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Effects of chronic heat stress on the expressions of heat shock proteins 60, 70, 90, A2, and HSC70 in the rabbit testis

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Cell Stress and Chaperones Aims and scope

Abstract

Few studies have focused on the expression of heat shock proteins (HSPs) after chronic heat stress. The objective of this study was to investigate the effect of chronic high temperature–humidity index treatment on the expressions of HSP60, HSP70, HSP90, HSPA2 and HSC70, in the Rex rabbit testis and the expressions of these proteins after recovery from the chronic heat shock. Thirty mature male rabbits of the same age were randomly divided into three groups: control, heat stress, and recovery. The western blot results showed that the expressional levels of HSP60, HSP90, and HSC70 increased significantly and HSPA2 was elevated slightly after a 9-week heat treatment. HSP70 was absent in the control testis and had a high level of expression after heat stress. All of these proteins partially reverted back to normal levels after a 9-week recovery. The immunohistochemical results indicated that the expression patterns of HSP60, HSP90, HSPA2, and HSC70 did not change.

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Abbreviations

HSP:

Heat shock protein

THI:

Temperature–humidity index

HSC:

Heat shock cognate protein

CON:

Control

HS:

Heat stress

RE:

Heat stress recovery

IHC:

Immunohistochemistry

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Acknowledgments

This work was supported by the Earmarked Fund for Modern Agro-Industry Technology Research System (CARS-44) and the Natural Science Foundation of China (31001009).

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Authors and Affiliations

  1. College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People’s Republic of China

    Yangli Pei, Yingjie Wu & Yinghe Qin

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  1. Yangli Pei
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  2. Yingjie Wu
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  3. Yinghe Qin
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Correspondence to Yinghe Qin.

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Yangli Pei and Yingjie Wu contributed equally to this work.

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Pei, Y., Wu, Y. & Qin, Y. Effects of chronic heat stress on the expressions of heat shock proteins 60, 70, 90, A2, and HSC70 in the rabbit testis. Cell Stress and Chaperones 17, 81–87 (2012). https://doi.org/10.1007/s12192-011-0287-1

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  • DOI: https://doi.org/10.1007/s12192-011-0287-1

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