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Proteome profiling of heat shock of human primary breast epithelial cells, a dataset report

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Cell Stress and Chaperones Aims and scope

Abstract

Exposure to elevated temperatures has a strong effect on cell functions, and is used in clinical practice. Hyperthermia may affect multiple regulatory mechanisms in cells. To understand better the response to hyperthermia of immortalized primary human breast epithelial cells, we performed a proteomics study of these cells cultured at 34°C or 39°C. Twenty-four proteins were shown to be differentially expressed due to hyperthermia. Analysis of these proteins showed the potential involvement of various biological processes in response to hyperthermia, e.g., cell adhesion, cell communication, and cell cycle. Transforming growth factor-β2 (TGF-β2) and heat shock protein 27 (HSP27) were found to be upregulated at 39°C. TGF-β2 was found to affect expression of HSP27, and to have a protective role in hyperthermia-induced cell death. Thus, the dataset described here of hyperthermia-related proteins in human primary breast epithelial cells predicts a number of cellular activities affected by exposure to high temperatures and provides a set of proteins for further studies.

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References

  • Beere HM (2004) “The stress of dying”: the role of heat shock proteins in the regulation of apoptosis. J Cell Sci 117(Pt 13):2641–2651

    Article  PubMed  CAS  Google Scholar 

  • Burke JJ, Orzech KA (1988) The heat-shock response in higher plants: a biochemical model. Plant Cell Environ 11:441–444

    Article  CAS  Google Scholar 

  • Chen F, Resavi R, Wang CC et al (2007) Proteasome inhibition potentiates the cytotoxic effects of hyperthermia in HT-29 colon cancer cells through inhibition of heat shock protein 27. Oncology 73:98–103

    Article  PubMed  CAS  Google Scholar 

  • Di K, Wong YC, Wang X (2007) Id-1 promotes TGF-beta1-induced cell motility through HSP27 activation and disassembly of adherens junction in prostate epithelial cells. Exp Cell Res 313(19):3983–3999

    Article  PubMed  CAS  Google Scholar 

  • Flanders KC, Winokur TS, Holder MG et al (1993) Hyperthermia induces expression of transforming growth factor-betas in rat cardiac cells in vitro and in vivo. J Clin Invest 92:404–410

    Article  PubMed  CAS  Google Scholar 

  • Jia M, Souchelnytskyi N, Hellman U et al (2010) Proteome profiling of immortalization-to-senescence transition of human breast epithelial cells identified MAP2K3 as a senescence-promoting protein which is downregulated in human breast cancer. Proteomics Clin Appl 4:816–828. doi:10.1002/prca.201000006

    Article  PubMed  CAS  Google Scholar 

  • Karmazyn M, Mailer K, Currie RW (1990) Acquisition and decay of heat-shocked-enhanced postischemic ventricular recovery. Am J Physiol 259:424–431

    Google Scholar 

  • Kwon SM, Kim SA, Yoon JH et al (2010) Transforming growth factor beta1-induced heat shock protein 27 activation promotes migration of mouse dental papilla-derived MDPC-23 cells. J Endod 36(8):1332–1335

    Article  PubMed  Google Scholar 

  • Lanneau D, Brunet M, Frisan E et al (2008) Heat shock proteins: essential proteins for apoptosis regulation. J Cell Mol Med 12(3):743–761

    Article  PubMed  CAS  Google Scholar 

  • Linquist S, Craig EA (1988) The heat shock proteins. Annu Rev Genet 22:631–677

    Article  Google Scholar 

  • Lüders S, Fallet C, Franco-Lara E (2009) Proteome analysis of the Escherichia coli heat shock response under steady-state conditions. Proteome Sci 7:36

    Article  PubMed  Google Scholar 

  • Mathew A, Morimoto RI (1998) Role of the heat-shock response in the life and death of proteins. Ann NY Acad Sci 851:99–111

    Article  PubMed  CAS  Google Scholar 

  • Movahedi S, Waites W (2000) A two-dimensional protein gel electrophoresis study of the heat stress response of Bacillus subtilis Cells during sporulation. J Bacteriol 182:4758–4763

    Article  PubMed  CAS  Google Scholar 

  • O'Hare MJ, Bond J, Clarke C et al (2001) Conditional immortalization of freshly isolated human mammary fibroblasts and endothelial cells Comparison of early passage, senescent and hTERT immortalized endothelial cells. Proc Natl Acad Sci USA 98:646–651

