirteen-lined ground squirrel (Spermophilus tridecemlineatus). Arch Biochem Biophys 401:244–254. doi:10.1016/S0003-9861(02)00048-6
Izquierdo JM, Valcárcel J (2007) Two isoforms of the T-cell intracellular antigen 1 (TIA-1) splicing factor display distinct splicing regulation activities. Control of TIA-1 isoform ratio by TIA-1-related protein. J Biol Chem 282:19410–19417. doi:10.1074/jbc.M700688200
Kawakami A, Tian Q, Streuli M, Poe M, Edelhoff S, Disteche CM, Anderson P (1994) Intron-exon organization and chromosomal localization of the human TIA-1 gene. J Immunol 152:4937–4945
Kedersha N, Anderson P (2007) Mammalian stress granules and processing bodies. Methods Enzymol 431:61–81. doi:10.1016/S0076-6879(07)31005-7
Kornfeld SF, Biggar KK, Storey KB (2012) Differential expression of mature microRNAs involved in muscle maintenance of hibernating little brown bats, Myotis lucifugus: a model of muscle atrophy resistance. Genomics Proteomics Bioinforma 10:295–301. doi:10.1016/j.gpb.2012.09.001
López de Silanes I, Galbán S, Martindale JL, Yang X, Mazan-Mamczarz K, Indig FE, Falco G, Zhan M, Gorospe M (2005) Identification and functional outcome of mRNAs associated with RNA-binding protein TIA-1. Mol Cell Biol 25:9520–9531. doi:10.1128/MCB.25.21.9520-9531.2005
Malatesta M, Zancanaro C, Martin TE, Chan EKL, Almaric F, Luhrmann R, Vogel P, Fakan S (1994) Cytochemical and immunocytochemical characterization of nuclear bodies during hibernation. Eur J Cell Biol 65:82–93
Malatesta M, Cardinali A, Battistelli S, Zancanaro C, Martin TE, Fakan S, Gazzanelli G (1999) Nuclear bodies are usual constituents in tissues of hibernating dormice. Anat Rec 254:389–395. doi:10.1002/(SICI)1097-0185(19990301)254:3<389::AID-AR10>3.0.CO;2-E
Malatesta M, Luchetti F, Marcheggiani F, Fakan S, Gazzanelli G (2001) Disassembly of nuclear bodies during arousal from hibernation: an in vitro study. Chromosoma 110:471–477. doi:10.1007/s004120100166
Malatesta M, Biggiogera M, Baldelli B, Barabino SM, Martin TE, Zancanaro C (2008) Hibernation as a far-reaching program for the modulation of RNA transcription. Microsc Res Tech 71:564–572. doi:10.1002/jemt.20587
Martin SL, Maniero GD, Carey C, Hand SC (1999) Reversible depression of oxygen consumption in isolated liver mitochondria during hibernation. Physiol Biochem Zool 72:255–264. doi:10.1086/316667
Mazan-Mamczarz K, Ashish L, Martindale JL, Kawai T, Gorospe M (2006) Translational repression by RNA-binding protein TIAR. Mol Cell Biol 26:2716–2727. doi:10.1128/MCB.26.7.2716-2727.2006
McArthur MD, Milsom WK (1991) Changes in ventilation and respiratory sensitivity associated with hibernation in Columbian (Spermophilus columbianus) and golden-mantled (Spermophilis lateralis) ground squirrels. Physiol Zool 64:940–959
McMullen DC, Hallenbeck JM (2010) Regulation of Akt during torpor in the hibernating ground squirrel, Ictidomys tridecemlineatus. J Comp Physiol B 180:927–934. doi:10.1007/s00360-010-0468-8
Minvielle-Sebastia L, Preker PJ, Wiederkehr T, Strahm Y, Keller W (1997) The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3′-end formation. Proc Natl Acad Sci U S A 94:7897–7902. doi:10.1073/pnas.94.15.7897
Moore MJ (2005) From birth to death: the complex lives of eukaryotic mRNAs. Science 309:1514–1518. doi:10.1126/science.1111443
Morin P Jr, Storey KB (2006) Evidence for a reduced transcriptional state during hibernation in ground squirrels. Cryobiology 53:310–318. doi:10.1016/j.cryobiol.2006.08.002
Morin P Jr, Storey KB (2009) Mammalian hibernation: differential gene expression and novel application of epigenetic controls. Int J Dev Biol 53:433–442. doi:10.1387/ijdb.082643pm
Pan P, van Breukelen F (2011) Preference of IRES-mediated initiation of translation during hibernation in golden-mantled ground squirrels, Spermophilus lateralis. Am J Physiol Regul Integr Comp Physiol 301:R370–R377. doi:10.1152/ajpregu.00748.2010
