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Molecular and functional characterization of the only known hemiascomycete ortholog of the carboxyl terminus of Hsc70-interacting protein CHIP in the yeast Yarrowia lipolytica

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Cell Stress and Chaperones Aims and scope

Abstract

The carboxyl terminus of Hsc70-interacting protein (CHIP) is an Hsp70 co-chaperone and a U-box ubiquitin ligase that plays a crucial role in protein quality control in higher eukaryotes. The yeast Yarrowia lipolytica is the only known hemiascomycete where a CHIP ortholog is found. Here, we characterize Y. lipolytica’s CHIP ortholog (Yl.Chn1p) and document its interactions with components of the protein quality control machinery. We show that Yl.Chn1p is non-essential unless Y. lipolytica is severely stressed. We sought for genetic interactions among key components of the Y. lipolytica protein quality control arsenal, including members of the Ssa-family of Hsp70 molecular chaperones, the Yl.Bag1p Hsp70 nucleotide exchange factor, the Yl.Chn1p and Yl.Ufd2p U-box ubiquitin ligases, the Yl.Doa10p and Yl.Hrd1p RING-finger ubiquitin ligases, and the Yl.Hsp104p disaggregating molecular chaperone. Remarkably, no synthetic phenotypes were observed among null alleles of the corresponding genes in most cases, suggesting that overlapping pathways efficiently act to enable Y. lipolytica cells to survive under harsh conditions. Yl.Chn1p interacts with mammalian and Saccharomyces cerevisiae members of the Hsp70 family in vitro, and these interactions are differently regulated by Hsp70 co-chaperones. We demonstrate notably that Yl.Chn1p/Ssa1p interaction is Fes1p-dependent and the formation of an Yl.Chn1p/Ssa1p/Sse1p ternary complex. Finally, we show that, similar to Sse1p, Yl.Chn1p can act as a “holdase” to prevent the aggregation of a heat-denatured protein.

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Acknowledgments

We thank Andreas Bracher (Max Plank Institut für Biochemie, Martinsried, Germany) for providing Fes1p, Fes1-A79R/R195A, and Sse1p expression plasmids. We thank Kenza Lahkim-Bennani for her contribution at the initial stages of this work. This work was supported by the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Recherche Agronomique (INRA), AgroParisTech and by a research grant FC0518 from the French cystic fibrosis foundation Vaincre la Mucoviscidose (to MK). CNM was the beneficiary of a doctoral grant from the French Ministry of Research.

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  1. Céline N. Martineau

    Present address: Department of Genetics, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9700 RB, Groningen, The Netherlands

Authors and Affiliations

  1. Laboratoire d’Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Bâtiment 34, Avenue de la Terrasse, 91190, Gif-sur-Yvette, France

    Céline N. Martineau, Ronald Melki & Mehdi Kabani

  2. Laboratoire de Microbiologie de l’Alimentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique, UMR1319, 78350, Jouy-en-Josas, France

    Marie-Thérèse Le Dall & Jean-Marie Beckerich

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  1. Céline N. Martineau
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Correspondence to Mehdi Kabani.

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Céline N. Martineau and Marie-Thérèse Le Dall these authors contributed equally to this work.

Part of this work was carried out at the former Laboratoire de Microbiologie et Génétique Moléculaire, CNRS, AgroParisTech, INRA UMR2585, F-78850, Thiverval-Grignon, France.

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Martineau, C.N., Le Dall, MT., Melki, R. et al. Molecular and functional characterization of the only known hemiascomycete ortholog of the carboxyl terminus of Hsc70-interacting protein CHIP in the yeast Yarrowia lipolytica . Cell Stress and Chaperones 17, 229–241 (2012). https://doi.org/10.1007/s12192-011-0302-6

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