    Article  PubMed  Google Scholar 

  • Pirkkala L, Nykänen P, Sistonen L (2001) Roles of the heat shock transcription factors in regulation of the heat shock response and beyond. FASEB J 15:1118–1131

    Article  PubMed  CAS  Google Scholar 

  • Schultz-Cherry S, Chen H, Mosher DF et al (1995) Regulation of transforming growth factor-beta activation by discrete sequences of thrombospondin 1. J Biol Chem 270(13):7304–7310

    Article  PubMed  CAS  Google Scholar 

  • Souchelnitskiy S, Chambaz EM, Feige JJ (1995) Thrombospondins selectively activate one of the two latent forms of transforming growth factor-beta present in adrenocortical cell-conditioned medium. Endocrinology 136(11):5118–5126

    Article  PubMed  CAS  Google Scholar 

  • Sreedhar AS, Csermely P (2004) Heat shock proteins in the regulation of apoptosis: new strategies in tumor therapy: a comprehensive review. Pharmacol Ther 101(3):227–257

    Article  PubMed  CAS  Google Scholar 

  • Stasyk T, Dubrovska A, Lomnytska M et al (2005) Phosphoproteome profiling of (TGF)-beta signaling: abrogation of TGFbeta1-dependent phosphorylation of transcription factor-II-I (TFII-I) enhances cooperation of TFII-I and Smad3 in transcription. Mol Biol Cell 16:4765–4780

    Article  PubMed  CAS  Google Scholar 

  • Stewart GR, Wernisch L, Stabler R et al (2002) Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays. Microbiology 148:3129–3138

    PubMed  CAS  Google Scholar 

  • Tomanek L (2002) The heat-shock response: its variation, regulation and ecological importance in intertidal gastropods (genus Tegula). Integ Comp Biol 42:797–807

    Article  CAS  Google Scholar 

  • Tomanek L (2005) Two-dimensional gel analysis of the heat-shock response in marine snails (genus Tegula): interspecific variation in protein expression and acclimation ability. J Exp Biol 208:3133–3143

    Article  PubMed  CAS  Google Scholar 

  • Yang YL, Chuang LY, Guh JY et al (2004) Thrombospondin-1 mediates distal tubule hypertrophy induced by glycated albumin. Biochem J 379:89–97

    Article  PubMed  CAS  Google Scholar 

  • Yu AL, Fuchshofer R, Birke M et al (2008) Oxidative stress and TGF-beta2 increase heat shock protein 27 expression in human optic nerve head astrocytes. Invest Ophthalmol Vis Sci 49(12):5403–5411

    Article  PubMed  Google Scholar 

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Acknowledgments

We wish to thank Parmjit Jat for the kind provision of cultures of immortalized human breast epithelial cells. We are also grateful to Ulf Hellman for the access to and advice with mass spectrometry. This project is partially supported by grants from the Karolinska Biomics Center, Department of Oncology-Pathology, Karolinska Institutet, Ludwig Institute for Cancer Research, the Swedish Research Council, the Swedish Institute, and the Swedish Cancer Society.

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Authors and Affiliations

  1. Department of Oncology-Pathology, Karolinska Biomics Center, Karolinska Institutet, SE-17176, Stockholm, Sweden

    Min Jia & Serhiy Souchelnytskyi

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  1. Min Jia
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  2. Serhiy Souchelnytskyi
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Correspondence to Min Jia.

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Supplemental Fig. 1

Graphical representation of betweenness and degree calculated for nodes in the whole network formed by the identified proteins. The plots were built with centiscape. a The plot shows distribution of nodes in relation to their values of betweenness. b The plot shows distribution of nodes in relation to their values of degree. (PDF 188 kb)

Supplemental Fig. 2

Network modules extracted from the whole network. Nine modules are shown a–i Network modules were generated by analysis of the whole network (Fig. 3). The network modules were extracted using an MCODE. (PDF 458 kb)

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Jia, M., Souchelnytskyi, S. Proteome profiling of heat shock of human primary breast epithelial cells, a dataset report. Cell Stress and Chaperones 16, 459–467 (2011). https://doi.org/10.1007/s12192-010-0253-3

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  • DOI: https://doi.org/10.1007/s12192-010-0253-3

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