Piecyk M, Wax S, Beck ARP, Kedersha N, Gupta M, Maritim B, Chen S, Gueydan C, Kruys V, Streuli M, Anderson P (2000) TIA-1 is a translational silencer that selectively regulates the expression of TNF-α. EMBO J 19:4154–4163. doi:10.1093/emboj/19.15.4154
Ripaud L, Maillet L, Cullin C (2003) The mechanisms of [URE3] prion elimination demonstrate that large aggregates of Ure2p are dead-end products. EMBO J 22:5251–5259. doi:10.1093/emboj/cdg488
Rouble AN, Helfer J, Mamady H, Storey KB, Tessier SN (2013) Anti-apoptotic signaling as a cytoprotective mechanism in mammalian hibernation. Peer J 1:e29. doi:10.7717/peerj.29
Sachs AB, Davis RW (1989) The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation. Cell 58:857–867. doi:10.1016/0092-8674(89)90938-0
Staples JF (2011) Maintaining metabolic balance in mammalian hibernation and daily torpor. In: Nowakowska A, Caputa M (eds) Hypometabolism: strategies of survival in vertebrates and invertebrates. Research Signpost, Kerala, pp 95–115
Storey KB (1996) Oxidative stress: animal adaptations in nature. Braz J Med Biol Res 29:1715–1733
Storey KB (2003) Mammalian hibernation: transcriptional and translational control. In: Roach RC, Wagner PD, Hackett PH (eds) Hypoxia: through the lifecycle. Kluwer Academic/Plenum Publishers, New York, pp 7–24
Storey KB (2010) Out cold: biochemical regulation of mammalian hibernation—a mini-review. Gerontology 56:220–230. doi:10.1159/000228829
Storey KB, Storey JM (2004) Metabolic rate depression in animals: transcriptional and translational controls. Biol Rev Camb Philos Soc 79:207–233. doi:10.1017/S1464793103006195
Storey KB, Storey JM (2010) Metabolic rate depression: the biochemistry of mammalian hibernation. In: Makowski GS (ed) Advances in clinical chemistry. Academic, Burlington, pp 77–108
Suswam EA, Li YY, Mahtani H, King PH (2005) Novel DNA-binding properties of the RNA-binding protein TIAR. Nucleic Acids Res 33:4507–4518. doi:10.1093/nar/gki763
Taupin JL, Tian Q, Kedersha N, Robertson M, Anderson P (1995) The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death. Proc Natl Acad Sci U S A 92:1629–1633. doi:10.1073/pnas.92.5.1629
Tessier SN, Storey KB (2010) Expression of myocyte enhancer factor-2 and downstream genes in ground squirrel skeletal muscle during hibernation. Mol Cell Biochem 344:151–162. doi:10.1007/s11010-010-0538-y
Wang LCH, Lee TF (1996) Torpor and hibernation in mammals: metabolic, physiological, and biochemical adaptations. In: Fregley MJ, Blatteis CM (eds) Handbook of physiology: environmental physiology. Oxford University Press, New York, pp 507–532
Wolozin B (2012) Regulated protein aggregation: stress granules and neurodegeneration. Mol Neurodegener 7:56. doi:10.1186/1750-1326-7-56
Wu CW, Storey KB (2012) Regulation of the mTOR signaling network in hibernating thirteen-lined ground squirrels. J Exp Biol 215:1720–1727. doi:10.1242/jeb.066225
Zatzman ML (1984) Renal and cardiovascular effects of hibernation and hypothermia. Cryobiology 21:593–614. doi:10.1016/0011-2240(84)90220-7
Zhang T, Delestienne N, Huez G, Kruys V, Gueydan C (2005) Identification of the sequence determinants mediating the nucleo-cytoplasmic shuttling of TIAR and TIA-1 RNA-binding proteins. J Cell Sci 118:5453–5463. doi:10.1242/jcs.02669
Acknowledgments
We would like to thank Dr. J.M. Hallenbeck and Dr. D.C. McMullen (NINDS, NIH, Bethesda) for providing the tissue samples for this study. Thanks also to J.M. Storey for editorial review of the manuscript. Research was supported by a grant from the Canadian Institute of Health Research (CIHR) to SL, a discovery grant from the Natural Sciences and Engineering Research Council (NSERC) of Canada to KBS, and the Canada Research Chairs program. SNT held an NSERC PGSD scholarship.
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Tessier, S.N., Audas, T.E., Wu, CW. et al. The involvement of mRNA processing factors TIA-1, TIAR, and PABP-1 during mammalian hibernation. Cell Stress and Chaperones 19, 813–825 (2014). https://doi.org/10.1007/s12192-014-0505-8
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DOI: https://doi.org/10.1007/s12192-014-0505-8